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Adhesion, proliferation and differentiation of human mesenchymal stem cell on chitosan/collagen composite scaffold

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
  • Published:
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Abstract

In vitro tissue engineering requires a progenitor cell source and a porous scaffold providing three dimensional (3D) supports for growth and differentiation to attain tissue architectures. This research focused on fabrication and characterization of 3D porous scaffolds using chitosan (CS), collagen (CG) and chitosan-collagen (CS-CG) composite to investigate their influence on human mesenchymal stem cell (hMSC) adhesion, proliferation and differentiation. Material dependent variations in porous morphology and mechanical behavior of the fabricated CS, CG and CS-CG scaffold showed significant impact on hMSC adhesion, proliferation and differentiation. The maximum hMSC adhesion and proliferation was reported on CS-CG scaffold among all fabricated scaffold groups. Interconnectivity of pores structure in CS-CG scaffold was considered as preferable attribute for such enhanced growth and distribution throughout the scaffold. Besides, CS scaffold with well interconnected pores showed poor adhesion and proliferation because of inadequate adhesion motifs. In case of CG scaffold, optimum growth and distribution of hMSC occurs only at the surface because of the absence of interconnectivity in their pore structures. Likewise, osteogenic differentiation of hMSC occurs most preferably in CS-CG composite scaffold among all scaffold groups. Such enhanced hMSC proliferation and differentiation in CS-CG scaffold significantly influenced on mechanical behavior of scaffold which is essential for in vivo application of a bone tissue implant. Thus CS-CG composite scaffold holds promise to be a suitable platform for in vitro engineering of bone tissue implant.

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Acknowledgements

This work was supported by the Japanese Society for the Promotion of Science (JSPS) postdoctoral research fellowship for foreign researcher grant (P 12380), and JSPS KAKENHI Grant Number 24•02380.

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Authors and Affiliations

  1. Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensigh, 2202, Bangladesh

    Md. Abdul Kafi & Mst. Khudishta Aktar

  2. Research Institute for Applied Mechanics, Kyushu University, Kasuga, Fukuoka, Japan

    Md. Abdul Kafi & Mitsugu Todo

  3. Department of Dermatology, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan

    Mst. Khudishta Aktar

  4. Interdisciplinary Graduate School of Engineering Sciences, Kyushu University, Kasuga, Fukuoka, Japan

    Yos Phanny & Mitsugu Todo

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  1. Md. Abdul Kafi
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  2. Mst. Khudishta Aktar
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  4. Mitsugu Todo
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Correspondence to Md. Abdul Kafi.

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Kafi, M.A., Aktar, M.K., Phanny, Y. et al. Adhesion, proliferation and differentiation of human mesenchymal stem cell on chitosan/collagen composite scaffold. J Mater Sci: Mater Med 30, 131 (2019). https://doi.org/10.1007/s10856-019-6341-8

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  • DOI: https://doi.org/10.1007/s10856-019-6341-8

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