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Angiopoietin-1 Protects Spinal Cord Ischemia and Reperfusion Injury by Inhibiting Autophagy in Rats

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Abstract

Spinal cord ischemia and reperfusion (SCIR) injury can induce autophagy, which is involved in the survival of neurons. However, whether autophagy plays a neuroprotective or a detrimental role in SCIR injury remains controversial. Angiopoietin-1 (Ang-1), an endothelial growth factor, has been shown to have neuroprotective effects. The present study aimed to explore the neuroprotective mechanisms of Ang-1 in neuronal cells in a rat model of SCIR injury in vivo. Ang-1 protein and rapamycin were injected intrathecally. Basso Beattie Bresnahan (BBB) scoring and hematoxylin and eosin staining were used to assess the degree of SCIR injury. Proteins that reflected the level of autophagy expression, such as Beclin-1 and LC3, were evaluated by western blotting. The results indicated that SCIR injury resulted in loss in lower limb motor function. Ang-1 protein inhibited the expression of Beclin-1 and LC3, which improved the BBB score and alleviated spinal cord injury. In contrast, rapamycin, an autophagy activator, caused the opposite effect. This study provides evidence that Ang-1 plays a neuroprotective role by inhibiting of autophagy expression in SCIR injury. Overall, findings could be useful for the treatment of SCIR injury.

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Funding

Lianyungang Science and Technology Bureau provided financial support in the form of the Health and Family Planning Science and Technology Project (Grant No. 201703). The sponsor played no role in the design or conduct of this research.

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Author notes

  1. Jian Yin and Zhaoyang Yin have contributed equally to this work.

Authors and Affiliations

  1. Department of Orthopedics, The Affiliated Jiangning Hospital with Nanjing Medical University, Nanjing, 211100, People’s Republic of China

    Jian Yin, Bin Wang, Chao Zhu, Chao Sun & Xinhui Liu

  2. Department of Orthopedics, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People’s Hospital of Lianyungang, Lianyungang, 222000, People’s Republic of China

    Zhaoyang Yin

  3. Department of Geriatrics, Jinling Hospital, Medical School of Nanjing University, 211002, Nanjing, People’s Republic of China

    Ge Gong

Authors
  1. Jian Yin
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  2. Zhaoyang Yin
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  3. Bin Wang
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  4. Chao Zhu
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  5. Chao Sun
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  6. Xinhui Liu
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  7. Ge Gong
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Correspondence to Xinhui Liu or Ge Gong.

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Yin, J., Yin, Z., Wang, B. et al. Angiopoietin-1 Protects Spinal Cord Ischemia and Reperfusion Injury by Inhibiting Autophagy in Rats. Neurochem Res 44, 2746–2754 (2019). https://doi.org/10.1007/s11064-019-02893-3

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  • DOI: https://doi.org/10.1007/s11064-019-02893-3

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