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Role of voltage-gated L-type calcium channel in the spermatozoa motility of Atlantic salmon (Salmo salar)

https://doi.org/10.1016/j.cbpa.2019.110633Get rights and content

Highlights

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    Sperm motility is linked to voltage-gated L-type calcium channel in S. salar.

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    S. salar sperm treated with verapamil decrease their motility.

Abstract

Ca2+ cations play a key role in the initiation of spermatozoa motility in Atlantic salmon (Salmo salar). In this study we assess the importance of the voltage-gated L-type calcium channels in the spermatozoa motility of Atlantic salmon by combined in vitro and in silico approaches. The results of this study showed that as in other fish species, voltage-gated L-type calcium channels are significant in the spermatozoa motility of Salmo salar. The in vitro assays showed that total and progressive motilities decrease significantly (****p < .001) when Salmo salar spermatozoa are treated with verapamil, which has its binding site in the pore of the voltage-gated L-type calcium channel according to the in silico analysis.

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Declaration of Competing Interest

The authors declare that there are no conflicts of interest.

Acknowledgements

This work was supported by the DI12-PEO1 DIUFRO, DIE14-0001 DIUFRO, FONDECYT (1180387) projects and the UFRO PhD scholarship.

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