IL-13 and IL-4, but not IL-5 nor IL-17A, induce hyperresponsiveness in isolated human small airways

Background

Specific inflammatory pathways are indicated to contribute to severe asthma, but their individual involvement in the development of airway hyperresponsiveness remains unexplored.

Objective

This experimental study in human small bronchi aimed to provide insight into which of the type 2 and type 17 cytokines cause hyperresponsiveness of airway smooth muscle.

Methods

Explanted small bronchi isolated from human lung tissue and human airway smooth muscle cells were treated for 2 and 1 day(s), respectively, with 100 ng/mL of IL-4, IL-5, IL-13, or IL-17A, and contractile responses, Ca²⁺ mobilization, and receptor expression were assessed.

Results

Treatment with IL-13 increased the potency of histamine, carbachol, and leukotriene D₄ as contractile agonists. IL-4, but not IL-5 or IL-17A, also increased the potency of histamine. In human airway smooth muscle cells, IL-13 and IL-4, but not IL-5 and IL-17A, enhanced the histamine-induced Ca²⁺ mobilization that was accompanied with increased mRNA expression of histamine H₁ and cysteinyl leukotriene CysLT₁ receptors. RNA sequencing of isolated bronchi confirmed the IL-13–mediated upregulation of H₁ and CysLT₁ receptors, without showing an alteration of muscarinic M₃ receptors. Dexamethasone had no effects on IL-13–induced hyperresponsiveness in human bronchi, the increased Ca²⁺ mobilization, or the enhanced receptor expression. In contrast, antagonism of the common receptor for IL-13 and IL-4 by the biologic dupilumab prevented the effects of both IL-13 and IL-4 in human bronchi and human airway smooth muscle cells.

Conclusions

The glucocorticoid-insensitive hyperrresponsiveness in isolated human airways induced by IL-13 and IL-4 provides further evidence that the IL-4Rα pathway should be targeted as a new strategy for the treatment of airway hyperresponsiveness in asthma.