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Golgi-localized cyclophilin 21 proteins negatively regulate ABA signalling via the peptidyl prolyl isomerase activity during early seedling development

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Plant possesses particular Golgi-resident cyclophilin 21 proteins (CYP21s) and the catalytic isomerase activities have a negative effect on ABA signalling gene expression during early seedling development.

Abstract

Cyclophilins (CYPs) are essential for diverse cellular process, as these catalyse a rate-limiting step in protein folding. Although Golgi proteomics in Arabidopsis thaliana suggests the existence of several CYPs in the Golgi apparatus, only one putative Golgi-resident CYP protein has been reported in rice (Oryza sativa L.; OsCYP21-4). Here, we identified the Golgi-resident CYP21 family genes and analysed their molecular characteristics in Arabidopsis and rice. The CYP family genes (CYP21-1, CYP21-2, CYP21-3, and CYP21-4) are plant-specific, and their appearance and copy numbers differ among plant species. CYP21-1 and CYP21-4 are common to all angiosperms, whereas CYP21-2 and CYP21-3 evolved in the Malvidae subclass. Furthermore, all CYP21 proteins localize to cis-Golgi, trans-Golgi or both cis- and trans-Golgi membranes in plant cells. Additionally, based on the structure, enzymatic function, and topological orientation in Golgi membranes, CYP21 proteins are divided into two groups. Genetic analysis revealed that Group I proteins (CYP21-1 and CYP21-2) exhibit peptidyl prolyl cis–trans isomerase (PPIase) activity and regulate seed germination and seedling growth and development by affecting the expression levels of abscisic acid signalling genes. Thus, we identified the Golgi-resident CYPs and demonstrated that their PPIase activities are required for early seedling growth and development in higher plants.

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Acknowledgements

We thank Dr. An Gynheung (Kyung Hee University, Korea) for kindly providing us with the T-DNA insertion mutant of OsCYP21-1. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (Grant No. 2019R1A2C2002295) and Korea Research Institute of Bioscience and Biotechnology Research Initiative Programs (Grant Nos. KGM5371911 and KGM9481913) to H.S.C.

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  1. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, South Korea

    Haemyeong Jung, Seung Hee Jo, Hyun Ji Park, Areum Lee, Hyun-Soon Kim, Hyo-Jun Lee & Hye Sun Cho

  2. Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, South Korea

    Haemyeong Jung, Seung Hee Jo, Areum Lee & Hye Sun Cho

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  1. Haemyeong Jung
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Contributions

HSC designed the research. HJ performed the most of experiments. SHJ performed a part of qRT-PCR analysis. HJP and AL helped in cell biology experiments. H-SK and H-JL contributed to the biological interpretation. HJ and HSC analysed all data and wrote the paper.

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Correspondence to Hye Sun Cho.

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Jung, H., Jo, S.H., Park, H.J. et al. Golgi-localized cyclophilin 21 proteins negatively regulate ABA signalling via the peptidyl prolyl isomerase activity during early seedling development. Plant Mol Biol 102, 19–38 (2020). https://doi.org/10.1007/s11103-019-00928-5

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