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Effect of hydrogen-rich water on the Nrf2/ARE signaling pathway in rats with myocardial ischemia-reperfusion injury

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Abstract

The effects of hydrogen-rich water on oxidative stress via the Nrf2/ARE signaling pathway were studied in rats with myocardial ischemia-reperfusion injury (MIRI). Sixty rats were randomly divided into a hydrogen-rich water group and a control group, with 30 rats in each group. The two groups were randomly divided into three groups: pre-ischemic period, ischemic period and reperfusion period. After the heart was removed, it was fixed in a Langendorff device and perfused with an oxygen-balanced 37 °C perfusate. The control group was perfused with Kreb’s-Ringers (K-R) solution, and the hydrogen-rich water group was perfused with K-R solution + hydrogen-rich water. The levels of mRNA and protein of Nrf2, NQO1, HO-1 and SOD-1 in cardiomyocytes were detected by RT-qPCR, immunohistochemistry (IHC) and Western blot analysis. SOD activity and MDA content were determined. Hydrogen-rich water increased the activation of the Nrf2/ARE signaling pathway, and the levels of mRNA and protein Nrf2, NQO1, HO-1 and SOD-1 were significantly increased (P < 0.05) in the ischemia-reperfusion period compared with the ischemic period. In the control group, the levels of mRNA and protein of Nrf2, NQO1, HO-1 and SOD-1 were significantly decreased (P < 0.05) in the ischemia-reperfusion period compared with the ischemic period. Compared with the ischemic period, the ischemia-reperfusion phase showed significantly increased SOD activity and significantly decreased MDA content in the hydrogen-rich water group, while SOD activity was significantly decreased, and MDA content was significantly increased in the control group (P < 0.05). Hydrogen-rich water can activate the Nrf2/ARE signaling pathway, alleviate ischemia-reperfusion injury in isolated rat hearts and reduce the oxidative stress level of myocardial tissue.

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All data generated or analyzed during the present study are included in this published article.

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Acknowledgements

The authors are grateful for the help provided by the Affiliated Hospital of Hebei University of China. The authors are grateful for the experimental hydrogen-rich water provided by Mr. Zhilin Li from the College of Chemistry, Hebei University (patent number ZL102557227B).

Funding

This research was financially supported by the Medical Science Research Key Project Foundation of Hebei Province (No. 20130369).

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Author notes

  1. Liangtong Li and Tongtong Liu contributed equally to this work and should be regarded as co-first authors.

Authors and Affiliations

  1. Medical College, Hebei University, Baoding, 071000, China

    Liangtong Li, Li Liu, Shaochun Li, Zhe Zhang, Ruisha Zhang & Yujuan Zhou

  2. Affiliated Hospital of Hebei University, Baoding, 071000, China

    Tongtong Liu & Fulin Liu

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  1. Liangtong Li
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Contributions

Liangtong Li, Tongtong Liu, Li Liu, Shaochun Li and Zhe Zhang performed the qRT-PCR, Western blot and immunohistochemical analyses. Liangtong Li and Tongtong Liu collected the data and performed the statistical analyses. Zhe Zhang and Ruisha Zhang participated in sample collection and in the detection of SOD activity and MDA content. Yujuan Zhou and Fulin Liu designed the study. Liangtong Li and Tongtong Liu wrote the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Yujuan Zhou or Fulin Liu.

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The present study was performed at the Central Laboratory of Affiliated Hospital of Hebei University. All animal experiments were approved by the Animal Ethical and Welfare Committee of Hebei University (Baoding, China, approval no. 2017010) and performed in accordance with the Guidelines for the Care and Use of Laboratory Animals of Hebei University.

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Li, L., Liu, T., Liu, L. et al. Effect of hydrogen-rich water on the Nrf2/ARE signaling pathway in rats with myocardial ischemia-reperfusion injury. J Bioenerg Biomembr 51, 393–402 (2019). https://doi.org/10.1007/s10863-019-09814-7

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