Cell Host & Microbe
Volume 26, Issue 6, 11 December 2019, Pages 764-778.e5
Journal home page for Cell Host & Microbe

Article
Whole-Virome Analysis Sheds Light on Viral Dark Matter in Inflammatory Bowel Disease

https://doi.org/10.1016/j.chom.2019.10.009Get rights and content
Under an Elsevier user license
open archive

Highlights

  • Analyzing viral dark matter changes our understanding of the gut virome

  • Gene-based clustering can address the challenges of high inter-individual variation

  • Healthy human gut virome is dominated by a stable core of virulent bacteriophages

  • In Crohn’s disease, the virulent core is replaced with temperate bacteriophages

Summary

The human gut virome is thought to significantly impact the microbiome and human health. However, most virome analyses have been performed on a limited fraction of known viruses. Using whole-virome analysis on a published keystone inflammatory bowel disease (IBD) cohort and an in-house ulcerative colitis dataset, we shed light on the composition of the human gut virome in IBD beyond this identifiable minority. We observe IBD-specific changes to the virome and increased numbers of temperate phage sequences in individuals with Crohn’s disease. Unlike prior database-dependent methods, no changes in viral richness were observed. Among IBD subjects, the changes in virome composition reflected alterations in bacterial composition. Furthermore, incorporating both bacteriome and virome composition offered greater classification power between health and disease. This approach to analyzing whole virome across cohorts highlights significant IBD signals, which may be crucial for developing future biomarkers and therapeutics.

Keywords

virome
phageome
inflammatory bowel disease
IBD
CD
UC
ulcerative colitis
Crohn’s disease
phage
virus
16S

Cited by (0)

3

These authors contributed equally

4

Present address: South Australian Health and Medical Research Institute, Adelaide, Australia

5

Present address: Synlogic, Cambridge, MA, USA

6

Lead Contact