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Overexpression LINC01082 suppresses the proliferation, migration and invasion of colon cancer

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Abstract

Long non-coding RNAs (lncRNAs) are emerging as pivotal regulators in human cancer. LINC01082 was expressed as decreased in colon cancer by previous lncRNA-seq result and TCGA database, however, the role and function of LINC0182 is not clear in colon cancer. Here, we aimed to explore the role of LINC01082 in colon cancer for exploring the etiopathogenesis of colon cancer. RT-qPCR for LINC01082 expression in tissues (colon cancer vs. their matched adjacent non-cancerous tissues, ANT, n = 39) and cells (colon cancer cells vs. normal colon cells, n = 4) were performed. CCK-8 assay for proliferation of colon cancer, Transwell assay for migration and invasion were carried out in sw480 and sw620 cells. The results revealed that LINC01082 was significantly decreased in tissues and cell lines of colon cancer. Overexpressed LINC01082 significantly suppressed the proliferation ability of colon cancer cells. The migration and invasion of colon cancer cells were also suppressed after LINC01082 overexpression. These findings demonstrated that LINC01082 may act in suppressing the incidence and development of colon cancer via suppressing cell proliferation, migration and invasion, indicating that LINC01082 may act as a new tumor suppressor and may be a promising therapy target for colon cancer.

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Data availability

The datasets used during the present study are available from the corresponding author upon reasonable request.

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Funding

This work was supported by the Training Program for Medical Reserve Talents of the Health and Family Planning Commission of Yunnan Province [Grant Number H-201641].

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Author notes

  1. Wei Xiong, Jiyong Qin, Xinyi Cai, and Wei Xiong have contributed equally to this work.

Authors and Affiliations

  1. Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, 519 Kunzhou Road, Xishan District, Kunming, 650100, Yunnan, People’s Republic of China

    Wei Xiong, Jiyong Qin, Qiuyan Liu, Cheng Li, Yunhe Ju, Qiaoli Wang & Yi Yang

  2. Department of Colorectal Surgery, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, 519 Kunzhou Road, Xishan District, Kunming, 650100, Yunnan, People’s Republic of China

    Xinyi Cai, Wei Xiong & Yunfeng Li

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  1. Wei Xiong
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  2. Jiyong Qin
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  4. Wei Xiong
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  10. Yi Yang
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Contributions

WX, JQ, and XC conceived and designed the study. WX, JQ, XC, WX, QL, CL, YJ, and QW performed the experiments. WX wrote the paper. YL and YY reviewed and edited the manuscript. All authors read and approved the manuscript and agreed to be accountable for all aspects of the research in ensuring that the accuracy or integrity of any part of the work is appropriately investigated and resolved.

Corresponding authors

Correspondence to Yunfeng Li or Yi Yang.

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The authors declare that they have no competing interests.

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All experimental protocols were approved by the human Ethics Committee of Kunming Medical University. The written informed consent was obtained from all patients.

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Xiong, W., Qin, J., Cai, X. et al. Overexpression LINC01082 suppresses the proliferation, migration and invasion of colon cancer. Mol Cell Biochem 462, 33–40 (2019). https://doi.org/10.1007/s11010-019-03607-7

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  • DOI: https://doi.org/10.1007/s11010-019-03607-7

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