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Vaspin protects mouse mesenchymal stem cells from oxidative stress-induced apoptosis through the MAPK/p38 pathway

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Abstract

The aim of the work was to study the influence of vaspin on oxidative stress-induced apoptosis of mouse mesenchymal stem cells (MSCs). MSCs originated from bone marrow of C57BL/6 mouse were treated with vaspin and/or H2O2 in a dose-dependent manner. Cellular viability detected by CCK-8 and cell apoptosis studied by flow cytometry and TUNEL assay were observed in these cells. The protein expressions of PI3K, p-PI3K, Akt, p-Akt, T-ERK1/2, p-ERK1/2, p38, p-p38, JNK, and p-JNK were tested by Western blot. Vaspin had no significant effect on cellular viability, but significantly reduced H2O2-induced apoptosis. Western blot assay showed that pretreatment with vaspin promoted the activation of p-p38. Inhibition of p38 by SB203580 suppressed the protective effect of vaspin on oxidative stress-induced apoptosis. Vaspin inhibits oxidative stress-induced apoptosis of MSCs via the activation of MAPK/p38 signaling pathway. These findings indicate that vaspin is prone to osteoporosis protection.

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Funding

This study was funded by the National Natural Science Foundation of China (Grant No. 81400849); the Natural Science Foundation of Guangdong Province (Grant No. 2014A030310490); the Science and Technology Planning Project of Guangdong Province, China (Grant No. 2017A020215189); the Science and Technology Planning Project of Tianhe District, Guangdong Province, China (Grant No. 2013kw004).

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    1. Department of Endocrinology and Metabolism, The Third Affiliated Hospital of Southern Medical University, No. 183 Tianhe District, Guangzhou, 510630, Guangdong Province, China

      Xiao Zhu, Jicheng Lv, Shidi Hu & Jie Shen

    2. Department of Medical Image, The Third Affiliated Hospital of Southern Medical University, Guangzhou, 510630, Guangdong Province, China

      Lingyan Zhang

    3. Department of Medical Laboratory, The Third Affiliated Hospital of Southern Medical University, Guangzhou, 510630, Guangdong Province, China

      Youming Chen

    4. Department of Endocrinology, Guangdong Second Provincial Hospital, Guangzhou, 510317, Guangdong Province, China

      Bo Chen

    5. Department of Internal Medicine, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510120, Guangdong Province, China

      Haifeng Huang

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    Zhu, X., Zhang, L., Chen, Y. et al. Vaspin protects mouse mesenchymal stem cells from oxidative stress-induced apoptosis through the MAPK/p38 pathway. Mol Cell Biochem 462, 107–114 (2019). https://doi.org/10.1007/s11010-019-03614-8

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