Correction to: Nature Protocols https://doi.org/10.1038/s41596-019-0179-x, published online 26 June 2019

In the version of this paper originally published, step 10 in Box 1 incorrectly stated that the user should carry out 40 cycles of each of three temperature and time stages in the PCR run. The run should actually contain only two steps, with the second including a shift from 95 °C to 60 °C. The instructions have been corrected in the PDF and HTML versions of the protocol.