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Studying the Effect of Ionizing Radiation on Radiation-Induced Changes in the Cells of the Blood System in Mice at the Organism Level

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Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract

The task of studying radiation “bystander effects” at the organism level was set in the work. Irradiated and nonirradiated mice that were kept together were used in the experiment. Mice were irradiated at a dose of 3 Gy in a research radiobiological gamma installation with the sources of 137Cs. The number of leukocytes and relative number of lymphocytes in the peripheral blood of mice were estimated 3, 7, 14, 30, 60, and 90 days after the beginning of the experiment. In nonirradiated “bystander” mice kept with irradiated mice both in the cages without a partition and with a partition, there is a possible trend towards a decrease in the number of leukocytes as compared with indices in the biocontrol. During the analysis of relative number of lymphocytes, a trend towards a decrease in the index in “bystander” mice was demonstrated both when keeping without a partition and with a partition. In nonirradiated “bystander” animals when keeping with irradiated animals in the cage with a partition, a statistically significant decrease in the relative number of lymphocytes in the peripheral blood was registered on days 3 (t = 2.13; p = 0.047), 30 (t = 2.94; p = 0.01), and 90 (t = 3.07; p = 0.01) after irradiation as well as when keeping in the same cage with irradiated mice without a partition on day 60 (t = 2.24; p = 0.038) after the beginning of the experiment. A “bystander effect” in nonirradiated animals is one of possible explanations for the detected changes. In irradiated animals that were kept in the same cage with a partition together with nonirradiated animals, a statistically significant increase in the relative number of lymphocytes was registered on days 3 (t = 2.6; p = 0.02), 14 (t = 2.61; p = 0.018), and 60 (t = 2.38; p = 0.03) (“rescue effect”). Based on data obtained in the present experiment, it is assumed that the radiation “bystander effect” can have the opposite nature; that is, nonirradiated organisms are able to reduce the radiation effects in irradiated individuals (“rescue effect”).

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Funding

This work was carried out within the state task of the Ministry of Science and Higher Education of the Russian Federation (registration number no. 122040400089-6) due to a subsidy allocated to the Federal Research Center for Chemical Physics, Russian Academy of Sciences, for the implementation of the state task, topic: “Study of the Principles of Structural and Functional Organization of Biomolecular Systems, Development of the Methods for the Design of Their Physicochemical Analogues and Creating a New Generation of Biologically Active Preparations on This Basis.”

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Authors and Affiliations

  1. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, Moscow, 119991, Russia

    I. N. Kogarko, V. V. Petushkova, B. S. Kogarko, E. A. Neyfakh, O. V. Ktitorova, I. I. Ganeev, N. S. Kuzmina, E. I. Selivanova & I. I. Pelevina

  2. Ural Research Center for Radiation Medicine, Federal Medical-Biological Agency of Russia, Chelyabinsk, 454076, Russia

    E. A. Pryakhin & S. S. Andreev

  3. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 117971, Russia

    N. S. Kuzmina

  4. Tsyb Medical Radiological Research Center, National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Obninsk, 249036, Russia

    E. I. Selivanova

Authors
  1. I. N. Kogarko
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  2. V. V. Petushkova
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  3. B. S. Kogarko
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  4. E. A. Pryakhin
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  5. E. A. Neyfakh
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  6. O. V. Ktitorova
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  7. S. S. Andreev
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  8. I. I. Ganeev
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  9. N. S. Kuzmina
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  10. E. I. Selivanova
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  11. I. I. Pelevina
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Corresponding author

Correspondence to V. V. Petushkova.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Translated by A. Barkhash

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Kogarko, I.N., Petushkova, V.V., Kogarko, B.S. et al. Studying the Effect of Ionizing Radiation on Radiation-Induced Changes in the Cells of the Blood System in Mice at the Organism Level. Biochem. Moscow Suppl. Ser. B 17, 145–151 (2023). https://doi.org/10.1134/S1990750823600292

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