A sensitive and recyclable substrate was fabricated through in situ reduction of silver nanoparticles (NPs) on zinc oxide nanorods (NRs). The prepared silver nanoparticles/zinc oxide nanorods/polyamide mesh (Ag/ZnO/PM) substrate exhibited not only excellent surface-enhanced Raman scattering (SERS) performance to R6G with a limit of detection (LOD) of 10⁻¹² M, mainly attributed to the synergistic effect of the suitable size and the nanoscale gaps of the Ag NPs to produce local surface plasmon resonance (LSPR), but also outstanding self-cleaning property via UV irradiation due to its significant photocatalytic property based on the non-equilibrium carriers generated by ZnO and the presence of Schottky junctions between Ag and ZnO. The substrate showed good recycling stability even after five cycles. Furthermore, the successful recyclable application of Ag/ZnO/PM for tetracycline hydrochloride (TC) detection with high sensitivity further suggested that it is a promising candidate for constructing a portable SERS platform to detect organic pollutants.