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Isolation, Purification, and Properties of Glycoprotein from Sheep Intestinal Mucous Membrane

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Chemistry of Natural Compounds Aims and scope

A glycoprotein inhibiting the growth of colon cancer cells was isolated from sheep intestinal mucous membrane. The glycoprotein, designated IGP, was purified by a combination of ion-exchange chromatography over DEAE-650M and gel-filtration over HW-55F. Purified IGP had a molecular mass of ~60 kDa and consisted of 68.72 ± 2.10% protein and 32.09 ± 1.52% carbohydrates. The monosaccharide composition of the carbohydrate part consisted of glucose (31.28% of the total carbohydrate content), glucosamine (22.28%) and N-acetylgalactosamine (19.38%), mannose (11.14%), fucose (11.28%), and rhamnose (4.64%). The amino-acid composition of IGP was also determined.

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Acknowledgment

The work was supported by grants of Xinjiang-Uyghur Autonomous District “Tianshan Yingcai-Leading Talents in Scientific and Technological Innovation (No. 2022TSYCLJ0008) and Tianshan Cedar Plan – Science and Technology Innovation Leaders Reserve Candidate Program (No. 2020XS08). We are also grateful to Xinjiang Biochemical Pharmaceutical Co., Ltd., for technical support of the work.

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Authors and Affiliations

  1. State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization and Key Laboratory of Chemistry of Plant Resources in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40-1, 830011, Urumqi, P. R. China

    Y. H. Wang, G. Turdu, A. L. Hu, Z. Yang, N. Dongmulati, A. Arken, R. Kelaimu, A. Wali, Y. Ali, Y. H. Gao, A. Yili & H. A. Aisa

  2. University of Chinese Academy of Science, 100039, Beijing, P. R. China

    Y. H. Wang, G. Turdu, A. L. Hu, Z. Yang, N. Dongmulati, A. Arken, R. Kelaimu & Y. Ali

  3. Xinjiang Biochemical Pharmaceutical Co., Ltd, 830032, Urumqi, P. R. China

    X. M. Zhao

  4. A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, 100125, Republic of Uzbekistan

    G. T. Mavlonov

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  1. Y. H. Wang
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  2. G. Turdu
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  9. A. Wali
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  13. A. Yili
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  14. H. A. Aisa
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Corresponding author

Correspondence to A. Yili.

Additional information

Translated from Khimiya Prirodnykh Soedinenii, No. 1, January–February, 2024, pp. 118–121.

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Wang, Y.H., Turdu, G., Zhao, X.M. et al. Isolation, Purification, and Properties of Glycoprotein from Sheep Intestinal Mucous Membrane. Chem Nat Compd 60, 138–142 (2024). https://doi.org/10.1007/s10600-024-04268-9

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  • DOI: https://doi.org/10.1007/s10600-024-04268-9

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