Polarized branched Actin modulates cortical mechanics to produce unequal-size daughters during asymmetric division

Daeden, Alicia; Mietke, Alexander; Derivery, Emmanuel; Seum, Carole; Jülicher, Frank; Gonzalez-Gaitan, Marcos

doi:10.1038/s41556-022-01058-9

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Published: 06 February 2023

Polarized branched Actin modulates cortical mechanics to produce unequal-size daughters during asymmetric division

Nature Cell Biology volume 25, pages 235–245 (2023)Cite this article

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Abstract

The control of cell shape during cytokinesis requires a precise regulation of mechanical properties of the cell cortex. Only few studies have addressed the mechanisms underlying the robust production of unequal-sized daughters during asymmetric cell division. Here we report that unequal daughter-cell sizes resulting from asymmetric sensory organ precursor divisions in Drosophila are controlled by the relative amount of cortical branched Actin between the two cell poles. We demonstrate this by mistargeting the machinery for branched Actin dynamics using nanobodies and optogenetics. We can thereby engineer the cell shape with temporal precision and thus the daughter-cell size at different stages of cytokinesis. Most strikingly, inverting cortical Actin asymmetry causes an inversion of daughter-cell sizes. Our findings uncover the physical mechanism by which the sensory organ precursor mother cell controls relative daughter-cell size: polarized cortical Actin modulates the cortical bending rigidity to set the cell surface curvature, stabilize the division and ultimately lead to unequal daughter-cell size.

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Main

Many asymmetric cell divisions (ACDs) are characterized by a difference in size of the two daughter cells¹. From a physical point of view, the generation of unequal daughter-cell size represents a mechanical challenge during cytokinesis. According to Laplace law, if two daughter cells are not identical in size, the smaller daughter would collapse into the bigger daughter during cytokinesis. Indeed, cells, dividing under a global contractile tension at the poles, are expected to exhibit dramatic shape instabilities^2,3. As daughter cells are never exactly identical, cells must ensure to compensate for such instabilities, which can ultimately lead to cytokinetic failure². Consequently, the precise control of the mechanical properties at the cortical poles is critical during ACD, when cells divide into unequal daughter-cell size. Identifying the mechanisms of daughter-cell size control is also crucial to understand cell function, because the ability to produce asymmetric daughters underlies cellular diversity and can influence proliferation rates⁴ and cell differentiation^4,5,6, which are key for stem cell renewal^7,8,9.

Previous reports have proposed a role of spindle positioning in determining size asymmetry^10,11,12. However, recent data suggest that spindle-induced cleavage furrow positioning is not enough to explain cell-size asymmetry (reviewed in ref. ¹³) and cells without spindles can divide with normal daughter-cell size asymmetry^14,15. Furthermore, this does not resolve how the cell overcomes the mechanical instabilities during cytokinesis mentioned above.

Instead, the actomyosin cortex at the cell poles has been proposed to play a key role in the generation of cell size asymmetry^5,14,15. These previous studies have mainly focused on the role of contractile actomyosin tension in determining cortical mechanical properties, while the role of other mechanical properties, such as cortical stiffness or bending rigidity, is less well understood. For example, during asymmetric neuroblast division in Caenorhabditis elegans and Drosophila, cortical Myosin enrichment in the smaller pole has been proposed to produce unequal-sized daughters by creating an asymmetry of contractile tension^5,14,15. However, during these divisions, it is unknown how shape instabilities, such as the collapse of the smaller cell pole, which exhibits a larger contractile tension, are prevented². Thus, how cells control and stabilize their shape and relative daughter-cell size during ACD remains an open question. In this Article, we investigate how polar branched Actin at the cell cortex regulates cortical mechanics to robustly achieve asymmetric division into two unequal-sized daughter cells.

Results

Daughter-cell size asymmetry during SOP division

Sensory organ precursor (SOP) cells divide into an anterior PIIB and a posterior PIIA daughter cell. To study the sizes of PIIA and PIIB (Fig. 1a), we analysed the three-dimensional (3D) geometry of dividing SOPs from imaging data (Fig. 1b and Supplementary Note 1). During cytokinesis, SOP volume remains constant, and, at the end of mitosis, PIIA has nearly twice the volume of PIIB (Fig. 1c,d and Extended Data Fig. 1a–c). The total surface area increases by 20%, as expected for a sphere when it is split into two with volume conservation (Fig. 1e and Supplementary Information II.I). Using the cleavage furrow as landmark, we found that the increase of total surface area is mainly due to an increase in posterior pole surface (Fig. 1e). To identify the origin of this surface area asymmetry and corresponding cell size asymmetry, we studied the cortical cytoskeleton at the poles.

**Fig. 1: Daughter-cell size and cortical Actin asymmetry.**

Cortical Actin asymmetry during asymmetric mitosis

As in other mitotic systems^16,17, Actin is recruited to the SOP cortex at metaphase onset (Extended Data Fig. 1d) during the mitotic rounding process^18,19,20. In contrast, in late anaphase, the posterior Actin cortex is enriched by fourfold (Fig. 1f,g, Supplementary Video 1 and Extended Data Fig. 1d–h) and is 40% thicker compared with the anterior cortex (Extended Data Fig. 1i,j). In accordance with this, Actin dynamics in the posterior cortex is slower, as revealed by fluorescence recovery after photobleaching (FRAP) using green fluorescent protein (GFP)-Actin (Extended Data Fig. 1k,l). Similar to the SOP, Drosophila larval neuroblasts also display asymmetric cortical Actin enrichment, with higher levels in the apical pole, which gives rise to the neural stem cell, the larger daughter (Extended Data Fig. 2a,b).

Formins and the Arp2/3 complex drive the polymerization of linear and branched filamentous Actin (F-Actin), respectively^21,22,23. In SOPs, we show that asymmetric cortical F-Actin probably arises from an asymmetric Arp2/3 activation. Indeed, when Nausicaa, a protein known to regulate branching nucleation and density in Drosophila²⁴, is downregulated, Actin asymmetry is abolished (Fig. 1g and Extended Data Fig. 2c,d). Conversely, when Formins are inhibited by the SMIFH2 drug^25,26,27, cortical Actin asymmetry is not affected (Extended Data Fig. 2e,f). Furthermore, both Rac and Cdc42, upstream regulators of Arp2/3 (ref. ²⁸), are enriched in the posterior cortex (Extended Data Fig. 3a–d), but not Diaphanous, which is the main Formin known to nucleate Actin at the cell cortex and to be involved during cytokinesis^21,29,30,31 (Extended Data Fig. 3e,f). Additionally, only Filamin (a large, flexible crosslinker arranging Actin into a meshwork)^32,33 is found enriched at the cortical poles, but not Fascin, α-Actinin or Fimbrin, all of which organize F-Actin into bundles^34,35,36 (Extended Data Figs. 3g–j and 4a,b). Therefore, Actin asymmetry seems to be dominated by branched Actin in SOPs.

In other ACDs, enrichment of cortical Myosin has been shown to correlate with the smaller daughter cell^5,14,15. We then set up to study and manipulate contractility factors (such as Myosin and bundler proteins) in SOPs (Extended Data Fig. 4a–l). For instance, Myosin-II is found enriched at the cell cortex, but no asymmetry could be detected (Extended Data Fig. 4c–e). Therefore, asymmetric cell size in SOPs might arise from an alternative mechanism, where the asymmetric accumulation of branched cortical Actin, instead of Myosin, determines relative daughter-cell sizes. To investigate this, we next studied the functional relationship between cortical Actin asymmetry and unequal daughter-cell size.

Actin asymmetry coupled with PAR complex and daughter size

Cell size asymmetry during ACD can be controlled through polarity cues organized by the PAR complex³⁷. We thus studied cortical Actin in SOPs expressing a phospho-defective lgl mutant version (lgl^3A) known to impair PAR complex activity³⁸.

While in control SOPs the polarity marker Pon is restricted to the anterior cortex by the PAR complex³⁹ (Figs. 1 a and 2a), in lgl^3A mutants, Pon is found at the cell cortex of both poles³⁸ (Fig. 2a). Strikingly, in lgl^3A, SOPs divide into two daughters of similar size (Fig. 2b) and exhibit symmetric cortical Actin (Figs. 1g and 2c,d; see also Extended Data Fig. 5a–e for dsh and Gβ13F phenotypes). This indicates that the mechanism underlying the posterior accumulation of Actin is under the control of the PAR complex. To further study the relationship between Actin and size asymmetry, we next uncoupled Actin polarity from the position of the mitotic plane.

**Fig. 2: Cortical Actin asymmetry correlates with daughter-cell size asymmetry.**

Polar Actin asymmetry levels correlate with daughter size

During SOP mitosis, three features align to the anterior–posterior axis: (1) the posterior cortical PAR domain (excluding Pon to the anterior), (2) the mitotic spindle orientation and (3) the posterior cortical Actin domain. A Mud–Dynein complex couples spindle orientation to the PAR domain⁴⁰. Indeed, in mud⁴ mutants, the Pon domain is bisected in random locations by the cleavage plane^41,42,43 (Fig. 2e). In this condition, daughter-cell sizes display a broad range from being asymmetric as in wild type to symmetric daughters (Fig. 2b).

Like in control SOPs, the Actin and Pon domains in mud⁴ still exclude each other, confirming that Actin is enriched within the PAR domain (Fig. 2e,f), consistent with Par6/Cdc42 interactions as previously reported^44,45,46. Consequently, not only the Pon domain but also the Actin domain is randomly partitioned between the two poles in mud⁴ (Fig. 2e,f). This condition therefore generates a continuum of cortical Actin asymmetry levels that correlate with the degree of cell size asymmetries (Fig. 2g and Extended Data Fig. 5f): low levels of Actin asymmetry give rise to symmetric sizes, while posterior enrichment of Actin leads to wild-type-like asymmetric daughter-cell size. Therefore, the level of Actin asymmetry ultimately forecasts the relative sizes of the two daughters.

Nanobody mistargeting of Actin can invert size asymmetry

In mud⁴ experiments, the position of the PAR domain and the Actin domain remain correlated (Fig. 2e,f): PAR might control in parallel Actin asymmetry and size asymmetry, where size would be Actin independent. To study whether polar Actin can directly control size, we inverted the Actin asymmetry with nanobodies^47,48 while keeping the PAR polarity normal. We co-expressed in SOPs a GFP fusion of WAVE, the major cortical regulator of Arp2/3 (ref. ⁴⁹) (Extended Data Fig. 5g,h), and GFP-binding protein (GBP)-Pon, an anti-GFP nanobody fused to the localization domain of Pon. This way, GFP-tagged WAVE is targeted to the anterior cortex, causing an anterior cortical accumulation of branched F-Actin (Fig. 3a,b and Extended Data Fig. 5i,j) and daughter-cell size inversion: the anterior daughter becomes larger than the posterior (Fig. 3a,c and Supplementary Video 2). Interestingly, while, in lgl^3A mutant, daughter-cell size is symmetrical, targeting WAVE to one pole by optogenetics (see below for details) in lgl^3A mutant is sufficient to yield this pole larger and can thereby generate asymmetric daughter-cell size (Extended Data Fig. 5k,l).

**Fig. 3: Cortical Actin manipulation through polar nanobodies.**

In contrast, targeting Myosin, α-Actinin or Fimbrin (two crosslinkers generating F-Actin bundles^34,35,36) by nanobody targeting to the anterior pole leads to a smaller rather than a larger daughter cell (Extended Data Fig. 4f–l and Supplementary Information III.II.2.c). This indicates that the effect of branched Actin on the cell cortex mechanics is distinct from Myosin-mediated contractile tension (see below).

Conversely, we exacerbated Actin asymmetry beyond wild type by targeting WAVE-GFP to the posterior cortex through co-expression of GBP-Baz⁴⁸, a GBP fusion to the Drosophila Par3 orthologue Bazooka. This leads to a more extreme size asymmetry compared with control SOPs (Fig. 3c and Extended Data Fig. 5m,n). These nanobody WAVE experiments suggest that the relative daughter-cell sizes can be directly determined by the cortical Actin asymmetry. In wild type, PAR controls Actin asymmetry and Actin, in turn, determines unequal cell size.

Interestingly, in the inversion experiment in which PIIA becomes smaller than PIIB (Fig. 3a,c), we noticed that bristle length in the adult fly was also reduced (Fig. 3d,e). This bristle cell derives from the lineage of the large PIIA cell, opening the possibility that PIIA/PIIB size asymmetry impacts the final cell sizes in the subsequent lineage and thereby the functions of these mechanoreceptors.

Impairing cortical branched Actin at the cell poles

In the previous experiments, we enriched the cortical Actin in each pole. We next impaired the cortical Actin asymmetrically through targeting of Arpin, AIP1 or Cofilin. Arpin inhibits Arp2/3 (ref. ⁵⁰), while AIP1 or Cofilin sever Actin filaments⁵¹. Posterior targeting of these factors using GBP-Baz caused embryonic lethality precluding their study at the subsequent pupal stage. To overcome this, we established optogenetics for temporal control based on CRY2/CIB⁵², whose binding is induced by 445/488 nm laser light.

By co-expression of Lifeact-CRY2^FL-mCherry and CIB¹-GFP-Arpin in SOPs, we recruited Arpin to Actin-rich regions with spatio-temporal control (posterior cortex at anaphase onset) using holographic patterned photo-stimulation microscopy⁵³. Under these conditions, the relative Actin asymmetry between the two poles is lost (Fig. 4a–f and Supplementary Video 3) and daughter-cell sizes become symmetrical (Fig. 4g). As expected, when Arpin is optogenetically targeted to both poles, only the general levels of Actin density are affected and the effect on size asymmetry is observed to a lesser extent (Extended Data Fig. 6a–e). Furthermore, when size asymmetry is abolished by impairing branched F-Actin nucleation at the posterior cortex, cell polarity and, in particular, asymmetric spindle positioning are not affected (Extended Data Fig. 6f–h). This suggests that asymmetric spindle positioning by itself is not enough to drive size asymmetry (see below).

**Fig. 4: Impairing cortical branched Actin at the posterior pole through optogenetics leads to symmetric daughters.**

Conversely, impairing the cortical Actin meshwork only in the anterior pole by using GBP-Pon and GFP fusions to Arpin, AIP1 or Cofilin led to a small but notable enhancement of daughter size asymmetry (Extended Data Fig. 7a–h). Furthermore, while inhibiting Formins does not affect size asymmetry, downregulating branched Actin nucleation leads to symmetrical daughters (Fig. 2b and Extended Data Figs. 2c–f and 5e), confirming that it is branched Actin that could modulate the daughter-cell sizes.

Taken together, our results confirm that the relative amount of branched Actin between the two cortices and, in particular, posterior branched Actin enrichment are key to producing size asymmetry.

Polar Actin acts beyond spindle positioning

Cortical actomyosin is known to influence mitotic spindle positioning⁵⁴. The antiparallel microtubule overlap established in metaphase positions the centralspindlin complex, which itself organizes the actomyosin ring in anaphase^55,56 and, hence, the cytokinetic furrow^2,57. This could, in turn, determine daughter-cell size. In SOPs, the metaphase plate is asymmetrically positioned towards the anterior pole (Extended Data Fig. 8a–f), yet this asymmetry is not enough to explain the asymmetry of size observed in wild type (Supplementary Information II.II). Furthermore, SOPs treated with Colcemid, to depolymerize all microtubules, still generate two poles with a size asymmetry similar to wild-type SOPs (Extended Data Fig. 8g–i), as previously reported in Drosophila neuroblasts^14,15. This further supports the fact that asymmetric spindle positioning does not dominate unequal cell size in SOPs. Nonetheless, this prompted us to ask whether the inversion of daughter-cell size observed during the anterior WAVE targeting could be influenced by an Actin-mediated spindle positioning process during metaphase or whether it was only dependent on the polar Actin cortex having an effect on cortical mechanics.

To test this, we used LOV/PDZ optogenetics, where blue illumination opens the LOV domain to expose a PDZ binding domain⁵⁸, at different stages of cytokinesis. We co-expressed three proteins: WAVE-GFP, GBP-LOV and PDZ-Pon (Fig. 5a–d). WAVE-GFP binds GBP-LOV and, upon blue illumination, is targeted to PDZ-Pon in the anterior cortex (Fig. 5b–d). Photoactivation at any timepoint from metaphase to late anaphase led to cell size inversion through Actin enrichment in the anterior cortex (Fig. 5b–d and Supplementary Video 4). Importantly, in late anaphase, the positions of the spindle midzone and the actomyosin ring are already committed (Extended Data Fig. 8e,f). Yet, photoactivation in late anaphase still affected daughter size asymmetry (Fig. 5b). Therefore, polar cortical Actin itself can control daughter size asymmetry independently of spindle positioning and relative daughter-cell sizes can still be modified until late stages of cytokinesis. This is consistent with the possibility that cortical Actin directly controls cell size by regulating the material properties and mechanics of the polar cortex itself.

**Fig. 5: Late anaphase manipulation of cortical Actin leads to daughter-cell size inversion.**

Indeed, mechanical properties between the two poles are different. Blebs are local reporters of cortical tension: their size correlates indeed with cortical tension⁵⁹. At the end of SOP mitosis, blebs are more frequent and larger in the posterior pole (Extended Data Figs. 1e and 9a,b). We also confirmed this cortical tension asymmetry by generating laser-induced blebs by ablation of the cell cortex (Extended Data Fig. 9c,d).

Physics of daughter-cell size asymmetry

We then established a minimal physical model of cortical mechanics to study (1) which mechanical factors, modulated by Actin, are required for asymmetric size, (2) whether differences in cortical Actin asymmetry levels can explain quantitatively the experimental size asymmetries observed and (3) under which conditions are daughter-cell shape geometries mechanically stable. Following experimental observations (Extended Data Fig. 9e,f), our model neglects effects from neighbouring cells.

We considered a minimal model where a spherical cell of radius R divides into two daughter cells, represented by two connected spherical caps⁶⁰ (Fig. 6a and Extended Data Fig. 9g) that can have different mechanical surface properties (Supplementary Information III): a contractile cortical tension σ, a bending rigidity κ (cortical stiffness in response to bending deformations) and a cortical spontaneous curvature C₀ (intrinsic tendency to bend). The cortical actomyosin cytoskeleton could modulate these material properties^61,62, thereby contributing to cortical mechanical responses, cortical curvature and, ultimately, relative daughter sizes.

**Fig. 6: Physics of cell size asymmetry: branched Actin asymmetry controls daughter-cell size through curvature.**

We explored a mechanism where κ and σ are determined by the cortical Actin density I. For simplicity, both κ and σ increase linearly with I, according to κ = α_κI and σ = α_σI (Supplementary Information III.II.3). In this model, different Actin asymmetry levels between the two caps, for given α_κ, α_σ and C₀ values, give rise to different force-balanced surface geometries characterized by a size asymmetry ∆a (Fig. 6a). Consequently, we fit our model to the experimentally measured, Actin-dependent PIIA/PIIB cell size asymmetries (R² = 0.96; Fig. 6b,c and Extended Data Fig. 9h).

For the fit, only two free parameters need to be determined: (1) the ratio α_κ/α_σ and (2) C₀ (relative to cell size; Supplementary Information III.II.3). With these two parameters alone, we can quantitatively explain the cell size ratios observed in our experiments, from the asymmetric wild-type scenario and the symmetric mutant conditions to those in which the size ratios are inverted (Fig. 6b,c). Importantly, the fairly large value of $\frac{{\alpha _\kappa }}{{\alpha _\sigma R^2}} = 7.1 \pm 0.8$ obtained from the fit suggests that Actin-mediated modulations of cortical bending rigidity, as characterized by α_κ, play a crucial role in ACD and that changes in cortical Actin density have a much stronger effect on bending rigidity than on cortical tension (Extended Data Fig. 9i–k). This reveals a critical role of cortical Actin in determining daughter-cell sizes during ACD by providing substantial cortical bending elasticity.

Formin-mediated Actin is considered as a key determinant of cell mechanics⁶³ through its impact on cortical contractility⁶⁴. We show, however, that branched Actin modulates cortical mechanics to control size asymmetry in SOPs. This uncovers two fundamental aspects in cell mechanics: (1) branched cortical Actin can also play a key role for cell mechanics, and (2) not only contractility but also bending rigidity is important for cell morphogenesis.

Finally, we studied the mechanical stability of the two emerging spherical caps during furrow constriction. If the system is unstable, the furrow slips to one side: the smaller cap collapses into the bigger one. For the fit parameters obtained above, the system is least stable at the beginning of cytokinesis but stabilizes after 25% constriction (Extended Data Fig. 9i and Supplementary Information III.II.2). A reliable constriction during early cytokinesis can be achieved, for example, if constriction is faster than the collapse of the smaller cap or if branched Actin decreases cortical contractility⁶⁵ (Extended Data Fig. 9l,m and Supplementary Information III.II.4).

Cortical Actin determines size by modulating local curvature

Our theoretical model suggests that local changes in Actin density modulate the mechanical properties of the cell cortex locally to induce local shape changes of the cell contour. Indeed, when WAVE is mistargeted to the anterior cortex with nanobodies, local enrichment of branched Actin in patches correlates with a local cell contour flattening (Fig. 6d). To investigate this further, we measured the local enrichment of cortical Actin and the local cell curvature in normal conditions (Fig. 6e,f). At the cell poles, Actin density in the cortex correlates with curvature (Fig. 6f and Extended Data Fig. 9n). This suggests that Actin density itself could tune the cell contour curvature by modulating local cortical mechanics: flattening the cell surface contributes to the emergence of a larger daughter cell.

We then looked at the curvature dynamics while locally targeting WAVE in real time to the cortex though optogenetics (Fig. 6g–j and Supplementary Video 5). Figure 6i shows that cortical flattening upon local branched Actin enrichment happens in only a few minutes. These fast dynamics are compatible with a role of cortical Actin to shape cells during cytokinesis, which occurs at a timescale of about 15 min.

Discussion

Our work shows that polarized cortical Actin produces unequal-size daughters during ACD. In particular, branched Actin modulates the cytokinetic cell shape by locally setting the bending rigidity, and thereby determines the daughter-cell size asymmetry (for summary, see Extended Data Fig. 10a). This is based on the following key observations: (1) In wild type, cortical branched Actin accumulation is observed in the larger posterior pole (Fig. 1f,g) and local Actin density correlates with local curvature (Fig. 6d–f and Extended Data Fig. 9n). (2) When cortical Actin is symmetrical, the SOP divides with symmetric daughter sizes (Figs. 1 g, 2 b–d and 4e–g and Extended Data Figs. 2 c,d, 5 a–e and 6c–e). (3) When cortical Actin asymmetry is inverted, cell size is inverted (Figs. 3a–c and 5b–d). (4) When cortical branched Actin polymerization is induced locally by optogenetics, the cortex flattens locally (Fig. 6g–j).

During SOP division, an asymmetry in bending rigidity is sufficient to achieve asymmetric sizes (Extended Data Fig. 9i–k). Our theoretical approach shows that an Actin dependence of both bending rigidity and tension accounts quantitatively for the observed correlation between cortical Actin density and daughter-cell size in all experimental conditions (Fig. 6b,c). However, our data show that Actin impacts bending rigidity much more strongly than tension: cortical branched Actin hence sets the bending rigidity of the cell cortex. This uncovers a distinctive, fundamental role of bending rigidity, instead of cortical tension, for asymmetric daughter-cell size determination.

In systems such as the Drosophila and Caenorhabditis neuroblasts, cortical tension due to Myosin asymmetries has been proposed as a major player for generating asymmetric daughter-cell sizes^5,14,15. Here we propose that the modulation of daughter-cell sizes can be mediated by two mechanisms, each related to a specific population of Actin: linear Actin together with Myosin leading to tension asymmetries and branched and crosslinked Actin leading to bending rigidity asymmetries. However, the control of bending rigidity by branched Actin, which we discovered here, adds to the toolkit to generate stable ACDs with unequal daughter-cell sizes. Finally, our findings may have implications for the understanding of cortical mechanics during stem cell divisions, which are not only important for cell shape and tissue morphogenesis, but also for cell fate assignation and tumour growth.

Methods

Key resources table

Materials, reagents and resources used in this study can be found in Supplementary Table 1.

Experimental model and subject details

Fly handling, fly lines and maintenance

All fly stocks were maintained at 18 °C. Fly crosses were raised at 25 °C, then kept at 16 °C until pupariation, and shifted to different temperature depending on the experiments (25 °C or 29 °C as indicated in Supplementary Table 1). To express genes in the SOPs, the Neuralized promoter together with the UAS-Gal4 system was used. We also used the Gal80^ts protein to reduce the levels of expression. For example, we used Gal80^ts to achieve low levels of Zipper (Myosin Regulatory Heavy Chain) expression to prevent motor aggregates (Extended Data Fig. 4e) or to prevent embryonic lethality in Gβ13F^RNAi.

Transgenes used in this study

UAS-Cofilin::GFP (this study), UAS-GBP::mCherry::LOV (this study), UAS-GFP::Arpin (this study), UAS-CIB¹::Arpin (this study), UAS-PDZ::Pon (this study), UAS-CIB¹::WAVE (this study), UAS-GFP::Cdc42 (this study), UAS-GFP::WAVE (this study), UAS-Lifeact::CRY2^FL::mCherry (this study), UAS-Lifeact::mCherry (this study), UAS-FP670::Pon (this study), Ubi-mCherry::Pavarotti⁴⁸ (Gonzalez Lab), UAS-GBP::Pon⁴⁸ (Gonzalez Lab), UAS-GBP::mCherry::Pon⁴⁸ (Gonzalez Lab), UAS-GBP::Bazooka⁴⁸ (Gonzalez Lab), UAS-Sqh^E20E21::GFP⁶⁹ (gift from Thomas Lecuit), UAS-GFP::Utrophin^ABD (ref. ⁷⁰) (gift from Thomas Lecuit), UAS-WAVE::GFP⁷¹ (gift from Sven Bogdan), UAS-GFP::Fascin⁷² (gift from Francois Payre), UAS-Dia::GFP⁷³ (gift from Eduardo Moreno), UAS-Flare::GFP⁷⁴ (gift from Paul N. Adler), UAS-DsRed (gift from François Karch), UAS-Zipper::GFP⁷⁵ (gift from Andrea Brand), Zipper::GFP⁷⁶ (Flytrap CC01626), Cheerio::GFP⁷⁷ (Bloomington 60261), GFP::Rac1 (Bloomington 52285), Jupiter::GFP (Bloomington 6836), Tub-Gal80ts (Bloomington 7017), mud⁴ (ref. ⁴¹) (Bloomington 9563), Sqh::GFP (Bloomington 57144), Fimbrin::GFP (Bloomington 51562), α-Actinin::GFP (Bloomington 51573), UAS-nausicaa^RNAi (ref. ²⁴) (VDRC 31375), UAS-dsh^RNAi (ref. ⁷⁸) (VDRC 101525), UAS-Gβ13F^RNAi (ref. ⁷⁹) (VDRC 31257), UAS-mRFP::Pon⁸⁰, Neur-Gal4 (ref. ⁸¹), Ubi-GFP::Pavarotti⁸², UAS-GFP::Pon⁸³, UAS-lgl^3A (ref. ⁸⁴).

More details on the transgenes used can be found in Supplementary Table 1.

Detailed genotypes and temperatures

Detailed genotypes and temperatures can be found in Supplementary Table 1.

Neuroblast culture

Live imaging was performed as described previously⁸⁵. Briefly, brains were dissected in collagenase buffer and incubated in collagenase for 15 min (0.2 mg ml⁻¹ collagenase, Sigma C0130). Brains were manually dissociated into a culture medium (Schneider’s medium supplemented with glucose, fetal calf serum, fly extract and insulin) as in ref. ⁸⁵.

Fluorodish was first activated using a plasma cleaner (Harrick Plasma, PDC-32G) for 3 min and then coated with poly-l-lysine. Dissociated brains were transferred onto the activated and coated Fluorodish. Cells were allowed to settle and adhere to the coverslip 30 min before imaging.

Collagenase buffer composition

The composition of 10× collagenase buffer were as follows: 8 g NaCl (Panreac 131659.1211), 0.2 g KCl (Merck TA 52 0336), 0.05 g NaH₂PO₄ (Merck 13799), 1 g NaHCO₃, 1 g d(+)-glucose (Merck 20174 312) and quantum satis 100 ml H₂O and add 2 mg ml⁻¹ collagenase.

Schneider glucose supplemented composition

Schneider’s medium (Gibco 21720-024) supplemented with 1 mg ml⁻¹ glucose (Merck 20174 312).

Neuroblast culture medium composition

The composition of 2.5 ml of Schneider + glucose medium were as follows: 300 µl FBS (10%, Thermo Fisher #10270106), 75 µl fly extract (inactivated and filtered sterilized medium: 100 g of smashed Oregon flies into 680 ml of cold M3 insect medium, Millipore Sigma, #S3652), 3 µl insulin (Sigma 19278) and 30 µl P/S (Gibco 15140).

Plasmids

Most of the open reading frames cloned by PCR for this study were flanked by FseI and AscI (FA) sites for convenient shuttling between compatible plasmids.

UAS-GFP::WAVE: For GFP N-ter WAVE cloning, the Drosophila WAVE coding region was digested by FA from the plasmid: pCS2 GFP dWAVE, gift from Alexis Gautreau, and inserted into a Drosophila UAS expression plasmid pUAST4 PC GFP FA blue.
UAS-GFP::Arpin: Similarly, for GFP N-ter Arpin cloning, Arpin coding region was digested by FA from the plasmid pcDNA5 FRT His PC TEV Arpin, gift from Alexis Gautreau⁵⁰, and inserted into a Drosophila UAS expression plasmid pUAST4 PC GFP FA blue. N‐terminal GFP tagging of Arpin has been previously shown to be functional.
UAS-GBP::mCherry::LOV: We also cloned the GBP, or so-called GFP nanobody, a lama VHH single-chain antibody against GFP for expression of fusion proteins in the fly with Light Oxygen Voltage Sensing Domain (LOV), a photo-interacting protein able to interact with a PDZ domain from Avena sativa⁵⁸. The GBP nanobody has been previously cloned into a pUAST4 GBP FA blue plasmid as described in ref. ⁴⁸. mCherry-LOV, flanked with FA restriction sites, is a synthetic gene to remove unwanted AscI sites inside the sequence (IDT). We also added a flexible linker (glycine- and serine-rich linker) between mCherry and the LOV peptide. It was then inserted in C-ter into pUAST4 GBP FA blue plasmid.

For the synthetic mCherry::LOV gene and oligonucleotide sequences used, see Supplementary Table 1.
UAS-PDZ::Pon: ePDZb1, flanked with EcoR1 and FseI (EF) restriction sites, is a synthetic gene to remove unwanted EcoR1 sites inside the sequence (IDT). It was then inserted in N-ter into pUAST4 EF Pon^LD plasmid, where Pon^LD is the Pon Localization Domain (corresponding to amino acids 474–670 of the Pon protein⁸³) cloned from w¹¹¹⁸ flies cDNA into a pUAST4 Drosophila plasmid as described in ref. ⁴⁸.

For the synthetic PDZ gene and oligonucleotide sequences used, see Supplementary Table 1.
UAS-Lifeact::mCherry: Lifeact was amplified by PCR, flanked with FA, from pIRES puro Lifeact mCherry plasmid, gift from Guillaume Montagnac, and inserted into a pUAST4 FA mCherry plasmid.

For oligonucleotide sequences used, see Supplementary Table 1.
UAS-Lifeact::CRY2^FL::mCherry: For the full-length CRY2 protein cloning, mCherry-CRY2^FL was amplified by PCR, flanked with AscI and NotI (AN) digestion sites from the plasmid available from Addgene collection ID 26871 (pCRY2^FLdeltaNLS-mCherryN1) and was inserted in C-ter into pUAST4 FA Lifeact AN mCherry plasmid pre-digested by AN for replacement of mCherry by CRY2^FL-mCherry.

For oligonucleotide sequences used, see Supplementary Table 1.
UAS-CIB¹::GFP::Arpin: CIB¹-GFP was amplified by PCR, flanked with EF, from plasmids available from Addgene collection ID 28240 (pCIB¹deltaNLS-pmGFP) and was inserted in N-ter into pUAST4 EF GFP FA Arpin plasmid pre-digested by EF for replacement of GFP by CIB¹-GFP.

For oligonucleotide sequences used, see Supplementary Table 1.
UAS-CIB¹::GFP::WAVE: WAVE FA insert was isolated and purified from pUAST4 PC GFP FA WAVE and inserted into a pUAST4 CIB¹ GFP FA Arpin plasmid pre-digested by FA for replacement of Arpin by WAVE.
UAS-GFP::Cdc42: For the Cdc42 protein cloning, Cdc42 was amplified by PCR, flanked with FA digestion sites from the plasmid available from Addgene collection ID 52248 (pUASp YFP Cdc42 WT) and was inserted in C-ter into pUAST4 PC GFP FA plasmid pre-digested by FA for insertion of the Cdc42 FA fragment. The plasmid was then was sent to BestGene to be injected into w¹¹¹⁸ background Drosophila embryo to generate transgenics.

For oligonucleotide sequences used, see Supplementary Table 1.
UAS-FP670::Pon: Similarly to the UAS-PDZ::Pon cloning above, FP670 synthetic gene (IDT), flanked with EF restriction sites, was then inserted in phase and in N-ter into pUAST4 EF Pon^LD plasmid. The plasmid was then was sent to BestGene to be injected into w¹¹¹⁸ background Drosophila embryo to generate transgenics.

For the synthetic FP670 gene sequence, see Supplementary Table 1.
UAS-dCofilin::GFP: dCofilin corresponds to the Drosophila Twinstar protein. For dCofilin::GFP, a synthetic gene has been created with a GFP inserted in the middle, between N74 and G75, surrounding by 12 AA flexible linkers (GSA) on each side of GFP and with a STOP codon at the end. The synthetic gene has been flanked by FA sites. Post FA digestion, it was then inserted in N-ter into pUAST4 FA mCherry plasmid. For the synthetic Cofilin::GFP gene sequence, see Supplementary Table 1.

All the cloning has been confirmed by sequencing (Fasteris). All synthetic genes have been generated by IDT (G-blocks gene fragment). Injection of plasmids into Drosophila embryos to generate transgenics was performed by BestGene in w¹¹¹⁸ background.

Imaging method details

Fly notum imaging

Fly notum dissection and SOP imaging was performed in clone 8 medium after embedding into a fibrinogen clot in order to diminish tissue movements during fast 3D image acquisition as described⁸⁶. Imaging was performed using a 3i Marianas spinning disk confocal setup based on a Zeiss Z1 stand, a 63× PLAN APO NA 1.4 objective and a Yokogawa X1 spinning disk head followed by a 1.2× magnification lens and an Evolve EMCCD camera (Photometrics). Fast z-stack acquisition of entire SOP cells (0.4 µm steps) was obtained using a piezo stage (Mad City Labs). Single-emitter emission filters were always used to avoid bleed-through, and each channel was acquired sequentially. To increase acquisition speed, we acquired 3D stacks spanning only 16–18 µm along the z axis, which is usually sufficient to contain the entire dividing SOP. To assure linearity and avoid saturation, fluorescent signal was systematically checked to be within the camera dynamic range. Unless stated otherwise, data presented in figure panels correspond to maximum-intensity projections.

Clone 8 medium composition

The composition for 250 ml were as follows: 6.5 ml fly extract (inactivated and filtered sterilized medium: 100 g of smashed Oregon flies into 680 ml of cold M3 insect medium, Millipore Sigma, #S3652), 5 ml of foetal bovine serum (Thermo Fisher Scientific, #10270106), 125 µl insulin from bovine pancreas (Millipore Sigma, #I1882) and 238.62 ml Shields and Sang M3 Insect Medium (Millipore Sigma, #S3652).

SiR-DNA probe

To follow the different mitosis stages, after fibrinogen clot embedding described as above, clone 8 imaging media were supplemented with 1 μM SiR‐DNA⁸⁷ (Spirochrome) and incubated at least 10 min at room temperature before imaging and left into the imaging media until the end of the experiment.

FRAP

FRAP of Actin-GFP (Extended Data Fig. 1k,l) was performed on the fly genotype w¹¹¹⁸; UAS-GFP-Actin 5C/+; Neur-Gal4/+ on the 3i Marianas spinning disk confocal setup based on a Zeiss Z1 stand, a 63× PLAN APO NA 1.4 objective and a Yokogawa W1 spinning disk head followed by a 1.2× magnification lens and an iXon EMCCD Andor camera equipped with Vector Photomanipulation hardware, a high-speed galvanometer-based point scanner, driven by Slidebook 6.0.

Two circular bleaching regions (2 µm diameter) were drawn onto the anterior and the posterior cortex and bleached simultaneously on both poles in late anaphase. Owing to the fast recovery of Actin-GFP (timescale of few seconds), recovery was monitored in 2D (one z plane) to maximize frame rate and ensure acquisition is as fast as possible. The recovery was then monitored by spinning disk confocal imaging at an average frame rate of 4.3 Hz.

For each FRAP experiment, we followed four different regions:

(1)
Anterior FRAP region of interest (ROI);
(2)
Posterior FRAP ROI;
(3)
Photobleaching ROI: cytoplasmic ROI within the same cell to measure the decay in fluorescence due to the acquisition bleaching;
(4)
Background ROI: ROI outside the cell to measure the offset intensity.

The photobleaching and background curves have been fitted with single exponential functions of the form:

$$I\left( t \right) = A + Be^{ - \frac{{(t - t_0)}}{\tau }}$$

Anterior and posterior FRAP curves were first processed for signal background subtraction (with the ROI outside the cell) and for photobleaching correction (with the cytoplasmic ROI within the cell) as follows:

$$I_{{\mathrm{Anterior}}\,{\mathrm{FRAP}}\,{\mathrm{corrected}}}\left( t \right) = I_{{\mathrm{Anterior}}\,{\mathrm{FRAP}}}\left( t \right) - I_{{\mathrm{Background}}}(t)$$

$$I_{{\mathrm{Posterior}}\,{\mathrm{FRAP}}\,{\mathrm{corrected}}}\left( t \right) = I_{{\mathrm{Posterior}}\,{\mathrm{FRAP}}}\left( t \right) - I_{{\mathrm{Background}}}\left( t \right)$$

$$I_{{\mathrm{Photobleaching}}\,{\mathrm{corrected}}}\left( t \right) = I_{{\mathrm{Photobleaching}}}\left( t \right) - I_{{\mathrm{Background}}}(t)$$

FRAP curves were then processed for photobleaching correction (with the cytoplasmic ROI within the cell) through double normalization⁸⁸ as follows:

$$I_{{\mathrm{FRAP}}\,{\mathrm{double}}\,{\mathrm{norm}}}\left( t \right) = \frac{{I_{{\mathrm{Pre}} - {\mathrm{photobleaching}}\,{\mathrm{corrected}}}}}{{I_{{\mathrm{Pre}} - {\mathrm{FRAP}}\,{\mathrm{corrected}}}}}\frac{{I_{{\mathrm{FRAP}}\,{\mathrm{corrected}}}\left( t \right)}}{{I_{{\mathrm{Photobleaching}}\,{\mathrm{corrected}}}\left( t \right)}}$$

where I_{Pre-FRAP norm} and I_{Pre-photobleaching norm} correspond to the average pre-bleaching intensities in the FRAP ROIs and in the photobleaching ROI, respectively.

Additionally, we performed full-scale normalization according to the following formula:

$$I_{{\mathrm{FRAP}}\,{\mathrm{double}}\,{\mathrm{norm}}\,{\mathrm{full}}\,{\mathrm{scale}}}\left( t \right) = \frac{{I_{{\mathrm{FRAP}}\,{\mathrm{double}}\,{\mathrm{norm}}}\left( t \right) - I_{{\mathrm{FRAP}}\,{\mathrm{double}}\,{\mathrm{norm}}}\left( {{\mathrm{bleach}}} \right)}}{{I_{{\mathrm{Pre}} - {\mathrm{FRAP}}\,{\mathrm{double}}\,{\mathrm{norm}}} - I_{{\mathrm{FRAP}}\,{\mathrm{double}}\,{\mathrm{norm}}}\left( {{\mathrm{bleach}}} \right)}}$$

where I_{FRAP double norm} (bleach) is the intensity value just after bleaching and I_{Pre-FRAP double norm} the average intensity before bleaching of the double normalized data.

Both anterior and posterior normalized FRAP curves were then fitted to a double exponential equation (as previously established for Actin dynamics^89,90):

$$I_{{\mathrm{FRAP}}\,{\mathrm{double}}\,{\mathrm{norm}}\,{\mathrm{full}}\,{\mathrm{scale}}}\left( t \right) = A_1\left( {1 - e^{ - \frac{{(t - t_0)}}{{\tau _1}}}} \right) + A_2\left( {1 - e^{ - \frac{{(t - t_0)}}{{\tau _2}}}} \right)$$

In Extended Data Fig. 1l, the represented turnover is the average of τ₁ and τ₂. For the anterior pole τ₁ = 0.3 ± 0.08; τ₂ = 9.01 ± 0.9 and for the posterior pole τ₁ = 0.8 ± 0.32; τ₂ = 28.31 ± 2.2 and in Extended Data Fig. 1k, immobile fractions are: anterior IF =0.212 ± 0.06; posterior IF = −0.005 ± 0.05.

To fully describe the Actin turnovers at the cell cortex, the curves should additionally be corrected for a cytoplasmic diffusive recovery process as described in ref. ⁹¹. Yet, our FRAP experiment aims to compare the anterior and the posterior cortex dynamics, and we assume that this process is similar in both the anterior and the posterior cytoplasm.

Fly notum immunofluorescence

Fly notum staining was performed as previously described⁸⁶: In brief, fly nota were dissected in PEM (80 mM PIPES, 5 mM EGTA and 1 mM MgSO₄), then fixed during 20 min in PEM with 4% PFA (Electron Microscopy Science) followed by a 20 min incubation in PEM with 4% PFA and 0.2% Triton X-100. Nota were then processed for immunofluorescence using standard techniques.

For Actin filament visualization, we used Phalloidin 425 (from Sigma) at a 1/40 dilution. Coverslips were mounted in Prolong Gold anti-fade reagent (Molecular Probes). Image acquisition setting was then performed on the 3i Spinning disk confocal microscope W1 setup described above.

Drug treatments

To inhibit Formins, we incubated our tissue with 40 µM SMIFH2 drug.

To dissasemble spindles during division, we used the microtubule depolymerizing drug Colcemid at 10 µg ml⁻¹ together with Roscovitine (CDK inhibitor) at 25 µM to bypass the metaphase-arrest checkpoint.

Nanobody targeting

In this study, we used the ‘nanobody assay’ established in ref. ⁴⁸, consisting of a GBP (or so-called GFP nanobody), a lama VHH single chain antibody against GFP⁴⁷ fused with polarity proteins in the fly (GBP::Pon and GBP::Bazooka). GBP::Pon will segregate in the anterior cortex as the wild-type Pon protein, whereas GBP::Baz will segregate in the posterior cortex.

Flies co-expressing GBP::Pon and WAVE::GFP (Fig. 3a–c) displayed occasional spindle orientation defects reflected by a division plane orthogonal to the polarity. Cells showing such spindle orientation defects were excluded from subsequent analysis.

We also used Gal80^ts to achieve lower levels of GBP-Baz to prevent ectopic expression of Bazooka (Extended Data Fig. 5m,n).

Photo-interacting protein optogenetic experiments

Whole-cell illumination

Whole-cell photomanipulation in Fig. 5a,b and Extended Data Figs. 6a–d and 8j,k was performed on the 3i Marianas spinning disk X1 setup described above as a stack acquisition with 488 laser at 250–500 ms and 405 laser at 10–20 ms exposure time to promote the interaction between the LOV peptide and the PDZ sequence protein or the CRY2^FL and CIB¹ protein couple.

To target WAVE into the anterior pole upon light illumination, we used the w; UAS-Pon::PDZ, UAS-GBP::mCherry::LOV/+; Neur-Gal4/UAS-WAVE::GFP fly genotype (Fig. 5a,b and Extended Data Fig. 8j,k). We also performed the same experiment with C-ter GFP-tagged WAVE instead of N-ter and obtain similar results of Actin recruitment into the anterior cortex in w; UAS-Pon::PDZ, UAS-GBP::mCherry::LOV/+; Neur-Gal4/UAS-GFP::WAVE fly (data not shown).

To target Arpin into cortical Actin-rich regions for branched Actin inhibition upon light illumination, we used w; UAS-CIB¹::GFP::Arpin/+; UAS-Lifeact::mCherry::CRY2^FL, Neur-Gal4/+ fly genotype (Extended Data Fig. 6c,d).

Posterior illumination

Posterior illumination in Fig. 4c–g and Extended Data Fig. 6f–i was performed on a 3i Marianas spinning disk confocal W1 setup described above with 3i Phasor hardware, a spatial light modulator-based computer-generated holography, driven by Slidebook 6.0. We generated a squared ROI centred in the middle of the cell in z englobing the posterior compartment to promote the interaction between the CRY2^FL and CIB¹ protein couple within the posterior pole only. We used the Phasor 445 laser at 15–20% laser power for 500 ms exposure starting in anaphase onset and illuminated the region after each 3D stack acquisition. To target Arpin into the posterior cortex for branched Actin inhibition upon light illumination, we used the w; UAS-CIB¹::GFP::Arpin/+; UAS-Lifeact::mCherry::CRY2^FL, Neur-Gal4/+ fly genotype (Fig. 4e–g and Extended Data Fig. 6f–h) or w; UAS-CIB¹::GFP::Arpin/UAS-FP670::Pon; UAS-Lifeact::mCherry::CRY2^FL, Neur-Gal4/+ fly genotype (Extended Data Fig. 6i).

Local illumination

Local illumination in Fig. 6g–j was performed on a 3i Marianas spinning disk confocal W1 setup described above with the 3i Vector hardware. We used the vector 488 laser to illuminate a 3 µm circle ROI located in the anterior pole, the illumination ROI was centred in the middle of the cell in z for 50 ms to 100 ms exposure starting at the second timepoint and then performed after each 3D stack acquisition. We always located our ROI regions in the anterior pole for two reasons: (1) to avoid the influence of the posterior Actin enrichment observed in wild type and (2) because, in the smaller anterior pole, the curvature is higher and thus any contribution of branched Actin on the curvature of the cell cortex would have a more dramatic/visible effect in the anterior pole.

To target WAVE and promoting branched Actin polymerization into the small, illuminated ROI region at the cell cortex, we use the w; UAS-CIB¹::GFP::WAVE /+; UAS-Lifeact::mCherry::CRY2^FL, Neur-Gal4/+ fly genotype (Fig. 6g–j).

Laser ablation

Cortex ablation

Cortical laser ablation was performed on the 3i Marianas spinning disk setup described above (63× NA 1.4 oil objective) equipped with Vector coupled with Ablate! pulsed laser ablation hardware driven by Slidebook 6.0 (Extended Data Fig. 9c,d). To cut the cortex, laser ablation was performed on SOPs expressing w;; UAS-Lifeact::mCherry, Neur-Gal 4/+. We performed sequential cuts: first in the anterior cortex and then in the posterior cortex. As we observed bigger blebs (innate and laser-induced) in the posterior pole, we always performed ablation of the cortex at the anterior pole first in order to discard the effect of an intracellular pressure release at the first ablation, which could induce bigger blebs. In the quantification, we selected only cells that were not damaged by the ablation (where laser-induced blebs could retract after ablation and SOPs were able to finish cytokinesis). The bleb areas were measured when the bleb sizes reached their maximum size before retraction.

Neighbour cell ablation

Laser ablation was performed on the 3i Marianas spinning disk setup as described above in nota expressing w;; UAS-Lifeact::mCherry, Jupiter::GFP, Neur-Gal 4/+ (Extended Data Fig. 9e,f). We performed tissue ablation on entire pupae as described in ref. ⁹². To remove the impact of neighbour cells on the daughter-cell size asymmetry, we performed large cuts in the anterior side of dividing SOPs (in anaphase) to remove neighbour cells. We then measured daughter-cell size once cytokinesis was completed.

Scanning electron microscopy

Flies were killed by exposure to diethyl ether for 20 min, then mounted on scanning electron microscopy holders using double-sided carbon tape (Electron Microscopy Sciences) and subsequently treated with a gold sputter coater (JFC-1200, JEOL). Imaging was performed using a JEOL JSM-6510LV scanning electron microscope operating in high-vacuum mode using a working distance of 1 mm and an acceleration of 10–15 kV.

Statistics and reproducibility

Unless stated otherwise, measurements are given as mean ± standard error of the mean (s.e.m.). All statistical analyses were performed using SigmaStat 3.5 software (Systat). Normality of variables was verified with Shapiro–Wilk tests. Homoscedasticity of variables was always verified when conducting parametric tests. In cases where variables failed normality and/or homoscedasticity tests, non‐parametric tests were applied.

Micrographs are representative of a set of at least two independent experimental rounds performed on different days and were in all cases reproducible. No statistical method was used to pre-determine sample size. The experiments were not randomized. The Investigators were not blinded to allocation during experiments and outcome assessment. As disclosed above in Methods, SOP cells showing spindle orientation defects were excluded from the WAVE nanobody experiment and, for the dshRNAi experiment, only cells with polarity defects were selected (low phenotype penetrance). For more detail, see Reporting Summary.

Supplementary Note 1

For additional methods, in particular, on quantification and image analysis, please refer to section Supplementary Methods.

For additional information, please refer to section Supplementary Information.

For the theory, please refer to section Supplementary Theory.

Reporting summary

Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.

Data availability

Source data are provided with this paper. All other data supporting the findings of this study are available from the corresponding author on reasonable request.

Code availability

Custom codes written in ImageJ and MATLAB are available from the corresponding author upon reasonable request. Local curvature has been measured by an adapted MATLAB script from ref. ⁹³.

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Acknowledgements

This work has been supported by the Swiss National Science Foundation, the European Research Council, the NCCR Chemical Biology programme, the DIP of the Canton of Geneva and the SystemsX EpiPhysX (MGG). A.M. acknowledges support from an ELBE PhD fellowship, from an EMBO Longterm Fellowship (European Molecular Biology Organization, ALTF 528-2019) and from a DFG Postdoctoral Research Fellowship (Deutsche Forschungsgemeinschaft, Project 431144836), and F.J. was supported by the Cluster of Excellence Physics of Life, TU Dresden. In particular, we thank B. Baum, M. Fritzsche, Z. Hadjivasiliou and I. F. Sbalzarini for helpful discussions, K. Kruse, C. González, O. Afonso and M. Martinez Merino for critical reading of the manuscript, and J. Pampalona (from Cayetano González lab) and V. Sabado (from Emi Nagoshi lab) for training of the Drosophila neuroblast primary culture. We also thank M. Dubois for technical support and T. Lecuit, S. Bogdan, F. Payre, E. Moreno, P. N. Adler, F. Karch, A. Brand and M. Baylies for providing Drosophila fly lines, as well as Bloomington Drosophila Stock Center, Vienna Drosophila Resource Center and the Developmental Studies Hybridoma Bank for reagents.

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Authors and Affiliations

Department of Biochemistry, Faculty of Sciences, University of Geneva, Geneva, Switzerland
Alicia Daeden, Emmanuel Derivery, Carole Seum & Marcos Gonzalez-Gaitan
Department of Mathematics, Massachusetts Institute of Technology, Cambridge, MA, USA
Alexander Mietke
Max Planck Institute for the Physics of Complex Systems, Dresden, Germany
Alexander Mietke & Frank Jülicher
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Alexander Mietke
Center for Systems Biology Dresden, Dresden, Germany
Alexander Mietke
MRC Laboratory of Molecular Biology, Cambridge, UK
Emmanuel Derivery

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Alicia Daeden
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Alexander Mietke
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Emmanuel Derivery
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Carole Seum
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Frank Jülicher
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Marcos Gonzalez-Gaitan
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Contributions

A.D. conducted most of the genetic experiments, imaging and image analysis. A.M. and F.J. developed the theory and A.M. contributed to the 3D image analysis. E.D. and C.S. performed additional genetic experiments. A.D. and M.G.-G. designed the project.

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Correspondence to Marcos Gonzalez-Gaitan.

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Nature Cell Biology thanks Fumio Matsuzaki, Jens Januschke, Roland Wedlich-Söldner and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

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Extended data

Extended Data Fig. 1 Daughter-cell size and cortical Actin asymmetry.

a) PIIA versus PIIB daughter-cell volumes (Correlation factor: V_PIIA = 0.55V_PIIB). b) Projected Area (PA) measurements for each daughter-cell (PA_PIIA / PA_PIIB = 1.4 ± 0.2; n = 24, T-Test two-tailed, P < 0.001). c) Correlation between volume and projected area of the two daughter-cells. Projected area measurement is a reliable proxy for daughter-cell volumes. Power law correlation (dashed line; R² = 0.98). d) Phalloidin staining in SOPs expressing mRFP-Pon revealing filamentous Actin (F-Actin). e) Kymographs of the Lifeact signal along cytokinesis. For representation purpose, intensity has been color-coded using the Rainbow lookup table (red: left and LUT: right). White arrowheads: posterior cortical Actin enrichment; Yellow arrowheads: blebbing phenomena occurring in late cytokinesis stages mostly in the posterior compartment (See Extended Data Fig. 9a, b). f) Dynamics of the cortical Actin posterior enrichment in SOPs expressing Lifeact-mCherry (n = 4; Average + /− StDev). Posterior Actin Enrichment = $\frac{{{{{\mathrm{Posterior}}}}_{{{{\mathrm{Cortical}}}}\,{{{\mathrm{Actin}}}}}-{{{\mathrm{Anterior}}}}_{{{{\mathrm{Cortical}}}}\,{{{\mathrm{Actin}}}}}}}{{{{{\mathrm{Anterior}}}}_{{{{\mathrm{Cortical}}}}\,{{{\mathrm{Actin}}}}}}} \times 100$ (see Supplementary Methods). g) Averaged signal intensities from linescans across SOPs in late anaphase expressing Lifeact mCherry (n = 8). Violet arrowhead = anterior cortical Actin; Orange arrowhead = posterior cortical Actin. h) Upper panel: SOP expressing UAS-Utrophin-GFP (a marker for F-Actin) driven by the Neuralized promoter. Lower panel: Signal intensity from linescan of the Utrophin signal. i) FWHM measurements from linescans over the anterior cortex (left) or posterior cortex (right) from SOP expressing Lifeact mCherry (see Supplementary Methods). j) FWHM values as a proxy of the cortical thickness between the anterior and the posterior cortex. Mann-Whitney Rank Sum Test two-sided, P < 0.001. k) Fluorescence recovery monitored over time after photobleaching (FRAP) of GFP-Actin at the cell cortex (Left: FRAP at anterior cortex; Right: FRAP at the posterior cortex; Mean + /- SEM). Curves were fitted to a double exponential model (see Methods, violet and oranges lines, respectively). l) Average turnovers of GFP-Actin from FRAP experiments on the anterior and posterior cortex (average of the two τ from the double exponential fit: ($\frac{{\tau 1 + \tau 2}}{2}$), for the anterior pole τ₁ = 0.3 ± 0.08; τ₂ = 9.01 ± 0.9 and for the posterior pole τ₁ = 0.8 ± 0.32; τ₂ = 28.31 ± 2.2). Mann-Whitney Rank Sum Test two-sided, P < 0.001. All data are presented as Mean ± SEM unless stated otherwise. For details on genotypes, in this and other figures in this report, see Supplementary Table 1. n = number of cells from 6, 6, 6, 2, 4, 4, 9, 10 pupae for a, b, c, f, g, h, j, l respectively. Source numerical data are available in source data.

Source data

Extended Data Fig. 2 Cortical Actin asymmetry: Neuroblasts and importance of branched Actin.

a) Time-lapse spinning disk confocal imaging of representative dividing Neuroblast (from Drosophila brain primary culture) expressing Jupiter-GFP and Lifeact-mCherry (in red, upper panel and LUT, lower panel). Blue asterisks: GMC (ganglion mother cell) daughter-cells from precedent divisions. The neuroblast division (dashed white line) gives rise to a newly formed GMC (violet asterisk, smaller daughter-cell; basal side in whole brain) and a newly formed neural stem cell (orange asterisk, apical pole, bigger daughter-cell). Note the enrichment of Actin in the apical pole (bigger pole, future neuroblast, orange asterisk) in the third timepoint. White arrowheads and dashed blue lines highlight blebs in the apical pole in the third and fourth timepoints (middle panel). b) Cortical Actin asymmetry in the posterior cortex in SOPs versus Neuroblasts. Actin asymmetry=($\frac{{{{{\mathrm{I}}}}_{{{{\mathrm{Posterior}}}}\,{{{\mathrm{Cortical}}}}\,{{{\mathrm{Actin}}}}} - {{{\mathrm{I}}}}_{{{{\mathrm{Anterior}}}}\,{{{\mathrm{Cortical}}}}\,{{{\mathrm{Actin}}}}}}}{{{{{\mathrm{I}}}}_{{{{\mathrm{Anterior}}}}\,{{{\mathrm{Cortical}}}}\,{{{\mathrm{Actin}}}}} + {{{\mathrm{I}}}}_{{{{\mathrm{Posterior}}}}\,{{{\mathrm{Cortical}}}}\,{{{\mathrm{Actin}}}}}}}$) measured by the ‘Linescan’ method - See Supplementary Methods). T-Test one-tailed (P < 0.05). c,d) Maximum projection (c) and corresponding linescans of the Lifeact-mCherry signal (d) of SOPs expressing nausicaa^RNAi (naus^RNAi; pink) together with Lifeact-mCherry under the Neuralized promoter. Arrowheads, cortical Actin. Note the loss of the cortical Actin asymmetry in naus^RNAi conditions. e,f) Time-lapse spinning disk confocal imaging of a SOP expressing Lifeact-mCherry under the Neuralized promoter and incubated with the SMIFH2 Formin inhibitor drug (e) and corresponding linescan of the Lifeact-GFP signal (f). Note that upon addition of the SMIFH2 drug, both daughter size and Actin asymmetries are still visible. All data are presented as mean values +/− SEM. n = number of cells from 4 pupae for SOPs and 6 third instar larvae for Neuroblasts in b and 4, 3 pupae in d, f respectively. Scale bar 5μm. Source numerical data are available in source data.

Source data

Extended Data Fig. 3 A branched and crosslinked posterior cortical Actin.

a-j) Time-lapse spinning disk confocal imaging of a SOP expressing endogenous GFP-Rac1 (Ras-related GTPases regulating branched F-Actin growth) (a), UAS GFP-Cdc42 (Ras-related GTPases regulating branched F-Actin growth) (c), Dia-GFP (Diaphanous-related Formin, Formins Actin nucleators regulating linear long F-Actin (e), UAS Fascin-GFP (g) and Cheerio-GFP (Drosophila ortholog of mammalian Filamin, Actin Crosslinker; one z-plane) (i) driven by the Neuralized promoter. Averaged signal intensities in b, d, f, h, j from linescans across SOPs in late anaphase to visualize the fluorescence signal observed in a, c, e, g, i. Note the accumulation of Rac (b), Cdc42 (d) and Filamin (j) in the posterior cortex (arrowheads) and the absence of cortical accumulation of Formins (f) and Fascin (h) at the cell poles. Note that Rac1 and Cdc42 tagged proteins might reflect both an active and an inactive pool. It is also worth noting that, in other organisms, Rac has been reported to bind to some Formins (such as FHOD in Mouse fibroblasts⁹⁴), although the functional relevance of this interaction remains unclear^95,96. Also, Cdc42 has been reported to be upstream Formins during planar cell polarity in the Drosophila eye⁹⁷. Scale bars 5μm. All data are presented as Mean ± SEM. n = number of cells from 4, 4, 3, 5, 3 pupae for b, d, f, h, j respectively. Source numerical data are available in source data.

Source data

Extended Data Fig. 4 Polar targeting of Myosin and Actin bundling crosslinkers decreases daughter-cell size.

a, b) Time-lapse spinning disk confocal images showing expression of Lifeact-mCherry (a, b; red) under the under the Neutralized promoter in SOPs together with expression of endogenous α-Actinin-GFP (a; green) or endogenous Fimbrin-GFP (b; green) in the whole epithelium. Note the absence of posterior cortical enrichment or asymmetry for either α-Actinin-GFP and Fimbrin-GFP (yellow arrowheads, third timepoint). c,d) Time-lapse spinning disk confocal images showing expression under their own promoter in the whole epithelium of Myosin Regulatory Light Chain (MRLC; green; c) or Myosin Heavy Chain (MHC; red; d) fused to GFP. One z plane. e) Overexpression of MHC-GFP under the Neutralized promoter in SOPs. Maximal projection. Upon MHC overexpression, aggregates of myosin motors are observed. We could not detect any asymmetry in the cortical Myosin between the two cell poles (third timepoints in (c-e); yellow arrowheads). Rainbow LUT (lower panel of c-e). f,g) Time-lapse spinning disk confocal images showing expression of Lifeact-mCherry (red) together with the nanobody GBP-Pon (untagged) under the under the Neutralized promoter in SOPs as well as expression of endogenous α-Actinin-GFP (f; green) or endogenous Fimbrin-GFP (g; green) in the whole epithelium. h-j) Time-lapse spinning disk confocal images showing expression of RFP-Pon (h, j; red), Lifeact-mCherry (i; red), GBP-Pon, (h, i) or GBP-Baz (j) under the under the Neutralized promoter in SOPs as well as expression under their own promoter, in the whole epithelium of MRLC^WT-GFP (h, j; green) or constitutively active MRLC^CA (i; green), k) Metaphase SOP expressing MRLC-GFP under its own promoter in the whole epithelium (green) as well as UAS-RFP-Pon and UAS-GBP-Pon under the Neutralized promoter in SOPs. We observed a higher curvature in the anterior pole where Myosin is targeted (k), leading ultimately to a smaller anterior daughter-cell (h, i, l). l) Daughter-cell projected area ratios showing an enhancement of the daughter-cell size asymmetry when Myosin is targeted to the anterior cortex or a more symmetrical daughter sizes when Myosin is targeted to the posterior cortex (Kruskal-Wallis non-parametric one-way ANOVA followed by Holm-Sidak test, P < 0.001). Data are presented as Mean ± SEM. n = number of cells in l from 10, 5, 3, 5, 6, 9 pupae from left to right respectively. Scale Bar 5μm. Source numerical data are available in source data.

Source data

Extended Data Fig. 5 Other polarity cues, optogenetics and nanobody mistargeting of WAVE.

a-d) Maximum projection (a, c) and corresponding linescans of the Lifeact-mCherry signal (b, d) of SOPs expressing GFP-Pon and dishevelled^RNAi (a: dsh^RNAi; blue) or the heterotrimeric protein Gβ13F^RNAi (c: Gβ13F^RNAi; yellow) together with Lifeact-mCherry under the Neuralized promoter. Arrowheads, cortical Actin. Note that for dsh^RNAi (a, b, e) only SOPs with polarity defects (reflected by the Pon signal) were selected (less than 10% penetrant phenotype) and for Gβ13F^RNA (d), only symmetrical cells were selected for the averaged linescans (n = 13). e) Projected area ratio of daughter-cells ($\frac{{{{{\mathrm{Area}}}}_{{{{\mathrm{Posterior}}}}\,{{{\mathrm{PIIA}}}}}}}{{{{{\mathrm{Area}}}}_{{{{\mathrm{Anterior}}}}\,{{{\mathrm{PIIB}}}}}}}$); Kruskal-Wallis non-parametric one-way ANOVA followed by Dunn’s post hoc test (P < 0.001). Note that the Control and the naus^RNAi data are shown again from Fig. 2b. f) Raw (unbinned) data, showed in Fig. 2g, of Posterior cortical Actin enrichment $\Delta {{{\mathrm{I}}}} = \frac{{(I_2 - I_1)}}{{(I_1 + I_2)}}$ versus Daughter-cell size ratio ($\frac{{{{{\mathrm{Area}}}}_{{{{\mathrm{Posterior}}}}}}}{{{{{\mathrm{Area}}}}_{{{{\mathrm{Anterior}}}}}}}$) in mud⁴ mutant. Dashed line, linear Fit. g, h) Time-lapse spinning disk confocal imaging of a SOP expressing WAVE-GFP (green) under the Neuralized promoter (g) and its associates linescan (h) across the dashed white arrows (20-pixel width linescan centered around the white dashed lines) shown in g. i) Phalloïdin staining (yellow; far-red probe) of SOPs expressing WAVE-GFP (green), Lifeact-mCherry (red) and the nanobody GBP-Pon under the Neuralized promoter. The anterior targeting of WAVE generates an anterior Lifeact crescent (both F-Actin and G-Actin specific) colocalizing with the Phalloïdin staining (only F-Actin). j) Signal intensity along linescans from SOPs expressing WAVE-GFP, Lifeact-mCherry (red) and the nanobody GBP-Pon under the Neuralized promoter to visualize Lifeact (red; left panel) and WAVE (green; right panel). k) Maximum projection of SOPs expressing Lifeact-CRY2^FL-mCherry (red) and CIB¹-GFP-WAVE and UAS-lgl^3A under the Neuralized promoter. In the control (upper panel), CRY2^FL/CIB¹ interaction has not been activated by blue light and therefore CIB¹-GFP-WAVE is not targeted to one pole by optogenetics. Optogenetic targeting (lower panel) of CIB¹-GFP-WAVE is achieved by polar illumination with blue light (dashed box) at anaphase onset. Timing of blue light illumination is indicated. Note that while in lgl^3A conditions, SOPs divide with symmetric size, targeting of WAVE to the one pole leads to asymmetric daughter size. l) Daughter-cell projected areas (posterior/anterior) in conditions shown in k. T-Test two-tailed (P < 0.001). m) Maximum projection of SOPs expressing WAVE-GFP, Lifeact-mCherry and the nanobody GBP-Baz under the Neuralized promoter. n) Signal intensities along linescans from SOPs expressing WAVE-GFP (green), Lifeact-mCherry (red) and GBP-Baz under the Neuralized promoter to visualize Lifeact (red; lower panel) and WAVE (green; upper panel). All data are presented as mean values +/− SEM unless stated otherwise. n = number of cells from 3, 4, 16, 7, 5, 2 pupae for b, d, f, h, j, n respectively, from 10, 3, 7, 8, 3 pupae for e and 11, 5 pupae for l from left to right respectively. Scale bars: 5 µm. Source numerical data are available in source data.

Source data

Extended Data Fig. 6 Impairing Actin asymmetry through optogenetics leads to symmetric daughters.

a) Maximum projection of a SOP expressing Lifeact-CRY2^FL-mCherry (Red) under the Neuralized promoter. b) Signal intensity of the Lifeact-mCherry channel from linescans along SOPs as in a (third timepoint). c) Maximum projection of a SOP expressing Lifeact-CRY2^FL-mCherry (Red) and CIB¹-GFP-Arpin under the Neuralized promoter. Upon blue light illumination of the whole cell in anaphase A (second timepoint), CRY2^FL and CIB¹ interact leading to the recruitment of Arpin into Actin rich regions. d) Signal intensity of the Lifeact-mCherry channel from linescans along the SOP showed in c (upper panel) or averaged linescans (n=12; lower panel). Note the loss of the cortical Actin enrichment at the posterior pole (d), ultimately leading to symmetric daughter-cell size (c). e) Daughter-cell size ratio (projected areas ratio, Kruskal-Wallis non-parametric one-way ANOVA followed by Dunn’s post hoc test, P < 0.001). Note that ‘No Light’ and ‘Posterior Illumination’ data are shown again from Fig. 4g. f) Maximum projection of an SOP expressing Lifeact-CRY2^FL-mCherry (Red) and CIB¹-GFP-Arpin under the Neuralized promoter together with SiR-DNA probe (green). g) DNA metaphase plate (probed by SiR-DNA) positions as shown in f. The middle of the cell has been positioned to 0.5 highlighted by the dashed line. Mann-Whitney Rank Sum Test two-sided, N.S. h) Averaged signal intensities from linescans across SOPs in metaphase incubated with SiR-DNA, as shown in f. i) Maximum projection of a SOP expressing Lifeact-CRY2^FL-mCherry (Red), CIB¹-GFP-Arpin and FP670-Pon (green) under the Neuralized promoter. Note that the asymmetric localization of Pon (cell polarity marker) in the anterior pole is not affected upon optogenetic targeting of Arpin in the posterior pole. All data are presented as Mean ± SEM. n = number of cells from 3, 5, 4 pupae for b, d, h respectively and from 10, 6, 10, 3, 5 pupae for e from left to right and 4, 4 pupae for g from top to bottom respectively. Scale Bar 5 µm. Source numerical data are available in source data.

Source data

Extended Data Fig. 7 Nanobody mistargeting of Actin Depolymerizing Factors (ADF).

a) Maximum projection of SOP expressing GFP-Arpin under the Neuralized promoter. Note the cytosolic localization of the Arpin signal. b-g) Maximum projection of SOP expressing Lifeact-mCherry (green), GBP-Pon and GFP-Arpin (red) (b), AIP1-GFP (Flare-GFP in Drosophila, an Actin depolymerizing factor) (d) or Cofilin-GFP (Drosophila Cofilin, an Actin filament severing factor) (f) under the Neuralized promoter. Corresponding signal intensities showed in c, e, g from linescans across SOPs in late anaphase to visualize the fluorescence signal observed in b, d, f. Scale bars 5μm. h) Daughter-cell size ratio (projected areas ratio). The co-expression of the nanobody GBP-Pon together with Actin Depolymerizing Factors leads to an enhancement of the daughter-cell size asymmetry (One-way ANOVA test followed by a Holm-Sidak post hoc test, P < 0.001). All data are presented as mean values +/− SEM. Note that ‘Control’ data are shown again from Fig. 3c. n = number of cells from 8, 7, 4 pupae for c, e, g respectively and 8, 5, 8, 7, 4 pupae for h from left to right respectively. Source numerical data are available in source data.

Source data

Extended Data Fig. 8 Asymmetric positioning of the mitotic spindle does not govern daughter-cell sizes.

a) MAX-Projection of a metaphase SOPs expressing Jupiter-GFP (microtubules; red) and Lifeact-mCherry (blue) together with the far-red probe SiR-DNA (green). b, c) Averaged signal intensities from linescans across SOPs in metaphase tagged with SiR-DNA (b) or expressing Jupiter-GFP (c). The middle of the cell has been positioned to 0.5 highlighted by the dashed line. d) Hemi-spindle length measurements in metaphase SOPs (Mann-Whitney Rank Sum Test two-sided, P < 0.01). e) Maximum projections of time-lapse spinning disk confocal imaging of SOP expressing Lifeact-mCherry (green), Pavarotti-GFP (red) together with the far-red probe SiR-DNA (magenta). f) DNA metaphase plate and central spindle complex (probed by Pavarotti-GFP) positions. From geometry constrains only, this mild asymmetry of spindle positioning (b-d) leading to the mild asymmetry of the actomyosin ring positioning (e-f) is not sufficient to explain the final asymmetry of daughter sizes observed during SOP division (See Supplementary Information II.II). g, h) Maximum projection of SOP expressing Lifeact-mCherry (Red) under the Neuralized promoter together with endogenous Jupiter-GFP (microtubule; green) incubated without (g) or with (h) the microtubule depolymerizing drug Colcemid (+ Roscovitine to bypass the metaphase-arrest checkpoint). Note the disappearance of the microtubule signal in the Colcemid conditions. i) Pole size area ratios between control and cells treated with Colcemid + Roscovitine to depolymerize all microtubules (T-Test two-tailed, N.S.). All data are presented as mean values +/− SEM. n = number of cells from 3 pupae for b, c, d respectively, 4, 2 pupae for f from top to bottom and 4, 4 pupae for i from left to right respectively. Scale Bar 5μm. Source numerical data are available in source data.

Source data

Extended Data Fig. 9 Asymmetric cortical mechanics, parameterization of surface shapes through spherical caps and mechanical shape stability.

a) Time-lapse spinning disk confocal imaging of SOPs at late stages of cytokinesis expressing Lifeact mCherry (upper panel) or endogenous Spaghetti Squash (lower panel). We could observe accumulation and bursts of Myosin at the end of the cytokinesis (mainly in the posterior pole) due to blebbing: Myosin has been shown to be required in the bleb retraction process^98,99,100. b) Number of blebs per minute observed at the end of the division in the anterior and in the posterior poles of the dividing cells (Mean +/− SEM, Mann-Whitney Rank Sum Test two-sided, P < 0.001). c) Laser-induced bleb from laser ablation of the cell cortex. Snapshots of timepoints before (red) and after laser ablation (green). d) Laser-induced bleb area measurements from anterior cortex ablation and posterior cortex ablation (Mean +/− SEM, Mann-Whitney Rank Sum Test two-sided, P < 0.001). Blebs have been shown to be reporters of local cortical tension and their size to be proportionally correlated with cortical tension⁵⁹. Therefore, this asymmetric blebbing (innate and induced) uncovers a higher effective tension in the posterior cortex. e) Asymmetric removal of the neighbour cells does not impact daughter-cell size during SOP division. Scale bar 5 µm. f) Daughter-cell size ratio between control (Intact Tissue) and tissue where all the neighbour cells anterior to the SOP have been removed by laser ablation (Neighbours Removal). This corroborates the idea that daughter size is controlled only by an intrinsic mechanism relying on the cell cortex. Mean +/− SEM, T-Test two-tailed, N.S. g) We consider surface shapes with fixed volume V₀ and corresponding reference radius R₀. Cell divisions are represented by two connected spherical caps that are characterized by corresponding radii R_i, surface areas A_i, opening angles θ_i and the furrow radius r_f. The projected areas a₁ and a₂ (light shaded orange and violet regions) are used to compare model geometries to experimentally measured projected area asymmetries $\Delta a = \frac{{(a_2 - a_1)}}{{(a_1 + a_2)}}$. h) Daughter-cell size asymmetry Δa versus cortical Actin asymmetry $\Delta I = \frac{{(I_2 - I_1)}}{{(I_1 + I_2)}}$ (binned shown in Fig. 6b, c). i) Linear stability diagram of symmetric surfaces (A₁=A₂) with homogeneous material properties σ₁=σ₂₌σ, κ₁=κ₂=κ and at different relative furrow radii $\overline {r_f} = r_f/R_0$. A symmetric position of the neck is stable for all furrow radii in the blue-shaded region. Red dot indicates best fit of the minimal model (see i, k). j) Shape asymmetries $\Delta a = \frac{{(a_2 - a_1)}}{{(a_1 + a_2)}}$ resulting from bending rigidity asymmetries $\Delta \kappa = \frac{{(\kappa _2 - \kappa _1)}}{2}$ and surface tension asymmetries $\Delta \sigma = \frac{{(\sigma _2 - \sigma _1)}}{2}$. Black dashed line depicts symmetric surface geometries ($\Delta a = 0$). Considering a fixed mean tension σ_m, the mean bending rigidity was set to $\frac{{\kappa _m}}{{\sigma _mR_0^2}} = 7.1$. k) Least squares fit error of shape trajectories for different fit parameters $\frac{{\kappa _i}}{{\sigma _iR_0^2}} = \frac{{\alpha _\kappa }}{{\alpha _\sigma R_0^2}}$ (see Eqs. (12) and (13) from Supplementary Info. Theory) and R₀C₀ with the best fit shown in Fig. 6b, c. Intensity asymmetries $\Delta I = \frac{{(I_2 - I_1)}}{{(I_1 + I_2)}}$ translate to bending rigidities and surface tensions as described by Eqs. (12) and (13) (See Supplementary Info. Theory Section). The shape trajectory depicted in Fig. 6b, c (red line of the model) can therefore be represented in j as well (red solid line). In the white regions of j and k no (stable) equilibrium shapes exist or they represent improper constricted surfaces ($\theta _i > \pi /2$, see g). l, m) Theoretical shape curves for alternative scenarios that provide mechanical shape stability at early stages. I) Scenario in which increasing branched Actin density increases bending rigidity but decreases tension (l) (See Supplementary Info. Theory Section III.II.4). II) Scenario in which increasing Actin density increases tension but decreases local bending rigidity (m) (See Supplementary Info. Theory Section III.II.4). In both cases l and m, different pairs of Actin density (I₁,I₂), that yield the same values of normalized density difference $\Delta I = \frac{{(I_2 - I_1)}}{{(I_1 + I_2)}}$, can give rise to different projected area asymmetries $\Delta a = \frac{{(a_2 - a_1)}}{{(a_1 + a_2)}}$. Solid line and error bars depict the corresponding mean and standard deviation, respectively. n) Cortical Actin density versus local curvature along the cell contour. At the furrow region (curvature < 0), cortex highly enriched with Actin and Myosin at the actomyosin ring corresponds to negative curvature. At the cell poles (curvature ≥ 0), cortex with low cortical Actin show highly curved contours whereas cortex highly enriched with Actin corresponds to flatter contours (curvature = 0). Mean +/− StDev. Insert: Raw data. Source numerical data are available in source data. n = number of cells from 6, 6 pupae for b, d respectively, 6, 9 pupae for f from left to right respectively, 38 pupae for h, l and m, and 8 pupae for n.

Source data

Extended Data Fig. 10 Summary of observations to manipulate daughter-cell sizes during asymmetric cell division.

Summary of main observations. An asymmetric cortical Actin is observed in wild-type with more branched Actin in the posterior pole. When Actin asymmetry is inversed (that is, through WAVE nanobody targeting to promote branched Actin polymerization in the anterior cortex), cell size inversion is observed. Conversely, when Actin asymmetry is impaired in the posterior pole (that is, through lgl^3A overexpression or Actin-Depolymerization Factors (ADF) nanobody targeting to inhibit Actin polymerization), symmetric daughter-cells is observed. When Actin asymmetry is enhanced (that is, through ADF nanobody targeting to inhibit Actin polymerization in the anterior pole), an enhancement of the asymmetric size between the two daughter-cells is observed. Local branched Actin enrichment at the cell cortex, through optogenetic WAVE recruitment, was leading to lower curvatures of the cell contour.

Supplementary information

Supplementary Information

Supplementary methods, information, theory and references.

Reporting Summary

Supplementary Table 1

Key resources table. Detailed genotypes and temperatures. Oligonucleotides. Synthetic gene constructs.

Supplementary Video 1

Actin asymmetry during SOP division. SOP expressing Lifeact-mCherry (red) under the Neuralized promoter. Posterior enrichment of cortical Actin can be observed in late anaphase. Right: look-up table (LUT); redder pixels correspond to high fluorescence signals. Scale bar, 5 µm.

Supplementary Video 2

Inversion of Actin asymmetry leads to inversion of daughter-cell size. SOP expressing WAVE-GFP (green), Lifeact-mCherry (red) and GBP-Pon, an anti-GFP nanobody fused to the localization domain of Pon under the Neuralized promoter. Upon nanobody targeting of WAVE-GFP to the anterior pole, an enrichment of Actin (red) is visible, leading at the end of the division to an inversed asymmetric cell size. Scale bar, 5 µm.

Supplementary Video 3

Impairing cortical branched Actin at the posterior pole through optogenetics leads to symmetric daughters. SOP expressing Lifeact-CRY2^FL-mCherry (red) and CIB¹-GFP-Arpin under the Neuralized promoter. Optogenetic targeting of CIB¹-GFP-Arpin to the posterior cortex by posterior illumination with blue light (blue box) at anaphase onset leads to the loss of the posterior Actin enrichment and ultimately to symmetrical daughter cell. Scale bars, 5 µm.

Supplementary Video 4

Late-anaphase manipulation of cortical Actin leads to inversion of daughter-cell size. SOP expressing the opto-nanobody GBP-mCherry-LOV (red), WAVE-GFP (green) and PDZ-Pon (unlabelled) under the Neuralized promoter together with the SiR-DNA far-red probe (white) to define mitotic phases. Upon blue light illumination, in late anaphase, LOV and PDZ interact leading to WAVE targeting into the anterior pole. Anterior enrichment of branched Actin through optogenetics, up to late anaphase, leads to daughter-cell size inversion. Scale bars, 5 µm.

Supplementary Video 5

Local branched Actin optogenetics to manipulate cell curvature. SOP expressing Lifeact-CRY2^FL-mCherry (white) and CIB¹-GFP-WAVE under the Neuralized promoter. Upon blue light illumination in the small circle ROI (blue circle), CRY2^FL and CIB¹ interact leading to WAVE targeting in the illuminated cortical region. Branched Actin accumulates at the cortex and the cell contour becomes flatter in the ROI region. Scale bars, 5 µm.

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Daeden, A., Mietke, A., Derivery, E. et al. Polarized branched Actin modulates cortical mechanics to produce unequal-size daughters during asymmetric division. Nat Cell Biol 25, 235–245 (2023). https://doi.org/10.1038/s41556-022-01058-9

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Received: 03 August 2021
Accepted: 22 November 2022
Published: 06 February 2023
Issue Date: February 2023
DOI: https://doi.org/10.1038/s41556-022-01058-9

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Abstract

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Main

Results

Daughter-cell size asymmetry during SOP division

Cortical Actin asymmetry during asymmetric mitosis

Actin asymmetry coupled with PAR complex and daughter size

Polar Actin asymmetry levels correlate with daughter size

Nanobody mistargeting of Actin can invert size asymmetry

Impairing cortical branched Actin at the cell poles

Polar Actin acts beyond spindle positioning

Physics of daughter-cell size asymmetry

Cortical Actin determines size by modulating local curvature

Discussion

Methods

Key resources table

Experimental model and subject details

Fly handling, fly lines and maintenance

Transgenes used in this study

Detailed genotypes and temperatures

Neuroblast culture

Collagenase buffer composition

Schneider glucose supplemented composition

Neuroblast culture medium composition

Plasmids

Imaging method details

Fly notum imaging

Clone 8 medium composition

SiR-DNA probe

FRAP

Fly notum immunofluorescence

Drug treatments

Nanobody targeting

Photo-interacting protein optogenetic experiments

Whole-cell illumination

Posterior illumination

Local illumination

Laser ablation

Cortex ablation

Neighbour cell ablation

Scanning electron microscopy

Statistics and reproducibility

Supplementary Note 1

Reporting summary

Data availability

Code availability

References

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