Different methods were used to disrupt cell wall for extraction of camellia bee pollen protein isolates (CBPPI).
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The yield of CBPPI was affected by wall disruption methods.
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The disruption methods also affected the functional properties of CBPPI.
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Enzymatic hydrolysis improved the yield and functional properties of CBPPI.
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Combination with ultrasound further improved the yield and functional properties.
Abstract
Camellia bee pollen protein isolates were extracted by cell wall disruption using ultrasonication, freeze-thawing, enzymatic hydrolysis, and their combinations. The effects of these methods on microstructure of cell wall, protein release, protein yield, physiochemical properties and structure of proteins were investigated. As compared with physical treatments (ultrasonication, freeze-thawing and their combination), the enzymatic hydrolysis significantly improved the yield of proteins, because it not only promoted the release of proteins from the inside of pollen, but also released proteins in pollen wall. The proteins extracted by enzymatic hydrolysis method also exhibited better solubility, emulsifying and gelation properties due to the partial hydrolysis of proteins by protease. In addition, when ultrasound was combined with freeze-thawing or enzymatic hydrolysis, it could further improve the yield of proteins and the functional properties of proteins, which was mainly related to the changes of protein structure induced by cavitation effect of ultrasound.
