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Colorimetric Detection of ATP by a Chlorophosphonazo III -based Mg2+ Complex in Aqueous Solution via Indicator Displacement Approach

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Abstract

A simple and effective colorimetric detection of adenosine 5-triphosphate (ATP) in 100% aqueous media was developed based on an indicator displacement approach (IDA). A commercially available dye, Chlorophosphonazo III (CPA), was utilized as the indicator and the ATP detection was achieved using the complex of CPA with Mg2+ in a 2:1 stoichiometric ratio (CPA2-Mg2+) through the regeneration of CPA by the binding of ATP to Mg2+. Upon addition of a series of anions to the CPA2-Mg2+ complex, only the appearance of the solution of the complex with ATP exhibited a color change from blue to purple which can be detected by the naked eye. Moreover, the ATP recognition was not hampered by the presence of other anions. Hence, CPA2-Mg2+ is efficient in ATP highly selective and sensitive colorimetric detection in 100% aqueous media.

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Authors and Affiliations

  1. Faculty of Science and Engineering, Toyo University, 2100 Kujirai, Kawagoe, Saitama, 350-8585, Japan

    Mahiro Shimizu & Yasumasa Fukushima

  2. Research Institute of Industrial Technology, Toyo University, 2100 Kujirai, Kawagoe, Saitama, 350-8585, Japan

    Shunichi Aikawa

Authors
  1. Mahiro Shimizu
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  2. Shunichi Aikawa
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  3. Yasumasa Fukushima
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Contributions

Mahiro Shimizu and Shunichi Aikawa performed absorption measurements and prepared all figures. Yasumasa Fukushima wrote the main manuscript and supervised the study. All authors reviewed the manuscript.

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Correspondence to Yasumasa Fukushima.

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Shimizu, M., Aikawa, S. & Fukushima, Y. Colorimetric Detection of ATP by a Chlorophosphonazo III -based Mg2+ Complex in Aqueous Solution via Indicator Displacement Approach. J Fluoresc 33, 255–260 (2023). https://doi.org/10.1007/s10895-022-03063-5

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