Issue 24, 2022

Ultrathroughput immunomagnetic cell sorting platform

Abstract

High-throughput phenotypic cell sorting is critical to the development of cell-based therapies and cell screening discovery platforms. However, current cytometry platforms are limited by throughput, number of fractionated populations that can be isolated, cell viability, and cost. We present an ultrathroughput microfluidic cell sorter capable of processing hundreds of millions of live cells per hour per device based on protein expression. This device, a next-generation microfluidic cell sorter (NG-MICS), combines multiple technologies, including 3D printing, reversible clamp sealing, and superhydrophobic treatments to create a reusable and user-friendly platform ready for deployment. The utility of such a platform is demonstrated through the rapid isolation of mature natural killer cells from peripheral blood mononuclear cells, for use in CAR-NK therapies at clinically-relevant scale.

Graphical abstract: Ultrathroughput immunomagnetic cell sorting platform

Supplementary files

Article information

Article type
Communication
Submitted
29 Aug 2022
Accepted
04 Nov 2022
First published
07 Nov 2022

Lab Chip, 2022,22, 4822-4830

Ultrathroughput immunomagnetic cell sorting platform

D. N. Philpott, K. Chen, R. S. Atwal, D. Li, J. Christie, E. H. Sargent and S. O. Kelley, Lab Chip, 2022, 22, 4822 DOI: 10.1039/D2LC00798C

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