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Biosynthesis

Caught in the act

YcaO enzymes are able to catalyze a diverse set of reactions and have found industrial applications. New biochemical data provide the first direct evidence for the unified reaction mechanism proposed a decade ago and will inform future enzyme engineering efforts.

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Fig. 1: Reactions and the proposed mechanism of YcaO enzymes.

References

  1. Montalban-Lopez, M. et al. Nat. Prod. Rep. 38, 130–239 (2021).

    Article  CAS  Google Scholar 

  2. Milne, J. C. et al. Biochemistry 38, 4768–4781 (1999).

    Article  CAS  Google Scholar 

  3. Zheng, Y. & Nair, S. K. Nat. Chem. Biol. https://doi.org/10.1038/s41589-022-01141-0 (2022).

    Article  Google Scholar 

  4. Dunbar, K. L., Melby, J. O. & Mitchell, D. A. Nat. Chem. Biol. 8, 569–575 (2012).

    Article  CAS  Google Scholar 

  5. Franz, L., Adam, S., Santos-Aberturas, J., Truman, A. W. & Koehnke, J. J. Am. Chem. Soc. 139, 18158–18161 (2017).

    Article  CAS  Google Scholar 

  6. Schwalen, C. J. et al. J. Am. Chem. Soc. 139, 18154–18157 (2017).

    Article  CAS  Google Scholar 

  7. Mahanta, N., Liu, A., Dong, S., Nair, S. K. & Mitchell, D. A. Proc. Natl Acad. Sci. USA 115, 3030–3035 (2018).

    Article  CAS  Google Scholar 

  8. Gu, W., Zheng, Y., Taras, P., Nair, S. K. & Schmidt, E. W. ACS Chem. Biol. 17, 1215–1225 (2022).

    Article  CAS  Google Scholar 

  9. Dong, S.-H., Liu, A., Mahanta, N., Mitchell, D. A. & Nair, S. K. ACS Cent. Sci. 5, 842–851 (2019).

    Article  CAS  Google Scholar 

Download references

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Correspondence to Jesko Koehnke.

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Koehnke, J. Caught in the act. Nat Chem Biol 19, 7–8 (2023). https://doi.org/10.1038/s41589-022-01180-7

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