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An Acid-triggered Reactive and Enhanced Fluorescent Probe for Selective Detection of Al3+/H+ and its Application in Real Water Samples and Living Cells

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Abstract

A reactive fluorescent “turn-on” probe (di-PIP) with imine-linked dual phenanthro[9,10-d]imidazole luminophore have been conveniently prepared as an Al3+ and H+ dual functional receptor. di-PIP displayed high selectivity and sensitivity towards Al3+ ion in DMF/HEPES accompanied by fluorescence blue-shift and a good linear relationship as well as a low detection limit of 30.5 nmol·L–1, which can root from the synergetic functions of the decomposition reaction of di-PIP promoted by acidic Al3+ and the coordination effect between decomposition product and Al3+. Intriguingly, it was found that hydrogen ion H+ can be sufficient for simulating the fluorescence enhancing of di-PIP. 1H NMR titration and MS analyses for elucidation of the intermediate structure further revealed that the acid-triggered decomposition reaction resulted in the rapid, and sensitive sensing to Al3+ and H+. In addition, the probe di-PIP could be successfully applied to the detection of Al3+ in real water samples, and also utilized to visualize Al3+ and H+ in the living cells.

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Acknowledgements

The work was supported by the National Natural Science Foundation of China (21978140 and 21506106), the Natural Science Foundation of Heilongjiang Province (GZ20210064) and the Qiqihar University Graduate Innovative Scientific Research Project, China (YJSCX2021005). The authors also gratefully acknowledge the financial support by the Heilongjiang Provincial Key Laboratory of Surface Active Agent and Auxiliary (BMHXJKF004 and BMHXJKF005).

Funding

The work was supported by the National Natural Science Foundation of China (21978140 and 21506106), the Natural Science Foundation of Heilongjiang Province (GZ20210064) and the Qiqihar University Graduate Innovative Scientific Research Project, China (YJSCX2021005).

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Authors and Affiliations

  1. Chemistry and Chemical Engineering Institute, Qiqihar University, Qiqihar, Heilongjiang, 161006, China

    Zhigang Li, Bing Zhao, Wei Kan, Fanqiang Bu, Xin Qi, Liyan Wang & Bo Song

  2. Heilongjiang Provincial Key Laboratory of Surface Active Agent and Auxiliary, Qiqihar University, Qiqihar, Heilongjiang, 161006, China

    Bing Zhao, Wei Kan, Liyan Wang & Bo Song

  3. Cadre Ward, First Hospital of Qiqihar City, Qiqihar, Heilongjiang, 161005, China

    Limin Ding

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  1. Zhigang Li
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  2. Bing Zhao
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  3. Wei Kan
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  5. Xin Qi
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  8. Limin Ding
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Bing Zhao, Wei Kan, Liyan Wang, Bo Song, and Limin Ding contributed significantly to write the manuscript and perform the analysis with constructive discussions; Zhigang Li, Fanqiang Bu, and Xin Qi performed the experiments and the data analyses.

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Correspondence to Bing Zhao or Wei Kan.

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Li, Z., Zhao, B., Kan, W. et al. An Acid-triggered Reactive and Enhanced Fluorescent Probe for Selective Detection of Al3+/H+ and its Application in Real Water Samples and Living Cells. J Fluoresc 33, 91–101 (2023). https://doi.org/10.1007/s10895-022-03039-5

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