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Circ_C4orf36 Promotes the Proliferation and Osteogenic Differentiation of BMSCs by Regulating VEGFA

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Abstract

Fracture healing is a complicated process containing the regulation of cellular process. It has been reported that circRNAs are involved in fracture healing. Our study aims to explore the role and mechanism of circ_C4orf36 in fracture healing. Here, we found that the expressions of Circ_C4orf36 and VEGFA were increased during osteoblast differentiation in MC3T3-E1 cells. Circ_C4orf36 overexpression could accelerate the proliferation and migration, as well as osteoblast differentiation in MC3T3-E1 cells, as well as increased ALP activity and osteogenic markers (Runx2, OCN) via upregulating VEGFA expression. Mechanistically, circ_C4orf36 facilitated the expression of VEGFA by recruiting EIF4A3. Taken together, our results elucidated that circ_C4orf6 promoted the migration, proliferation and osteoblast differentiation in BMSCs by upregulating VEGFA, which indicated that circ_C4orf36 might be a potential target in fracture healing treatment.

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Data Availability

All data generated or analyzed during this study are included in this article. The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

CircRNA:

Circular RNA

EIF4A3 :

Eukaryotic initiation factor 4A3

VEGFA :

Vascular endothelial growth factor A

FBS:

Fetal bovine serum

α-MEM:

α-Minimum Essential Medium

PDLSCs:

Periodontal ligament stromal cells

α-MEM:

α-Minimum Essential Medium

PVDF:

Polyvinylidene difluoride

CCK-8:

Cell counting Kit-8

RIP:

RNA Immunoprecipitation

RT-qPCR:

Quantitative real-time PCR

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Authors and Affiliations

  1. Shanxi Medical University, No.86, Xinjian south street, Taiyuan, 030000, Shanxi Province, People’s Republic of China

    Zhi-Min Zhang & Jian-Zhong Huo

  2. Shanxi Medical University, Linfen Hospital, Linfen, 041000, Shanxi Province, People’s Republic of China

    Chun-Xia Huang

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  1. Zhi-Min Zhang
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  2. Chun-Xia Huang
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Contributions

ZMZ: Conceptualization; Writing-original draft; Formal analysis; Methodology; CXH: Data curation; Resources; Investigation; Project administration; Supervision; JZH: Funding acquisition; Software; Visualization; Validation; Writing-review & editing. All authors have read and approved the final version of this manuscript to be published.

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Correspondence to Jian-Zhong Huo.

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Zhang, ZM., Huang, CX. & Huo, JZ. Circ_C4orf36 Promotes the Proliferation and Osteogenic Differentiation of BMSCs by Regulating VEGFA. Biochem Genet 61, 931–944 (2023). https://doi.org/10.1007/s10528-022-10290-9

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  • DOI: https://doi.org/10.1007/s10528-022-10290-9

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