Issue 1, 2023
From the journal:

Natural Product Reports

The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi†

Clara Woodcraft, ORCID logo a   Yit-Heng Chooi ORCID logo *a  and  Indra Roux ORCID logo ‡*a  

* Corresponding authors

a School of Molecular Sciences, The University of Western Australia, Perth, WA 6009, Australia
E-mail: yitheng.chooi@uwa.edu.au, Indra.roux@uwa.edu.au

Abstract

Covering: up to May 2022

Fungal genetics has transformed natural product research by enabling the elucidation of cryptic metabolites and biosynthetic steps. The enhanced capability to add, subtract, modulate, and rewrite genes via CRISPR/Cas technologies has opened up avenues for the manipulation of biosynthetic gene clusters across diverse filamentous fungi. This review discusses the innovative and diverse strategies for fungal natural product discovery and engineering made possible by CRISPR/Cas-based tools. We also provide a guide into multiple angles of CRISPR/Cas experiment design, and discuss current gaps in genetic tool development for filamentous fungi and the promising opportunities for natural product research.

Graphical abstract: The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi

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Article information

DOI
https://doi.org/10.1039/D2NP00055E
Article type
Review Article
Submitted
21 Jul 2022
First published
07 Oct 2022

Nat. Prod. Rep., 2023,40, 158-173

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The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi

C. Woodcraft, Y. Chooi and I. Roux, Nat. Prod. Rep., 2023, 40, 158 DOI: 10.1039/D2NP00055E

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