Issue 85, 2022
From the journal:

Chemical Communications

Boosting artificial nicotinamide cofactor systems†

Ioannis Zachos, ORCID logo a   Samed Güner, ORCID logo a   Arabella Essert,a   Peta Lommesa  and  Volker Sieber ORCID logo *abcd  

* Corresponding authors

a Chair of Chemistry of Biogenic Resources, Technical University of Munich, Campus Straubing for Biotechnology and Sustainability, Schulgasse 16, 94315 Straubing, Germany
E-mail: sieber@tum.de

b Catalysis Research Center, Technical University of Munich, 85748 Garching, Germany

c SynBioFoundry@TUM, Petersgasse 5, 94315 Straubing, Germany

d School of Chemistry and Molecular Biosciences, The University of Queensland, 68 Copper Road, St. Lucia, Queensland 4072, Australia

Abstract

Developing inexpensive nicotinamide cofactor biomimetics to replace the expensive NAD(P)/H cofactors is an ongoing research activity. Here we present mutational studies on a thermostable glucose dehydrogenase from Saccharolobus solfataricus (SsGDH) using a novel set of synthetic cofactors. Furthermore, we show the successful oxidation of a variety of different sugars in the context of cofactor regeneration. This combined approach resulted in an 160-fold improved system compared to the native enzyme with the standard biomimetic BNA+. These findings pave the way towards competitive industrial utilization of artificial cofactor regeneration systems.

Graphical abstract: Boosting artificial nicotinamide cofactor systems

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Article information

DOI
https://doi.org/10.1039/D2CC03423A
Article type
Communication
Submitted
18 Jun 2022
Accepted
28 Sep 2022
First published
05 Oct 2022

Chem. Commun., 2022,58, 11945-11948

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Boosting artificial nicotinamide cofactor systems

I. Zachos, S. Güner, A. Essert, P. Lommes and V. Sieber, Chem. Commun., 2022, 58, 11945 DOI: 10.1039/D2CC03423A

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