Skip to main content
SpringerLink
Log in

Facile synthesis of zinc oxide nanoparticle using algal extract and their antibacterial potential

  • Original Article
  • Published:
Biomass Conversion and Biorefinery Aims and scope Submit manuscript

Abstract

The current study aimed at the synthesis of zinc oxide nanoparticles using water extracts obtained from two different seaweed species Halymenia venusta and Hypnea pannosa. Zinc acetate dehydrate was used as a precursor material in this study. Various characterization techniques were employed to (1) confirm the formation of ZnO nanoparticles (ZnO NPs) and (2) evaluate the morphological properties of the synthesized ZnO NPs such as size and shape. The scanning electron microscopy images of cube-shaped and flake-shaped were obtained for H. pannosa–mediated and H. venusta–mediated ZnO NPs, respectively. The size of the nanoparticles was measured to be 200 nm for Hy. pannosa–mediated ZnO NPs and 200–300 nm for H. venusta–mediated ZnO NPs. Furthermore, these green synthesized ZnO NPs demonstrated strong antibacterial activity against bacterial strains.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12

Similar content being viewed by others

Data availability

Data available on request from the authors.

References

  1. Ashe B (2011) A detail investigation to observe the effect of zinc oxide and silver nanoparticles in biological system. M.Tech dissertation in Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, India.

  2. Buzea C, Pacheco II, Robbie K (2007) Nanomaterials and nanoparticles: sources and toxicity. Biointerphases 2(4):MR17-MR71

  3. Collenburg L, Beyersdorf N, Wiese T, Arenz C, Saied EM, Becker-Flegler KA, Schneider-Schaulies S, Avota E (2017) The activity of the neutral sphingomyelinase is important in T cell recruitment and directional migration. Front Immunol 8:1007

    Article  Google Scholar 

  4. Mohamed AA, Abu-Elghait M, Ahmed NE, Salem SS (2021) Eco-friendly mycogenic synthesis of ZnO and CuO nanoparticles for in vitro antibacterial, antibiofilm and antifungal applications. Biol Trace Elem Res 199(7):2788–2799

    Article  Google Scholar 

  5. Salem SS, EL-Belely EF, Niedbała G, Alnoman MM, Hassan SED, Eid AM, Shaheen TI, Elkelish A, Fouda A, (2020) Bactericidal and In-vitro cytotoxic efficacy of silver nanoparticles (Ag-NPs) fabricated by endophytic actinomycetes and their use as coating for the textile fabrics. Nanomat (Basel) 10(10):2082

    Article  Google Scholar 

  6. Aksoy B, Atakan N, Aksoy HM, Tezel GG, Renda N, Ozkara HA, Onder E (2010) Effectiveness of topical zinc oxide application on hypertrophic scar development in rabbits. Burns 36(7):1027–1035

    Article  Google Scholar 

  7. Mishra PK, Mishra H, Ekielski S, Talegaonkar S, Vaidya B (2017) Zinc oxide nanoparticles: a promising nanomaterial for biomedical applications. Drug Discovery Today 22(12):1825–1834

    Article  Google Scholar 

  8. Prasad ER, Sivakumar SR (2020) Synthesis of zinc oxide nanoparticles (ZnO NPs) via green approach for enhanced antimicrobial activity against human pathogenic bacteria (HPB) strains. Int J Sci Tech Res 9(04):3403–3409

    Google Scholar 

  9. Karthika V, Arumugam A, Gopinath K, Kaleeswarran P, Govindarajan M, Alharbi NS, Kadaikunnan S, Khaled JM, Benelli G (2017) Guazuma ulmifolia bark-synthesized Ag, Au and Ag/Au alloy nanoparticles: photocatalytic potential, DNA/protein interactions, anticancer activity and toxicity against 14 species of microbial pathogens. J Photochem Photobiol B 167:189–199

    Article  Google Scholar 

  10. Devatha CP, Thalla AK, Katte SY (2016) Green synthesis of iron nanoparticles using different leaf extracts for treatment of domestic waste water. J Clean Prod 139:1425–1435

    Article  Google Scholar 

  11. Vijayakumar TS, Mahboob S, Bupesh G, Vasanth S, Al-Ghanim KA, Al-Misned F, Govindarajan M (2020) Facile synthesis and biophysical characterization of egg albumen-wrapped zinc oxide nanoparticles: A potential drug delivery vehicles for anticancer therapy. J Drug Deliv Sci Tech 60:102015

  12. Vinotha V, Yazhiniprabha M, Raj DS, Mahboob S, Al-Ghanim KA, Al-Misned F, Govindarajan M, Vaseeharan B (2020) Biogenic synthesis of aromatic cardamom-wrapped zinc oxide nanoparticles and their potential antibacterial and mosquito larvicidal activity: an effective eco-friendly approach. J Environ Chem Eng 8:6

    Article  Google Scholar 

  13. Makarov VV, Love AJ, Sinitsyna OV, Makarova SS, Yaminsky IV, Taliansky ME, Kalinina NO (2014) Green nanotechnologies: synthesis of metal nanoparticles using plants. Acta Naturae 6(1):35–44

    Article  Google Scholar 

  14. Arya V (2010) Living systems: eco-friendly nanofactories. Dig J Nanomater Bios 5:9–21

    Google Scholar 

  15. Biswas B, Rogers K, Mclaughlin F, Daniels D, Yadav A (2013) Antimicrobial activities of leaf extracts of Guava (Psidium guajava L.) on two Gram-negative and Gram-positive bacteria. Int J Microbiol 2013:746165.

  16. Aleem AA (1993) The marine algae of Alexandria, Egypt. Aleem, A.A. (Ed.), Faculty of science, University of Alexandria, Egypt, pp. 139

  17. Naseer M, Aslam U, Khalid B, Chen B (2020) Green route to synthesize zinc oxide nanoparticles using leaf extracts of Cassia fistula and Melia azadarach and their antibacterial potential. Sci Rep 10(1):1–10

    Article  Google Scholar 

  18. Kalaba MH, Moghannem SA, El-Hawary AS, Radwan AA, Sharaf MH, Shaban AS (2021) Green synthesized ZnO nanoparticles mediated by Streptomyces plicatus: characterizations, antimicrobial and nematicidal activities and cytogenetic effects. Plants 10(9):1760

    Article  Google Scholar 

  19. Abdulwahid KE, Dwaish AS, Dakhil OA (2019) Green synthesis and characterization of zinc oxide nanoparticles from Cladophora glomerata and its antifungal activity against some fungal isolates. Plant Arch 19(2):3527–3532

    Google Scholar 

  20. Chinnasamy C, Tamilselvam P, Karthick B, Sidharth B, Senthilnathan M (2018) Green synthesis, characterization and optimization studies of zinc oxide nano particles using costusigneus leaf extract. Mat Tod: Precede 5(2):6728–6735

    Google Scholar 

  21. Lu J, Ali H, Hurh J, Han Y, Batjikh I, Rupa EJ, Anandapadmanaban G, Park JK, Yang DC (2019) The assessment of photocatalytic activity of zinc oxide nanoparticles from the roots of Codonopsis lanceolata synthesized by one-pot green synthesis method. Optik 184:82–89

    Article  Google Scholar 

  22. Jayappa MD, Ramaiah CK, Kumar MAP, Suresh D, Prabhu A, Devasya RP, Sheikh S (2020) Green synthesis of zinc oxide nanoparticles from the leaf, stem and in vitro grown callus of Mussaenda frondosa L.: characterization and their applications. Appl Nanosci 10(8):3057–3074

    Article  Google Scholar 

  23. Selim YA, Azb MA, Ragab I, Abd El-Azim M (2020) Green synthesis of zinc oxide nanoparticles using aqueous extract of Deverra tortuosa and their cytotoxic activities. Sci Rep 10(1):1–9

    Article  Google Scholar 

  24. Balogun SW, James OO, Sanusi YK, Olayinka OH (2020) Green synthesis and characterization of zinc oxide nanoparticles using bashful (Mimosa pudica), leaf extract: a precursor for organic electronics applications. Appl Sci 2(3):1–8

    Google Scholar 

  25. Sirelkhatim A, Mahmud S, Seeni A, Kaus NHM, Ann LC, Bakhori SKM, Mohamad D (2015) Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nano-Micro Lett 7(3):219–242

    Article  Google Scholar 

  26. Shi LE, Li ZH, Zheng W, Zhao YF, Jin YF, Tang ZX (2014) Synthesis, antibacterial activity, antibacterial mechanism and food applications of ZnO nanoparticles: a review. Food Add Contamin: Part A 31(2):173–186

    Article  Google Scholar 

  27. Yedurkar S, Maurya C, Mahanwar P (2016) Biosynthesis of zinc oxide nanoparticles using Ixora coccinea leaf extract - a green approach. Open J Synthe Theory Appl 5(1):1–14

    Article  Google Scholar 

  28. Bari AR, Shinde MD, Vinita D, Patil LA (2009) Effect of solvents on the particle morphology of nanostructured ZnO. Ind J Pure Appl Phy 47:24–27

    Google Scholar 

  29. Zhou J, Zhao F, Wang Y, Zhang Y, Yang L (2007) Size-controlled synthesis of ZnO nanoparticles and their photoluminescence properties. J Luminescen 122:195–197

    Article  Google Scholar 

  30. Khoshhesab ZM, Sarfaraz M, Asadabad MA (2011) Preparation of ZnO nanostructures by chemical precipitation method. Synth React Inorg Met-Org Nano-Met Chem 41:814–819

    Article  Google Scholar 

  31. Faisal S, Jan H, Shah SA, Shah S, Khan A, Akbar MT, Rizwan M, Jan F, Wajidullah Akhtar N, Khattak A (2021) Green synthesis of zinc oxide (ZnO) nanoparticles using aqueous fruit extracts of Myristica fragrans: their characterizations and biological and environmental applications. ACS Omega 6(14):9709–9722

    Article  Google Scholar 

  32. Akbar N, Aslam Z, Siddiqui R, Shah MR, Khan NA (2021) Zinc oxide nanoparticles conjugated with clinically-approved medicines as potential antibacterial molecules. AMB Exp 11(1):1–16

    Google Scholar 

  33. Rachmawati H, Edityaningrum CA, Mauludin R (2013) Molecular inclusion complex of curcumin–β-cyclodextrin nanoparticle to enhance curcumin skin permeability from hydrophilic matrix gel. AAPS PharmSciTech 14(4):1303–1312

    Article  Google Scholar 

  34. Ebadi M, Zolfaghari MR, Aghaei SS, Zargar M, Shafiei M, Zahiri HS, Noghabi KA (2019) A bio-inspired strategy for the synthesis of zinc oxide nanoparticles (ZnO NPs) using the cell extract of cyanobacterium Nostoc sp. EA03: from biological function to toxicity evaluation. RSC Adv 9(41):23508–23525

    Article  Google Scholar 

  35. Jamdagni P, Khatri P, Rana JS (2018) Green synthesis of zinc oxide nanoparticles using flower extract of Nyctanthes arbor-tristis and their antifungal activity. J King Saud Uni-Sci 30(2):168–175

    Article  Google Scholar 

  36. Alsaggaf MS, Diab AM, ElSaied BE, Tayel AA, Moussa SH (2021) Application of ZnO nanoparticles phycosynthesized with Ulva fasciata extract for preserving peeled shrimp quality. Nanomat 11(2):385

    Article  Google Scholar 

  37. Ramesh M, Anbuvannan M, Viruthagiri GJSAPAM (2015) Green synthesis of ZnO nanoparticles using Solanum nigrum leaf extract and their antibacterial activity. Spectrochimica Acta Part A: Mole Biomole Spec 136:864–870

    Article  Google Scholar 

  38. Ishwarya R, Vaseeharan B, Kalyani S, Banumathi B, Govindarajan M, Alharbi NS, Kadaikunnan S, Al-Anbr MN, Khaled JM, Benelli G (2018) Facile green synthesis of zinc oxide nanoparticles using Ulva lactuca seaweed extract and evaluation of their photocatalytic, antibiofilm and insecticidal activity. J Photochem Photobiol B: Biol 178:249–258

    Article  Google Scholar 

  39. El-Belely EF, Farag MM, Said HA, Amin AS, Azab E, Gobouri AA, Fouda A (2021) Green synthesis of zinc oxide nanoparticles (ZnO-NPs) using Arthrospira platensis (Class: Cyanophyceae) and evaluation of their biomedical activities. Nanomat 11(1):95

    Article  Google Scholar 

  40. Al-Kordy HM, Sabry SA, Mabrouk ME (2021) Statistical optimization of experimental parameters for extracellular synthesis of zinc oxide nanoparticles by a novel haloalaliphilic Alkalibacillus sp. W7. Sci Rep 11(1):1–14

    Article  Google Scholar 

  41. Fouda A, Eid AM, Abdelkareem A, Said HA, El-Belely EF, Alkhalifah DHM, Alshallash KS, Hassan SED (2022) Phyco-synthesized zinc oxide nanoparticles using marine macroalgae, Ulva fasciata Delile, characterization, antibacterial activity, photocatalysis, and tanning wastewater treatment. Catalysts 12(7):756

    Article  Google Scholar 

  42. Anjali KP, Sangeetha BM, Raghunathan R, Devi G, Dutta S (2021) Seaweed mediated fabrication of zinc oxide nanoparticles and their antibacterial. Antifungal and Anticancer Applications ChemistrySelect 6(4):647–656

    Google Scholar 

  43. Hamza MF, Fouda A, Elwakeel KZ, Wei Y, Guibal E, Hamad NA (2021) Phosphorylation of guar gum/magnetite/chitosan nanocomposites for uranium (VI) sorption and antibacterial applications. Molecules 26(7):1920

    Article  Google Scholar 

  44. Roy A, Bulut O, Some S, Mandal AK, Yilmaz MD (2019) Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity. RSC Adv 9(5):2673–2702

    Article  Google Scholar 

  45. Soliman AM, Abdel-Latif W, Shehata IH, Fouda A, Abdo AM, Ahmed YM (2021). Green approach to overcome the resistance pattern of Candida spp. using biosynthesized silver nanoparticles fabricated by Penicillium chrysogenum F9. Biol Trace Elem Res 199(2): 800–811

  46. Adams LK, Lyon DY, Alvarez PJ (2006) Comparative eco-toxicity of nanoscale TiO2, SiO2, and ZnO water suspensions. Water Res 40(19):3527–3532

    Article  Google Scholar 

  47. Brayner R, Ferrari-Iliou R, Brivois N, Djediat S, Benedetti MF, Fiévet F (2006) Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium. Nano Lett 6(4):866–870

    Article  Google Scholar 

  48. Zhang L, Ding Y, Povey M, York D (2008) ZnO nanofluids—a potential antibacterial agent. Prog Nat Sci 18(8):939–944 79

  49. Lipovsky A, Nitzan Y, Gedanken A, Lubart R (2011) Antifungal activity of ZnO nanoparticles—the role of ROS mediated cell injury. Nanotechnology 22(10):105101

    Article  Google Scholar 

  50. Sawai J, Shoji S, Igarashi H, Hashimoto A, Kokugan T, Shimizu M, Kojima H (1998) Hydrogen peroxide as an antibacterial factor in zinc oxide powder slurry. J Ferment Bioeng 86(5):521–522

    Article  Google Scholar 

  51. Zhang L, Jiang Y, Ding Y, Povey M, York D (2007) Investigation into the antibacterial behaviour of suspensions of ZnO nanoparticles (ZnO nanofuids). J Nanopart Res 9(3):479–489

    Article  Google Scholar 

  52. Dineshkumar R, Sowndariya M, Kalaiselvi S, Israth Rehana G, Durai Murugan M, Marykutty A, Moovendhan M, Kavisri M (2022) Effective removal of lead (Pb) by natural biosorbent marine microalgae (Dunaliella salina) through batch experiment. Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-021-02260-9

    Article  Google Scholar 

  53. Kavisri M, Marykutty A, Prabakaran G, Elangovan M, Moovendhan M (2021) Phytochemistry, bioactive potential and chemical characterization of metabolites from marine microalgae (Spirulina platensis) biomass. Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-021-01689-2

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. PG & Research Department of Botany, V.O.Chidambaram College, Tuticorin, 628008, Tamilnadu, India

    R. Rabecca &  A. Doss

  2. Department of Botany, S.T.Hindu College, Nagercoil, 629002, Tamilnadu, India

    V. Mary Kensa,  S. Iswarya &  N. Mukeshbabu

  3. PG & Research Department of Zoology, V.O.Chidambaram College, Tuticorin, 628008, Tamilnadu, India

    R.P. Praveen Pole

  4. Department of Chemical and Biochemical Engineering, Dongguk University, Seoul, Republic of Korea

    K. Iyappan

Authors
  1. R. Rabecca
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Doss
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. Mary Kensa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Iswarya
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. Mukeshbabu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. R.P. Praveen Pole
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. K. Iyappan
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

RR, AD, and VMK: conceptualization, methodology, formal analysis, investigation, writing—original draft, writing—review and editing, visualization. SI, NMB, RPPP, and KI: methodology, formal analysis, investigation, and manuscript proof reading.

Corresponding author

Correspondence to A. Doss.

Ethics declarations

Ethical approval

In this study, animal experiment was not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare no competing interests.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

R. Rabecca, A. Doss, V. Mary Kensa et al. Facile synthesis of zinc oxide nanoparticle using algal extract and their antibacterial potential. Biomass Conv. Bioref. (2022). https://doi.org/10.1007/s13399-022-03275-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13399-022-03275-6

Keywords

Search

Navigation