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Biochemical changes induced in the maize leaf sheath upon inoculation with Rhizoctonia solani incitant of banded leaf and sheath blight

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Abstract

Banded leaf and sheath blight (BLSB) of maize is an emerging disease in the northwest region of India. The present study investigates the potential role of defense-related enzymes in imparting resistance against Rhizoctonia solani in seven inbreds, viz., LMDR-2, CM-143, CM-600, LM-11, LM-12, LM-13 and LM-14, and four maize hybrids, viz., PMH1, PMH2, PMH4 and JH3459. The enzyme activities were determined from the leaf sheaths collected at 0, 24, 48, 72, 96, 120 and 144 h post-inoculation. The results indicated a spike in the expression of phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), peroxidase (POX) and catalase (CAT) at 72 h post-inoculation following pathogen challenge in different maize inbreds/hybrids. The moderately resistant group (LMDR-2 and JH3459) showed significantly higher activities than the susceptible group (LM-11, LM-12, CM-600, PMH4 and PMH2). A strong positive correlation was observed between different enzymes at 72 and 96 h after inoculation. Lesion length showed negative correlation with the activities of enzymes studied. Our results indicated that PAL, PPO, POX and CAT played key roles in providing BLSB resistance in maize inbreds/hybrids.

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The data generated and/or analyzed during the present study are attached as supporting information and can also be obtained from the corresponding author on request.

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Funding

The research was fully supported by Indian Council of Agricultural Research, New Delhi—Extramural grant ‘Characterization, population dynamics and management of Rhizoctonia in different cropping systems’ to HK and MSH.

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Authors and Affiliations

  1. University Institute of Agricultural Sciences, Chandigarh University, Mohali, Punjab, India

    Sanjay Kumar

  2. Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, India

    Harleen Kaur

  3. Department of Plant Pathology, Punjab Agricultural University, Ludhiana, India

    Mandeep Singh Hunjan

  4. Department of Biochemistry, Punjab Agricultural University, Ludhiana, India

    Kamaljit Kaur

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  1. Sanjay Kumar
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  2. Harleen Kaur
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  4. Kamaljit Kaur
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All authors contributed equally to this study. Material preparation, data collection and statistical analysis were performed by Sanjay Kumar. All authors prepared the manuscript.

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Correspondence to Sanjay Kumar or Harleen Kaur.

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Communicated by M. R. Simon.

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Kumar, S., Kaur, H., Hunjan, M.S. et al. Biochemical changes induced in the maize leaf sheath upon inoculation with Rhizoctonia solani incitant of banded leaf and sheath blight. CEREAL RESEARCH COMMUNICATIONS 51, 413–423 (2023). https://doi.org/10.1007/s42976-022-00310-0

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