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Production of cassava peel-based xylooligosaccharides using endo-1,4-β-xylanase from Trichoderma longibrachiatum: the effect of alkaline pretreatment

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Abstract

The lignocellulosic residue cassava peel is an unexplored source of bioactive compounds, such as hemicellulose-based xylooligosaccharides (XOS) that present prebiotic properties. In this sense, this work aimed to produce XOS from cassava peels that were pretreated with NaOH (xylan extraction and lignin removal) followed by enzymatic hydrolysis (endo-1–4-β-xylanase). The cassava peels were pretreated sequentially: starch removal, alkaline hydrolysis, and enzymatic hydrolysis. The aqueous-mechanical reduction and sieving for 15 cycles removed ≈16% of starch (iodometric method). The alkaline pretreatments were carried out with 2, 4, and 6% (w/v) NaOH, 1:100/solid:liquid at 121 °C and 1.1 bar for 30 or 60 min. The enzymatic kinetics was evaluated with enzyme concentration at 0.5, 1.5, and 3.0% (v/v). The highest alkaline hydrolysis reached 34.20% of lignin removal (2% (w/v) NaOH for 30 min). The highest XOS yield was 396.5 mg XOS/g xylan after 48 h and 3.0% enzyme concentration. Regarding the mass balance, from 300 g of cassava peels (an agroindustrial residue), it is possible to obtain up to 3.27% XOS. Therefore, the fractionation of hemicellulose from cassava peels was technically viable from the concepts of biorefinery and bioeconomy, being one of the first researches to approach the extraction of xylan from cassava peel to obtain XOS through an enzymatic route with a highly promising yield.

Highlights

• The high starch content is a drawback to producing cassava peel-based xylooligosaccharides.

• Delignification and extraction of xylan from cassava peels by alkaline hydrolysis were performed.

• Xylooligosaccharides were produced by enzymatic hydrolysis with endo-1–4-β-xylanase.

• The highest yield of cassava peel-based xylooligosaccharides, already reported.

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Acknowledgements

The authors acknowledge the financial support of Foundation for the Research and Innovation Support Foundation of Santa Catarina State (FAPESC) for the founding Programa de Apoio a Núcleos Emergentes (PRONEM) 2020TR731 and by the scholarship of co-author Eduardo Zanella. Many thanks also for Coordination for the Improvement of Higher Education Personnel (CAPES)—Institutional Program for Internationalization (PRINT), project numbers 88887.310560/2018-00 and 88887.310727/2018-00.

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Authors and Affiliations

  1. Integrated Biological Engineering Laboratory, Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina (UFSC), Campus Trindade, postal code 476, Florianopolis, Santa Catarina, 88010-970, Brazil

    William Rogoski, Gabriela Nayana Pereira, Karina Cesca, Moisés Amancio da Silva, Débora de Oliveira & Cristiano José de Andrade

  2. Yeast Molecular Biology and Biotechnology Laboratory, Department of Biochemistry, Federal University of Santa Catarina (UFSC), Florianopolis, Santa Catarina, Brazil

    Eduardo Zanella & Boris U. Stambuk

  3. Bioprocess and Metabolic Engineering Laboratory, School of Food Engineering, Department of Food Technology Engineering, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Patrícia F. Ávila & Rosana Goldbeck

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  1. William Rogoski
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  2. Gabriela Nayana Pereira
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Contributions

William Rogoski: conceptualization, methodology, software, writing—original draft, writing—article and editing, and formal analysis; Gabriela Nayana Pereira: formal analysis and conceptualization; Karina Cesca: conceptualization, project administration; Moisés Amancio da Silva: formal analysis; Eduardo Zanella: formal analysis; Boris U. Stambuk: formal analysis; Patrícia F. Ávila: formal analysis; and Rosana Goldbeck: formal analysis. Débora de Oliveira: term, supervision, funding acquisition; Cristiano José de Andrade: term, supervision, funding acquisition.

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Correspondence to Cristiano José de Andrade.

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Rogoski, W., Pereira, G.N., Cesca, K. et al. Production of cassava peel-based xylooligosaccharides using endo-1,4-β-xylanase from Trichoderma longibrachiatum: the effect of alkaline pretreatment. Biomass Conv. Bioref. (2022). https://doi.org/10.1007/s13399-022-03287-2

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