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Preventive Effect and Mechanism of Anthocyanins from Aronia Melanocarpa Elliot on Hepatic Fibrosis Through TGF-β/Smad Signaling Pathway

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Abstract

In order to explore the effect and mechanism of Aornia mealnocarpa Elliot anthocyanins (AMA) at the cellular level on hepatic fibrosis (HF), molecular docking, RT-PCR and Western Blotting were used to explore the molecular mechanism and the effects of different doses AMA on HSC-T6 cells by TGF-β1 induction. The results showed that the binding energy of anthocyanins on TGF-β1 (PDB ID: 3KFD) was in the range of −9.5 to 8.6 kcal/mol, with good low energy parameters and binding positions. AMA could effectively inhibit the expressions of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and total serum bilirubin (TSB), and improved the expressions of total protein (TP) and albumin (ALB). RT-PCR and Western bloting results showed that AMA could inhibited the secretion of inflammatory cytokines IL-1, IL-6, TNF-α and COX-2, and inhibit the expression of TGF-β1, P-Smad2, α-SMA and Collagen I in TGF-β /Smad signaling pathway. This study revealed the AMA’s inhibition effects and mechanism of malignant biological behavior of HSC-T6 cells, in order to provide theoretical basis for the prevention and treatment of HF by Aronia melanocarpa Elliot.

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Funding

This research was supported by Scientific Research Project of the Education Department of Liaoning Province (No. LJC202011), Liaoning provincial key R & D project (No. 2020JH2/10300114) and “Liaoning Talent Program” Project in Liaoning Province (No. XLYC1902081).

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  1. These authors contributed equally: Ruobing Hao, Jun Gao

Authors and Affiliations

  1. School of Life Science, Liaoning University, Shenyang, Liaoning, 110036, China

    Ruobing Hao, Hongwei Liu, Chenjuan Zhang, Jinpeng Huang & Jie Wei

  2. Liaoning Forestry Academy, Shenyang, 110032, China

    Jun Gao & Jungang Fan

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  1. Ruobing Hao
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  2. Jun Gao
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  3. Hongwei Liu
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  4. Chenjuan Zhang
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  5. Jinpeng Huang
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  6. Jungang Fan
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  7. Jie Wei
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R.H. wrote the paper, J.G., H.L., C.Z., and J.F. performed the research; J.W. analyzed the data and designed the research study.

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Correspondence to Jie Wei.

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Hao, R., Gao, J., Liu, H. et al. Preventive Effect and Mechanism of Anthocyanins from Aronia Melanocarpa Elliot on Hepatic Fibrosis Through TGF-β/Smad Signaling Pathway. Cell Biochem Biophys 80, 737–745 (2022). https://doi.org/10.1007/s12013-022-01079-z

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