Abstract
The direct application of heavy metal- and quaternary ammonium-based antibacterial agents can cause inconvenience such as irritation, short-term applicability, discoloration of the tissue, and environmental concerns. The immobilization of these agents on montmorillonite (Mnt) was expected to diminish these effects by hindering direct contact of the ions with the target tissues. The objective of the present study was, therefore, to prepare inorgano(I)- and organo(O)-montmorillonites (I/O-Mnt) and to determine their potential uses in such biomedical applications. Na-montmorillonite (Mnt-Na) was modified by hydrothermal and microwave irradiation methods using Cu2+/Zn2+, and quaternary ammonium and/or anionic surfactants. The effect of the structures formed by immobilization on Mnt surfaces on antibacterial activity was investigated. Quaternary ammonium surfactants were cetyltrimethyl ammonium bromide (CTAB) with a linear alkyl chain, cetylpyridinium chloride (CPC) with a single aromatic ring, and benzethonium chloride (BZT) with double aromatic rings. N-lauroyl sarcosinate (SR) was the anionic surfactant. The samples were subjected to thermogravimetric (TGA) and scanning electron microscopy (SEM) analyses. Desorption tests showed that the antibacterial efficacy against Streptococcus mutans stemmed from I/O-Mnt and not from the ions released from the material surfaces to the aqueous phase. The results of the antibacterial studies showed that the existence of a linear alkyl chain and a double aromatic ring were the structural factors causing the greatest antibacterial effect. The time-kill tests revealed that Mnt-CTA, Mnt-BZT, and Mnt-CP-SR were effective against Streptococcus mutans within 5 min of contact. With the new findings, they were identified as possible selective and potent bactericidal agents and promising candidates for biomedical applications.
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References
ASTM E2149-13a Standard Test Method for Determining the Antimicrobial Activity of Antimicrobial Agents Under Dynamic Contact Conditions (2013). ASTM International. West Conshohocken, Pennsylvania, USA.
Atia, A. A. (2008). Adsorption of chromate and molybdate by cetylpyridinium bentonite. Applied Clay Science, 41, 73–84. https://doi.org/10.1016/j.clay.2007.09.011
Bernardi, A., & Teixeira, C. S. (2015). The properties of chlorhexidine and undesired effects of its use in endodontics. Quintessence International, 46(7), 575–582. https://doi.org/10.3290/j.qi.a33934
Bescos, R., Ashworth, A., Cutler, C., Brookes, Z. L., Belfeld, L., Rodiles, A., Agustench, P. C., Farnham, G., Liddle, L., Burleigh, M., White, D., Easton, C., & Hickson, M. (2020). Effects of chlorhexidine mouthwash on the oral microbiome. Scientific Reports, 10, 5254. https://doi.org/10.1038/s41598-020-61912-4
Brindley, G. W., & Moll Jr., W. F. (1965). Complexes of natural and synthetic Ca-montmorillonites with fatty acids (clay-organ studies-ix). American Mineralogist, 50, 1355–1370.
Chen, K., Ye, W., Cai, S., Huang, L., Zhong, T., Chen, L., & Wang, X. (2016). Green antimicrobial coating based on quaternised chitosan/ organic montmorillonite/Ag NPs nanocomposites. Journal of Experimental Nanoscience, 11(17), 1360–1371. https://doi.org/10.1080/17458080.2016.1227095
CLSI - Clinical and Laboratory Standards Institute. (2015). CLSI document M07-A10 methods for dilution of antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard—10th Edition. Clinical and Laboratory Standards Institute.
Cross, S. E., Kreth, J., Zhu, L., Qi, F., Pelling, A. E., Shi, W., & Gimzewski, J. K. (2006). Atomic force microscopy study of the structure–function relationships of the biofilm-forming bacterium Streptococcus mutans. Nanotechnology, 17(4), S1–S7. https://doi.org/10.1088/0957-4484/17/4/001
Escribano, M., Herrera, D., Morante, S., Teughels, W., Quirynen, M., & Sanz, M. (2010). Efficacy of a low-concentration chlorhexidine mouth rinse in non-compliant periodontitis patients attending a supportive periodontal care programme: a randomized clinical trial. Journal of Clinical Periodontology, 37(3), 266–275. https://doi.org/10.1111/j.1600-051X.2009.01521.x
Gilbert, P., & McBain, A. J. (2003). Potential impact of increased use of biocides in consumer products on prevalence of antibiotic resistance. Clinical Microbiology Reviews, 16(2), 189–208. https://doi.org/10.1128/CMR.16.2.189-208.2003
Gilbert, P., & Moore, L. E. (2005). A review, cationic antiseptics: diversity of action under a common epithet. Journal of Applied Microbiology, 99(4), 703–715. https://doi.org/10.1111/j.1365-2672.2005.02664.x
Güven, Y., Ustun, N., Tuna, E. B., & Aktoren, O. (2019). Antimicrobial effect of newly formulated toothpastes and a mouthrinse on specific microorganisms: An in vitro study. European Journal of Dentistry, 13(2), 172–177. https://doi.org/10.1055/s-0039-1695655
Herrera, P., Burghardt, R. C., & Phillips, T. D. (2000). Adsorption of Salmonella enteritidis by cetylpyridinium-exchanged montmorillonite clays. Veterinary Microbiology, 74(3), 259–272. https://doi.org/10.1016/s0378-1135(00)00157-7
Hsu, S.-H., Wang, M.-C., & Lin, J.-J. (2012). Biocompatibility and antibacterial evaluation of montmorillonite /chitosan nanocomposites. Applied Clay Science, 56, 53–62. https://doi.org/10.1016/j.clay.2011.09.016
Jiao, L., Lin, F., Cao, S., Wang, C., Wu, H., Shu, M., & Hu, C. (2017). Preparation, characterization, antimicrobial and cytotoxicity studies of copper/zinc loaded montmorillonite. Journal of Animal Science and Biotechnology, 8(27), 1–7. https://doi.org/10.1186/s40104-017-0156-6
Jiao, L. F., Ke, Y. L., Xiao, K., Song, Z. H., Lu, J. J., & Hu, C. H. (2015). Effects of zinc-exchanged montmorillonite with different zinc loading capacities on growth performance, intestinal microbiota, morphology and permeability in weaned piglets. Applied Clay Science, 11, 40–43. https://doi.org/10.1016/j.clay.2015.04.012
Lemos, J. A., Palmer, S. R., Zeng, L., Wen, Z. T., Kajfasz, J. K., Freires, I. A., Abranches, J., & Brady, L. J. (2019). The biology of Streptococcus mutans. Microbiology Spectrum, 7(1), 10.1118. https://doi.org/10.1128/microbiolspec.GPP3-0051-2018
Ma, Y.-L., Yang, B., & Xie, L. (2010). Adsorptive property of Cu2+-ZnO/cetylpridinium-montmorillonite complexes for pathogenic bacterium in vitro. Colloids and Surfaces B: Biointerfaces, 79(2), 390–396. https://doi.org/10.1016/j.colsurfb.2010.05.001
Makvandi, P., Ghaemy, M., Ghadiri, A. A., & Mohseni, M. (2015). Photocurable, antimicrobial quaternary ammonium–modified nanosilica. Journal of Dental Research, 94(10), 1401–1407. https://doi.org/10.1177/0022034515599973
Malachová, K., Praus, P., Rybková, Z., & Kozák, O. (2011). Antibacterial and antifungal activities of silver, copper and zinc montmorillonites. Applied Clay Science, 53(4), 642–645. https://doi.org/10.1016/j.clay.2011.05.016
Özdemir, G., Hoşgör-Limoncu, M., & Yapar, S. (2010). The antibacterial effect of heavy metal and cetylpridinium-exchanged montmorillonites. Applied Clay Science, 48(3), 319–323. https://doi.org/10.1016/j.clay.2010.01.001
Özdemir, G., & Yapar, S. (2020). Preparation and characterization of copper and zinc adsorbed cetylpyridinium and N-lauroylsarcosinate intercalated montmorillonites and their antibacterial activity. Colloids and Surfaces B: Biointerfaces, 188, 110791. https://doi.org/10.1016/j.colsurfb.2020.110791
Özdemir, G., Yapar, S., & Hoşgör-Limoncu, M. (2013). Preparation of cetylpyridinium montmorillonite for antibacterial applications. Applied Clay Science, 72, 201–205. https://doi.org/10.1016/j.clay.2013.01.010
Pupe, C. G., Villardi, M., Rodrigues, C. R., Rocha, H. V. A., Maia, L. C., de Sousa, V. P., & Cabral, L. M. (2011). Preparation and evaluation of antimicrobial activity of nanosystems for the control of oral pathogens Streptococcus mutans and Candida albicans. International Journal of Nanomedicine, 6, 2581–2590. https://doi.org/10.2147/IJN.S25667
Rubin, J. E. (2013). Antibacterial susceptibility testing methods and interpretation of results. In S. Giguére, J. F. Prescott, & P. M. Dowling (Eds.), Antimicrobial Therapy in Veterinary Medicine (pp. 11–19). John Wiley and Sons Inc. https://doi.org/10.1002/9781118675014.ch2
Song, W., & Ge, S. (2019). Application of antimicrobial nanoparticles in dentistry. Molecules, 24(6), 1033. https://doi.org/10.3390/molecules24061033
Türker, S., Yarza, F., Sánchez, R. M. T., & Yapar, S. (2017). Surface and interface properties of benzethoniumchloride-montmorillonite. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 520, 817–825. https://doi.org/10.1016/j.colsurfa.2017.02.019
Xi, Y., Martens, W., He, H., & Frost, R. L. (2005). Thermogravimetric analysis of organoclays intercalated with the surfactant octadecyltrimethylammonium bromide. Journal of Thermal Analysis and Calorimetry, 81(1), 91–97. https://doi.org/10.1007/s10973-005-0750-2
Yapar, S., Ateş, M., & Özdemir, G. (2017). Preparation and characterization of sodium lauroyl sarcosinate adsorbed on cetylpyridinium-montmorillonite as a possible antibacterial agent. Applied Clay Science, 150, 16–22. https://doi.org/10.1016/j.clay.2017.08.025
Yapar, S., Özdemir, G., Solarte, A. M. F., & Sánchez, R. M. T. (2015). Surface and interface properties of lauroyl sarcosinate-adsorbed CP+-montmorillonite. Clays and Clay Minerals, 63(2), 110–118. https://doi.org/10.1346/CCMN.2015.0630203
Zhao, D., Zhou, J., & Liu, N. (2006). Preparation and characterization of Mingguang palygorskite supported with silver and copper for antibacterial behavior. Applied Clay Science, 33(3-4), 161–170. https://doi.org/10.1016/j.clay.2006.04.003
Zhu, J., He, H., Guo, J., Yang, D., & Xie, X. (2003). Arrangement models of alkylammonium cations in the interlayer of HDTMA + pillared montmorillonites. Chinese Science Bulletin, 48, 368–372. https://doi.org/10.1007/BF03183232
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The authors gratefully acknowledge the support of Scientific Research Projects of Ege University (BAP) through the project number FGA-2019-20715 and proofreading assistance to Enago, Crimson Interactive Inc, New York.
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Şahiner, A., Özdemir, G., Bulut, T.H. et al. Synthesis and Characterization of Non-leaching Inorgano- and Organo-montmorillonites and their Bactericidal Properties Against Streptococcus Mutans. Clays Clay Miner. 70, 481–491 (2022). https://doi.org/10.1007/s42860-022-00198-1
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DOI: https://doi.org/10.1007/s42860-022-00198-1