Chemical control over the conversion between bicyclic and polycyclic terpenes by fungal bifunctional terpene synthases

Xinye Wang; Zhixin Wang; Guoliang Zhu; Lan Jiang; Weiyan Zhang; Yiyi Huang; Zhanren Cong; Yi-Lei Zhao; Jian-He Xu; Tom Hsiang; Lixin Zhang; Qi Chen; Xueting Liu

doi:10.1039/D2CC03644D

Chemical control over the conversion between bicyclic and polycyclic terpenes by fungal bifunctional terpene synthases†

Xinye Wang,^a Zhixin Wang,^a Guoliang Zhu,^a Lan Jiang,^a Weiyan Zhang,^a Yiyi Huang,^a Zhanren Cong,^a Yi-Lei Zhao,

^b Jian-He Xu,^a Tom Hsiang,^c Lixin Zhang,^a Qi Chen*^a and Xueting Liu

*^a

Author affiliations

* Corresponding authors

^a State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
E-mail: chenq@ecust.edu.cn, liuxueting@ecust.edu.cn

^b State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, MOE-LSB & MOE-LSC, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China

^c School of Environmental Sciences, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada

Abstract

Fungal bifunctional terpene synthases (BFTSs) reportedly associate with a series of new skeletons of di/sesterterpenes. However, the molecular mechanisms underlying the variabilities in the ring system of BFTS-catalyzed products are not well understood. In this study, we identified a key site, S89/L89, that controls the conversion between bicyclic and polycyclic terpene skeletons catalyzed by two BFTSs, BsPS and FoFS. Our analysis revealed that a mutation on site 89 in the BFTSs alters the carbocation transportation pathway and redirects the competing reactions for previously unreported terpenes.

Chemical Communications

Chemical control over the conversion between bicyclic and polycyclic terpenes by fungal bifunctional terpene synthases†

Abstract

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Chemical control over the conversion between bicyclic and polycyclic terpenes by fungal bifunctional terpene synthases

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