NOAH-(15N/13C)-CEST NMR supersequence for dynamics studies of biomolecules

Rodrigo Cabrera Allpas; Alexandar L. Hansen; Rafael Brüschweiler

doi:10.1039/D2CC02015G

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NOAH-(¹⁵N/¹³C)-CEST NMR supersequence for dynamics studies of biomolecules†

Rodrigo Cabrera Allpas,

^a Alexandar L. Hansen

^b and Rafael Brüschweiler

*^abc

Author affiliations

* Corresponding authors

^a Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
E-mail: bruschweiler.1@osu.edu

^b Campus Chemical Instrument Center, The Ohio State University, Columbus, Ohio 43210, USA

^c Department of Biological Chemistry and Pharmacology, The Ohio State University, Columbus, Ohio 43210, USA

Abstract

An NMR supersequence is introduced for the rapid acquisition of ¹⁵N-CEST and methyl-¹³C-CEST experiments in the same pulse sequence for applications to proteins. The high sensitivity and accuracy allows the simultaneous quantitative characterization of backbone and side-chain dynamics on the millisecond timescale ideal for routine screening for alternative protein states.

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Article information

DOI: https://doi.org/10.1039/D2CC02015G
Article type: Communication
Submitted: 08 Apr 2022
Accepted: 31 May 2022
First published: 29 Jul 2022
This article is Open Access

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Chem. Commun., 2022,58, 9258-9261

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NOAH-(¹⁵N/¹³C)-CEST NMR supersequence for dynamics studies of biomolecules

R. Cabrera Allpas, A. L. Hansen and R. Brüschweiler, Chem. Commun., 2022, 58, 9258 DOI: 10.1039/D2CC02015G

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