Issue 17, 2022
From the journal:

Analyst

CRISPR/Cas12a-based fluorescence immunoassay: combination of efficient signal generation with specific molecule recognition†

Xi Li,a   Yuyuan Zhang,a   Qiang Liu,a   Yan Jin ORCID logo a  and  Baoxin Li ORCID logo *a  

* Corresponding authors

a Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China
E-mail: libaoxin@snnu.edu.cn

Abstract

Using the DNA activator of Cas to label the secondary antibody of traditional ELISA, the CRISPR/Cas12a-based fluorescence immunoassay was presented. The sensing strategy ingeniously combines the efficient signal generation of the CRISPR/Cas12a system with antigen–antibody-specific recognition. This work provides an alternative strategy to expand the application of the CRISPR/Cas system for the detection of non-nucleic acid targets.

Graphical abstract: CRISPR/Cas12a-based fluorescence immunoassay: combination of efficient signal generation with specific molecule recognition

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Article information

DOI
https://doi.org/10.1039/D2AN01048H
Article type
Communication
Submitted
29 Jun 2022
Accepted
27 Jul 2022
First published
28 Jul 2022

Analyst, 2022,147, 3833-3837

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CRISPR/Cas12a-based fluorescence immunoassay: combination of efficient signal generation with specific molecule recognition

X. Li, Y. Zhang, Q. Liu, Y. Jin and B. Li, Analyst, 2022, 147, 3833 DOI: 10.1039/D2AN01048H

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