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Sublingual microcirculation: comparison between the 415 nm blue light and 520 nm green light of sidestream dark field videomicroscopes

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Abstract

Green light with a wavelength of 520 nm is commonly used in sidestream dark field (SDF) video microscopes for sublingual microcirculation assessment in clinical practice. However, blue light could obtain a clearer microcirculatory image due to a higher light absorption coefficient of hemoglobin. The aim of this study was to compare the sublingual microcirculatory image quality acquisition and related microcirculatory parameters between 520 nm green light and 415 nm blue light probes in the SDF device named MicroSee V100. Sublingual microcirculation films from twenty-one healthy volunteers were prospectively collected by blue light and green light probes, and only one video of each wavelength was recorded and analyzed in each volunteer. Moreover, 200 sublingual microcirculation films (100 by blue light probe and 100 by green light probe) of ICU patients were retrospectively scored for microcirculation image quality. Compared to green light, an increase in the perfused vessel density (paired t test, increased by 4.6 ± 4.7 mm/mm2, P < 0.0001) and total vessel density (paired t test, increased by 5.1 ± 4.6 mm/mm2, P < 0.0001) was observed by blue light in the healthy volunteers. The blue light probe had a significantly lower rate of unacceptable films than the green light probe in the 200 films of ICU patients (10/100 vs. 39/100, P < 0.0001). Blue light provides a higher microcirculatory vessel density and image quality than the existing SDF probe using green light.

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Acknowledgements

This work was supported by Peking Union Medical College Hospital of Medical Novel Medical Technology Project (No. XJS20190210), Excellence Program of Key Clinical Specialty of Critical Care Medicine in Beijing.

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Authors and Affiliations

  1. Department of Critical Care Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Beijing, China

    Bo Liu, Huaiwu He, Xiaokai Feng, Siyi Yuan & Yun Long

  2. Department of Critical Care Medicine, affiliated hospital of Ji Ning Medical University, Jining, China

    Bo Liu

  3. ICU, Hebi People’s Hospital of Henan Province, Zhengzhou, China

    Xiaokai Feng

  4. Department of Intensive Care, Erasmus MC University Hospital Rotterdam, Rotterdam, Netherlands

    Huaiwu He, Şakir Akin & Can Ince

  5. Department of Intensive Care, Haga Teaching Hospital, The Hague, The Netherlands

    Şakir Akin

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  1. Bo Liu
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  2. Huaiwu He
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  7. Can Ince
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Correspondence to Huaiwu He.

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Dr. Ince is the CSO of Active Medical BV, Leiden The Netherlands, a company that provides education, services, hardware and software related to clinical microcirculation. There are no financial disclosures for other authors.

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Liu, B., He, H., Feng, X. et al. Sublingual microcirculation: comparison between the 415 nm blue light and 520 nm green light of sidestream dark field videomicroscopes. J Clin Monit Comput 37, 297–302 (2023). https://doi.org/10.1007/s10877-022-00891-5

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  • DOI: https://doi.org/10.1007/s10877-022-00891-5

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