Abstract
Circular RNAs (circRNAs) play important roles in human diseases, including infantile pneumonia. In this article, we aimed to investigate the functions of circ-BICC1 in lipopolysaccharide (LPS)-induced injury of WI-38 cells. Quantitative real-time polymerase chain reaction (qRT-PCR) assay was performed for the levels of circ-BICC1, BICC1, microRNA-338-3p (miR-338-3p), and myeloid differentiation primary response 88 (MYD88). Cell Counting Kit-8 (CCK-8) assay, 5-ethynyl-2’-deoxyuridine (EdU) assay, and flow cytometry analysis were conducted to evaluate cell viability, proliferation, and apoptosis, respectively. Enzyme-linked immunosorbent assay (ELISA) kits were used for the concentrations of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). The levels of oxidative stress markers were detected with commercial kits. Dual-luciferase reporter assay was adopted to analyze the interaction between circ-BICC1 and miR-338-3p, as well as MYD88 and miR-338-3p. Western blot assay was employed for the protein level of MYD88. Circ-BICC1 level was increased in pneumonia patients’ blood samples and LPS-treated WI-38 cells. LPS treatment suppressed WI-38 cell viability and promoted cell apoptosis, inflammation, and oxidative stress. Circ-BICC1 knockdown reversed the effect of LPS-induced WI-38 cell injury. For mechanism analysis, circ-BICC1 could function as the sponge for miR-338-3p and miR-338-3p inhibition reversed the effect of circ-BICC1 knockdown on LPS-induced WI-38 cell injury. MYD88 was identified as the target of miR-338-3p. MiR-338-3p overexpression relieved LPS-induced injury of WI-38 cells, while the impact was abolished by elevating MYD88. Circ-BICC1 silencing remitted LPS-triggered WI-38 cell damage by adsorbing miR-338-3p and regulating MYD88.
Similar content being viewed by others
Data Availability
Please contact the correspondence author for the data request.
Code Availability
N/A.
References
Abd-El-Fattah AA, Sadik NA, Shaker OG, Aboulftouh ML (2013) Differential microRNAs expression in serum of patients with lung cancer, pulmonary tuberculosis, and pneumonia. Cell Biochem Biophys 67:875–884
Ahmad A, Shameem M, Husain Q (2012) Relation of oxidant-antioxidant imbalance with disease progression in patients with asthma. Ann Thorac Med 7:226–232
Bertrams W, Griss K, Han M, Seidel K, Klemmer A, Sittka-Stark A, Hippenstiel S, Suttorp N, Finkernagel F, Wilhelm J, Greulich T, Vogelmeier CF, Vera J, Schmeck B (2020) Transcriptional analysis identifies potential biomarkers and molecular regulators in pneumonia and COPD exacerbation. Sci Rep 10:241
Chen LL (2016) The biogenesis and emerging roles of circular RNAs. Nat Rev Mol Cell Biol 17:205–211
Chen CH, Wen HJ, Chen PC, Lin SJ, Chiang TL, Hsieh IC, Guo YL (2012) Prenatal and postnatal risk factors for infantile pneumonia in a representative birth cohort. Epidemiol Infect 140:1277–1285
Du J, Xu J, Chen J, Liu W, Wang P, Ye K (2020) circRAE1 promotes colorectal cancer cell migration and invasion by modulating miR-338-3p/TYRO3 axis. Cancer Cell Int 20:430
Guo J, Cheng Y (2018) MicroRNA-1247 inhibits lipopolysaccharides-induced acute pneumonia in A549 cells via targeting CC chemokine ligand 16. Biomed Pharmacother 104:60–68
Guo R, Zhang L, Meng J (2020) Circular RNA ANKRD36 attends to lipopolysaccharide-aroused MRC-5 cell injury via regulating microRNA-31-3p. BioFactors 46:391–401
Kulcheski FR, Christoff AP, Margis R (2016) Circular RNAs are miRNA sponges and can be used as a new class of biomarker. J Biotechnol 238:42–51
Kumar KJ, Ashok Chowdary KV, Usha HC, Kulkarni M, Manjunath VG (2018) Etiology of community acquired pneumonia among children in India with special reference to atypical pathogens. Lung India 35:116–120
le Roux DM, Zar HJ (2017) Community-acquired pneumonia in children—a changing spectrum of disease. Pediatr Radiol 47:1392–1398
Li W, An X, Fu M, Li C (2016) Emergency treatment and nursing of children with severe pneumonia complicated by heart failure and respiratory failure: 10 case reports. Exp Ther Med 12:2145–2149
Liang ZZ, Guo C, Zou MM, Meng P, Zhang TT (2020) circRNA-miRNA-mRNA regulatory network in human lung cancer: an update. Cancer Cell Int 20:173
Liu M, Han T, Shi S, Chen E (2018) Long noncoding RNA HAGLROS regulates cell apoptosis and autophagy in lipopolysaccharides-induced WI-38cells via modulating miR-100/NF-kappaB axis. Biochem Biophys Res Commun 500:589–596
Liu X, Zhao P, Ge W (2019) Knockdown of circular RNA circZNF652 remits LPS-induced inflammatory damage by regulating miR-181a. BioFactors. https://doi.org/10.1002/biof.1606
Liu G, Wan Q, Li J, Hu X, Gu X, Xu S (2020) Circ_0038467 regulates lipopolysaccharide-induced inflammatory injury in human bronchial epithelial cells through sponging miR-338-3p. Thorac Cancer 11:1297–1308
Ning G, Wang X, Wu D, Yin Z, Li Y, Wang H, Yang W (2017) The etiology of community-acquired pneumonia among children under 5 years of age in mainland China, 2001–2015: a systematic review. Hum Vaccin Immunother 13:2742–2750
Pu J, Wang J, Li W, Lu Y, Wu X, Long X, Luo C, Wei H (2020) hsa_circ_0000092 promotes hepatocellular carcinoma progression through up-regulating HN1 expression by binding to microRNA-338-3p. J Cell Mol Med. https://doi.org/10.1111/jcmm.15010
Qian W, Huang T, Feng W (2020) Circular RNA HIPK3 promotes EMT of cervical cancer through sponging miR-338-3p to up-regulate HIF-1alpha. Cancer Manag Res 12:177–187
Stadler JAM, Andronikou S, Zar HJ (2017) Lung ultrasound for the diagnosis of community-acquired pneumonia in children. Pediatr Radiol 47:1412–1419
Sun J, Wang X, Wang D, Zhao Z, Zhang L, Zhang J (2020) circAtp9b knockdown alleviates LPS-caused inflammation provided that microRNA-27a is upregulated. Int Immunopharmacol 78:105925
Wang L, Peng X, Lu X, Wei Q, Chen M, Liu L (2019) Inhibition of hsa_circ_0001313 (circCCDC66) induction enhances the radio-sensitivity of colon cancer cells via tumor suppressor miR-338-3p: effects of cicr_0001313 on colon cancer radio-sensitivity. Pathol Res Pract 215:689–696
Zhang Y, Zhu Y, Gao G, Zhou Z (2019) Knockdown XIST alleviates LPS-induced WI-38 cell apoptosis and inflammation injury via targeting miR-370-3p/TLR4 in acute pneumonia. Cell Biochem Funct 37:348–358
Zhang L, Dong L, Tang Y, Li M, Zhang M (2020) MiR-146b protects against the inflammation injury in pediatric pneumonia through MyD88/NF-kappaB signaling pathway. Infect Dis (Lond) 52:23–32
Zhao T, Zheng Y, Hao D, Jin X, Luo Q, Guo Y, Li D, Xi W, Xu Y, Chen Y, Gao Z, Zhang Y (2019) Blood circRNAs as biomarkers for the diagnosis of community-acquired pneumonia. J Cell Biochem 120:16483–16494
Zhou Z, Zhu Y, Gao G, Zhang Y (2019) Long noncoding RNA SNHG16 targets miR-146a-5p/CCL5 to regulate LPS-induced WI-38 cell apoptosis and inflammation in acute pneumonia. Life Sci 228:189–197
Funding
None.
Author information
Authors and Affiliations
Contributions
JW was responsible for drafting the manuscript. JW and GL contributed to the analysis and interpretation of the data. SL and LC contributed in the data collection. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no financial conflict of interest.
Ethical Approval
All procedures performed in studies involving animals were in accordance with the ethical standards of Fujian Maternity and Child Health Hospital.
Consent to Participate
N/A.
Consent for Publication
Written informed consent for publication was obtained from all participants.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Wang, J., Li, G., Lin, S. et al. Circ-BICC1 Knockdown Alleviates Lipopolysaccharide (LPS)-Induced WI-38 Cell Injury Through miR-338-3p/MYD88 Axis. Biochem Genet 61, 170–186 (2023). https://doi.org/10.1007/s10528-022-10242-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10528-022-10242-3