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Human Adipose-Derived Stem Cells Delay Muscular Atrophy after Peripheral Nerve Injury in Rats

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Abstract

Introduction

Given that denervation atrophy often occurs in muscle after peripheral nerve injury, the effects of injections of human adipose-derived stem cells (hADSCs) and platelet-rich plasma (PRP) into muscle after peripheral nerve injury were examined.

Methods

hADSCs were isolated from human subcutaneous fat tissue, and PRP was prepared from rat whole blood before injection into a rat sciatic nerve injury model. Muscle atrophy was evaluated by quantitating the gross musculature and muscle fiber area and walking footprint analysis.

Results

At 4 weeks post-surgery, there were significant differences in the sciatic functional index between experimental (injected with hADSCs, PRP, or combined hADSCs + PRP) and non-operated groups (p < 0.0001), but no significant differences were observed between the different treatment groups (p > 0.05). Post hoc Bonferroni tests also showed significant differences in the wet muscle weight ratios of hADSC, PRP, and combined groups compared to PBS group. The gastrocnemius muscle fiber area was larger in hADSC group and the combined group compared to PBS group at 4 weeks post-surgery.

Conclusion

The injection of hADSCs delays muscular atrophy after sciatic nerve injury in rats; thus, hADSCs are a promising alternative for regenerating atrophied muscle.

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Data Availability

All data generated or analyzed during this study are included in this published article and its supplementary information files.

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Funding

This study was supported by the Discipline Construction Project of Peking Union Medical College (201920200401) and CAMS Innovation Fund for Medical Sciences (2021-I2M-1-068).

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Authors and Affiliations

  1. 2nd Department, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 33 Badachu Road, Shijingshan District, Beijing, 100144, China

    Siwei Qu, Ning Ma, Weixin Wang, Sen Chen, Qi Wu, Yangqun Li & Zhe Yang

Authors
  1. Siwei Qu
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  2. Ning Ma
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  3. Weixin Wang
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  4. Sen Chen
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  5. Qi Wu
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  6. Yangqun Li
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  7. Zhe Yang
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Contributions

Q.S. performed the experiments, analyzed the data, and wrote the manuscript. M.N., W.W., C.S., W.Q., and L.Y. designed the research, analyzed the data, and contributed to the writing of the manuscript. Y.Z. supervised the study.

Corresponding author

Correspondence to Zhe Yang.

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Conflict of Interest

The authors declare no competing interests.

Ethical approval and consent to participate

All experiments conducted in this study were reviewed and approved by the Local Animal Ethics Committee (No. 202003003). Using human adipose tissue was approved by the Ethics Committee of Plastic Surgery Hospital (No. 2150019022). All methods in this study were conducted following relevant guidelines and regulations. All methods are reported in this study following ARRIVE guidelines for the reporting of animal experiments.

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Qu, S., Ma, N., Wang, W. et al. Human Adipose-Derived Stem Cells Delay Muscular Atrophy after Peripheral Nerve Injury in Rats. Cell Biochem Biophys 80, 555–562 (2022). https://doi.org/10.1007/s12013-022-01082-4

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  • DOI: https://doi.org/10.1007/s12013-022-01082-4

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