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Profiling the total single-cell transciptome using droplet microfluidics

Nature Biotechnology volume 40, pages 1766–1767 (2022)Cite this article

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Current high-throughput single-cell methods detect only a small part of the transcriptome. The workflow presented here integrates molecular analysis and droplet microfluidics to derive total transcriptomic atlases that encompass alternative splicing and non-coding transcripts in large numbers of single cells. The utility of this method is demonstrated by analysis of mouse gastrulation and early organogenesis.

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Fig. 1: Vast transcriptome analysis of single cells by dA-tailing (VASA-seq) captures the total transcriptome at scale and uncovers new cell-differentiation trajectories.

References

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This is a summary of: Salmen, F. et al. High-throughput total RNA sequencing in single cells using VASA-seq. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01361-8 (2022).

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Profiling the total single-cell transciptome using droplet microfluidics. Nat Biotechnol 40, 1766–1767 (2022). https://doi.org/10.1038/s41587-022-01370-7

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