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SLAH1 is involved in the long-distance transport of Cl from roots into shoots in the Cl-tolerant xerophyte Pugionium cornutum under salt stress

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Abstract

Purpose

Different from most crops and forages, the xerophyte Pugionium cornutum can absorb high quantities of Cl and then efficiently transport them into shoots to enhance osmotic adjustment ability under salt stress. However, the molecular mechanism underlying long-distance Cl transport remains unclear. SLAH1 is crucial for root-to-shoot Cl transport in Arabidopsis only under non-saline conditions. This study aimed to evaluate the function of PcSLAH1 from P. cornutum in long-distance Cl transport under salt stress.

Methods

PcSLAH1 was cloned, its tissue and subcellular locations and expression patterns in response to salt treatments were analyzed. The effect of PcSLAH1 on ion accumulation and the expression of key genes involved in Na+ and Cl transport were investigated by ectopically overexpressing in Arabidopsis driven by a root stelar-specific promoter.

Results

PcSLAH1 was specifically expressed at root stelar cells and located on the plasma membrane. Opposing with AtSLAH1 in Arabidopsis, the relative expression level of PcSLAH1 in roots was significantly induced by high Cl treatments. The root stelar-specific expression of PcSLAH1 in Arabidopsis resulted in a significant increase in shoot Cl content under NaCl or KCl treatment. PcSLAH1 could coordinate with CLCg and NHX1 to regulate shoot Cl and Na+ homeostasis under salt treatment. In addition, the root stelar-specific expression of PcSLAH1 conferred a stronger long-distance transport ability of Cl than AtSLAH1.

Conclusions

PcSLAH1 functions in facilitating the long-distance transport of Cl and modulating shoot Na+ and Cl homeostasis under salt stress. The up-regulated expression of PcSLAH1 is conducive to salt tolerance of P. cornutum.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We thank Dr. Bo Li from School of Life Science, Lanzhou University for the kind provision of Arabidopsis atslah1 mutant.

Funding

This work was financially supported by the National Natural Science Foundation of China (Grant Nos. 32171677 and 31730093), and Scientific Startup Foundation for Doctors of Northwest A and F University (Grant No. Z1090121101).

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Authors

Contributions

YNC and QM conceptualized and designed the study. YNC, XYL, RWL generated the data. YNC, XYL, RWL and ZHH analyzed the data. YNC, SMW and QM wrote the paper.

Corresponding author

Correspondence to Qing Ma.

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Cui, YN., Li, XY., Liu, RW. et al. SLAH1 is involved in the long-distance transport of Cl from roots into shoots in the Cl-tolerant xerophyte Pugionium cornutum under salt stress. Plant Soil 479, 631–648 (2022). https://doi.org/10.1007/s11104-022-05551-w

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  • DOI: https://doi.org/10.1007/s11104-022-05551-w

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