All-printed point-of-care immunosensing biochip for one drop blood diagnostics

Jimei Chi; Yuanbin Wu; Feifei Qin; Meng Su; Nan Cheng; Jiabing Zhang; Chunbao Li; Zewei Lian; Xu Yang; Lijun Cheng; Hongfei Xie; Huadong Wang; Zeying Zhang; Jan Carmeliet; Yanlin Song

doi:10.1039/D2LC00385F

All-printed point-of-care immunosensing biochip for one drop blood diagnostics†

Jimei Chi,^ab Yuanbin Wu,^c Feifei Qin,

^d Meng Su,*^ab Nan Cheng,*^c Jiabing Zhang,^e Chunbao Li,^f Zewei Lian,^ab Xu Yang,^ab Lijun Cheng,^ab Hongfei Xie,^ab Huadong Wang,^ab Zeying Zhang,^ab Jan Carmeliet^d and Yanlin Song

*^ab

Author affiliations

* Corresponding authors

^a Key Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Beijing Engineering Research Center of Nanomaterials for Green Printing Technology, Beijing National Laboratory for Molecular Sciences (BNLMS), Institute of Chemistry, Chinese Academy of Sciences (ICCAS), Beijing 100190, P. R. China
E-mail: sumeng1988@iccas.ac.cn, ylsong@iccas.ac.cn

^b University of Chinese Academy of Sciences, Beijing 100049, P. R. China

^c Department of Cardiovascular Surgery, PLA General Hospital, Beijing 100853, P. R. China
E-mail: cn86919@163.com

^d Department of Mechanical and Process Engineering, Swiss Federal Institute of Technology in Zürich (ETH Zürich), Zürich 8093, Switzerland

^e Graduate School of Medical School of Chinese PLA Hospital, Beijing, P. R. China

^f Department of Orthopaedic Medicine, Fourth Medical Center, PLA General Hospital, Beijing 100853, China

Abstract

Designing and preparing a fast and easy-to-use immunosensing biochip are of great significance for clinical diagnosis and biomedical research. In particular, sensitive, specific, and early detection of biomarkers in trace samples promotes the application of point-of-care testing (POCT). Here, we demonstrate an all-printed immunosensing biochip with the characteristics of hydrodynamic enrichment and photonic crystal-enhanced fluorescence. Direct quantitative detection of cardiac biomarkers via one drop of blood is achieved in 10 min. After simulating the hydrodynamic behavior of one droplet serum on the printed assay, creatine kinase-MB (CK-MB) has been recognized and located on the photonic crystal arrays. Benefiting from the fluorescence enhancement effect, quantitative detection of CK-MB has been demonstrated from 0.01 ng ml⁻¹ to 100 ng ml⁻¹, which is superior to the conventional enzyme-linked immunosorbent assay (ELISA). This strategy provides a general and easy-to-use approach for fast quantitative detection of biomarkers, which would be improved further for portable clinical diagnostics and home medical monitoring.

This article is part of the themed collection: Miniaturised Sensors & Diagnostics

Lab on a Chip

All-printed point-of-care immunosensing biochip for one drop blood diagnostics†

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All-printed point-of-care immunosensing biochip for one drop blood diagnostics

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