Abstract
Heart failure (HF) is a leading cause of death that has remained incurable. Recently, stem cell therapy has emerged as a promising tool in cardiac regenerative medicine. Human amniotic membrane-derived mesenchymal stem cells (hAMSCs) with unique characteristics can be used in HF treatment. Here, we aimed to examine the effects of hAMSCs transplantation on cardiac fibrosis in a rat model of ISO-induced HF. Forty male Wistar rats were divided into four groups: sham, isoproterenol-induced HF ((Iso)-)ISO, ISO + culture medium, and ISO + hAMSCs. HF was induced by subcutaneous injection of isoproterenol 170 mg/kg/d in 4 consecutive days. Four weeks later, in ISO + hAMSCs, 3 × 106 hAMSCs were injected into the myocardium, whereas the ISO + culture medium was only injected by cell culture medium. Finally, cardiac functions and hemodynamic parameters were measured. Immunohistochemistry (IHC), Western blot, and histological assessment were performed to evaluate myocardial fibrosis and detect vascular endothelial growth factor (VEGF) collagen type I and III expression level. HF model caused a decrease in ejection fraction (EF) and fraction shortening, whereas both were increased after hAMSCs transplantation. IHC and Western blot and Western blot analyses confirmed that hAMSCs could attenuate fibrosis, reduce collagen I and III depositions, and increase VEGF expression. Intramyocardial transplantation of hAMSCs improves cardiac functions and myocardial structure caused by HF. A rise in VEGF expression presents hAMSCs as a compatible source of stem cell therapy for HF.
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Present work was funded by a research grant from Physiology Research Center of Iran University of Medical Sciences.
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NA was involved in conceptualization and study design; SMTRT and MN contributed to investigation and data collection; YA was involved in writing the original draft; NA, SMTRT, YA and MN contributed to writing—review and editing.
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The animal use, experimental procedures, and care protocols were approved by School of Medicine Animal Care and Use Committee of Iran University of Medical Sciences. All protocols and animal operation rules were confirmed by the Animal Ethical Committee of Iran University of Medical Sciences.
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The amniotic membranes were provided from Shahid Akbar Abadi Hospital upon informed consent of the participants in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
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Razavi Tousi, S.M.T., Sharifi, M., Naseroleslami, M. et al. Mesenchymal Stem Cells Derived from Human Amniotic Membrane Increase VEGF and Extenuate Fibrosis in Heart Failure Rats. Iran J Sci Technol Trans Sci 46, 781–791 (2022). https://doi.org/10.1007/s40995-022-01307-4
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DOI: https://doi.org/10.1007/s40995-022-01307-4