Abstract
Aims
To evaluate the occurrence, the characterization and identity of nodulating bacteria in symbiosis with Mimosa spp. in the ultramafic massif of Barro Alto, Goiás state, Brazil.
Methods
Nodules from field grown M. somnians and M. claussenii were sampled for bacteria isolation and in situ detection using microscopy. Isolates were characterized for their nodulation capacity on M. pudica and common bean, and their tolerance to Ni in culture medium. Bacteria were also partially identified by their 16S rRNA gene sequences. In addition, recA, gyrB, nodC and nifH genes from five representative isolates were sequenced for phylogenetic studies.
Results
In situ detection indicated the exclusive presence of Paraburkholderia sp. within the nodules. This identification was confirmed for most of the isolates by the analysis of their 16S rRNA gene sequences. All isolates identified as Paraburkholderia sp. were able to effectively nodulate M. pudica, but those tested in common bean produced ineffective nodules. Inoculation tests showed that these bacteria improved M. pudica growth in the absence of nitrogen. None of the isolates showed Ni tolerance. The concatenated 16S rRNA, recA and gyrB, and the nodC and nifH genes sequences demonstrated that the five selected isolates were closely related to P. atlantica.
Conclusions
This is the first report of Paraburkholderia present in nodules from Mimosa plants naturally growing in ultramafic soils. These results suggest that this symbiosis may be a factor to be considered as part of programs to restore ultramafic soils in Barro Alto areas degraded by mining activity.
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Acknowledgements
This work was supported by the Brazilian Agricultural Research Corporation (Embrapa, projects 02.13.08.001.00.00; 03.14.00.026.00.00; 03.14.00.094.00.00), the National Council for Scientific and Technological Development (CNPq) Edital Universal (grant number 485196/2012-3), the MCTI/CNPq/CAPES/FAPS (INCT-MPCPAgro), and by the Mineradora Anglo American do Brasil. Clemente Batista Soares Neto was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES) - finance code 001. Paulo Ivan Fernandes-Júnior; Jerri Edson Zilli, and Ieda de Carvalho Mendes are research fellows of CNPq.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Clemente Batista Soares Neto, Paula Rose de Almeida Ribeiro, Jerri Edson Zilli, Euan Kevin James, and Fábio Bueno dos Reis Junior. The first draft of the manuscript was written by Clemente Batista Soares Neto, Paulo Ivan Fernandes-Júnior, Helson Mario Martins do Vale and Fábio Bueno dos Reis Junior and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Soares Neto, C.B., Ribeiro, P.R.A., Fernandes-Júnior, P.I. et al. Paraburkholderia atlantica is the main rhizobial symbiont of Mimosa spp. in ultramafic soils in the Brazilian Cerrado biome. Plant Soil 479, 465–479 (2022). https://doi.org/10.1007/s11104-022-05536-9
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DOI: https://doi.org/10.1007/s11104-022-05536-9