Abstract
In the present study, the lipase from Aspergillus fumigatus was purified which was found to be stable to commercial detergents and oxidising agents. A purification fold of 6.96 and yield of 11.03% were achieved when the enzyme was purified using Octyl Sepharose column chromatography. In presence of various oxidizing agents, the highest activity of lipase was 15.56 U/mg with hydrogen peroxide. Among various surfactants used, the maximum activity exhibited by lipase was with Tween 80. While studying the effect of various detergents, the highest activity of 9.3 U/mg was achieved with "Vanish" detergent. Wash performance was studied with various detergents out of which "Vanish" showed highest oil removal of 79%. Lipase from Aspergillus fumigatus possessed better stability with various surfactants and oxidizing agents. The results of this study have shown that the lipase from Aspergillus fumigatus along with detergent "Vanish" (0.7%) under optimized conditions (5 μg/ml lipase, 40°C wash temperature and 40 min wash duration) improved oil removal from cotton fabric stained with mustard oil by 84%.
Zusammenfassung
In der vorliegenden Studie wurde die Lipase aus Aspergillus fumigatus gereinigt, die sich gegenüber handelsüblichen Waschmitteln und Oxidationsmitteln als stabil erwies. Eine Reinigungsfalte von 6,96 und eine Ausbeute von 11,03% wurden erreicht, als das Enzym mit Octyl-Sepharose-Säulenchromatographie gereinigt wurde. In Anwesenheit verschiedener Oxidationsmittel betrug die höchste Aktivität der Lipase 15,56 U/mg mit Wasserstoffperoxid. Unter den verschiedenen verwendeten Tensiden war die maximale Aktivität der Lipase mit Tween 80 zu verzeichnen. Bei der Untersuchung der Wirkung verschiedener Waschmittel wurde die höchste Aktivität von 9,3 U/mg mit dem Waschmittel Vanish erreicht. Die Waschleistung wurde mit verschiedenen Waschmitteln untersucht, von denen "Vanish" die beste Ölentfernung von 79% zeigte. Lipase aus Aspergillus fumigatus besaß eine bessere Stabilität mit verschiedenen Tensiden und Oxidationsmitteln. Die Ergebnisse dieser Studie haben gezeigt, dass die Lipase aus Aspergillus fumigatus zusammen mit dem Waschmittel ,,Vanish" (0,7%) unter optimierten Bedingungen (5 μg/ml Lipase, 40°C Waschtemperatur und 40 min Waschdauer) die Ölentfernung aus mit Senföl angeschmutzem Baumwollstoff um 84% verbesserte.
Funding statement: The financial support from DEST (Department of Environment, Science and Technology), Himachal Pradesh to Ms. Akshita Mehta in the form of Project Fellow is thankfully acknowledged. The Fellowship granted to Mr. Kamal Kumar Bhardwaj in the form of SRF from the Council of Scientific & Industrial Research under Ministry of Human Resource Development, Government of India is thankfully acknowledged.
About the authors
Mrs. Akshita Mehta is currently pursuing her doctoral research from Department of Biotechnology, Himachal Pradesh University, Shimla, HP, India. She has received her Master’s degree in Biotechnology from Shoolini Institute of Life Science and Business Management, Solan, HP, India and Bachelors in Industrial Microbiology from M.L.N. College Yamunanagar, Haryana, India. Her research interest is mainly in enzyme technology and applications of lipase in various fields.
Ms. Aashna Gupta is currently working as assistant editor at Taylor and Francis in clinical research. She has received her Master’s degree in Microbiology from Himachal Pradesh University, Shimla, HP, India and Bachelors in Life Sciences from Delhi University (Acharya Narendra Dev College). Her research interest is mainly in enzymes and their applications in microbiology field.
Mr. Kamal Kumar Bhardwaj is currently pursuing his doctoral research from Department of Biotechnology, Himachal Pradesh University, Shimla, HP, India. He has received his Master’s degree in Biotechnology from Shoolini Institute of Life Science and Business Management, Solan, HP, India and Bachelors in Medical from P.G. College Hamirpur, India. His research interest is mainly in enzyme technology and application of esterase in plastic degradation.
Prof. Reena Gupta is presently working as Professor in the Department of Biotechnology, Himachal Pradesh University, Summerhill, Shimla. Her area of specialization is Biochemistry, Immunology and Enzyme Technology. She received her Ph.D. from Panjab University, Chandigarh and Post Doctorate from IMTECH (Institute of Microbial Technology), Chandigarh, India. She has 24 years of teaching and 32 years of research experience with four completed research projects. She has guided 9 Ph. D. students and 26 M.Phil. students. She has 106 publications in international and national journals.
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Author’s Contributions:
Mrs. Akshita Mehta had performed the experimental work and result analysis.
Ms. Aashna Gupta assisted in the methodology and as well as in experiments done.
Mr. Kamal Kumar Bhardwaj helped in the writing of the manuscript.
Dr. Reena Gupta had designed the experiments and critically revised writing and the organization of the manuscript.
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Conflicts of interest:
The author(s) declare(s) that there is no conflict of interests regarding the publication of this article.
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