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Preparation of Chitosan with High Antibacterial Efficiency from Penicillium crustosum TZ18

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Abstract

Fungi are accepted as novel sources of chitinous materials. The present study was performed to produce chitinous material from locally isolated Penicillium crustosum TZ18 (GenBank accesion number: OM095448) using molasses as substrate, prepare chitosan from chitinous material and investigate the antibacterial activity of the prepared chitosan. An initial pH of 6.0, a molasses concentration of 120 g/L, a temperature of 25 °C and an incubation time of 5 days were the most favourable parameters for the production of chitinous material. Under the optimized culture conditions, fungal biomass of 29.5 g/L and total chitinous material of 7.79 g/L (26.4%) could be produced. The chitin and chitosan concents of chitinous material were 97 (7.56 g/L) and 3% (0.23 g/L), respectively. Chitin fraction was converted into the chitosan (Penicillium chitosan, PC) by deacetylation process. FT-IR results revealed that the functional groups of PC and commercial chitosan (CC) were very similar to each other. Deacetylation degrees of PC and CC were found to be 78.3 and 76.9%, respectively. The viscosity-average molecular weights of PC and CC were determined as 122.7 and 298.3 kDa, respectively. When compared to CC, PC was found to have higher antibacterial efficiency against both Escherichia coli and Staphylococcus aureus. High antibacterial efficiency of the chitosan could be ascribed to its low molecular weight and high deacetylation degree. The chitosan may be useful for biomadical applications. The production of chitinous material from P. crustosum and the antibacterial efficiency of P. crustosum chitosan were investigated for the first time in the present study.

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The data and materials that support the findings of this study are openly available on request.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Science Faculty, Ataturk University, Erzurum, Turkey

    Fatih Kayar, Hakan Ozkan, Ahmet Adiguzel & Mesut Taskin

  2. Department of Medical Services and Techniques, Hinis Vocational College, Ataturk University, Erzurum, Turkey

    Meryem Doymus

  3. Vocational School of Health Services, Bingol University, Bingol, Turkey

    Nazli Pinar Arslan

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  1. Fatih Kayar
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  2. Meryem Doymus
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  3. Hakan Ozkan
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Contributions

FK: Methodology, conceptualization, data analysis and investigation. MD: Methodology, investigation and data analysis. HO: Methodology, investigation and writing-review & editing. NPA: Methodology and investigation. AA: Investigation and formal analysis. MT: Investigation, writing—review & editing, supervision.

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Kayar, F., Doymus, M., Ozkan, H. et al. Preparation of Chitosan with High Antibacterial Efficiency from Penicillium crustosum TZ18. J Polym Environ 30, 3598–3608 (2022). https://doi.org/10.1007/s10924-022-02458-6

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