Semi-automated preparation of fine-needle aspiration samples for rapid on-site evaluation

Filipe Marques; Janosch Hauser; Emre Iseri; Igor Schliemann; Wouter van der Wijngaart; Niclas Roxhed

doi:10.1039/D2LC00241H

Semi-automated preparation of fine-needle aspiration samples for rapid on-site evaluation†

Filipe Marques,

^a Janosch Hauser,

^a Emre Iseri,

^a Igor Schliemann,^b Wouter van der Wijngaart^a and Niclas Roxhed*^ac

Author affiliations

* Corresponding authors

^a Division of Micro and Nanosystems, KTH Royal Institute of Technology, 10044 Stockholm, Sweden
E-mail: roxhed@kth.se

^b Pathology and Cytology Department, Karolinska University Hospital, Stockholm, Sweden

^c Medtech Labs, Karolinska hospital, Stockholm, Sweden

Abstract

Rapid on-site evaluation (ROSE) significantly improves the diagnostic yield of fine needle aspiration (FNA) samples but critically depends on the skills and availability of cytopathologists. Here, we introduce a portable device for semi-automated sample preparation for ROSE. In a single platform, the device combines a smearing tool and a capillary-driven chamber for staining FNA samples. Using a human pancreatic cancer cell line (PANC-1) and liver, lymph node, and thyroid FNA model samples, we demonstrate the capability of the device to prepare samples for ROSE. By minimizing the equipment needed in the operating room, the device may simplify the performance of FNA sample preparation and lead to a wider implementation of ROSE.

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Article information

DOI: https://doi.org/10.1039/D2LC00241H
Article type: Paper
Submitted: 14 Mar 2022
Accepted: 06 May 2022
First published: 06 May 2022
This article is Open Access

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Lab Chip, 2022,22, 2192-2199

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Semi-automated preparation of fine-needle aspiration samples for rapid on-site evaluation

F. Marques, J. Hauser, E. Iseri, I. Schliemann, W. van der Wijngaart and N. Roxhed, Lab Chip, 2022, 22, 2192 DOI: 10.1039/D2LC00241H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Lab on a Chip

Semi-automated preparation of fine-needle aspiration samples for rapid on-site evaluation†

Abstract

Supplementary files

Article information

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Semi-automated preparation of fine-needle aspiration samples for rapid on-site evaluation

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