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Combining high tolerance to drought with high tolerance to salinity in Egyptian wheat (Triticum aestivum L.) cultivars

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Abstract

Eleven wheat cultivars were obtained for this study, and three treatments were carried out: salinity treatment in a greenhouse, drought treatment in hydroponics and drought with salinity treatment in hydroponics. In the case of salinity treatment in a greenhouse, the cultivars Sids1 and Giza168 showed a high tolerance to salinity. In the drought treatment in hydroponics using PEG6000 in water irrigation, Sids1 and Giza168 showed a high tolerance to drought. In the drought with salinity treatment in hydroponics using PEG6000 with NaCl 8000 ppm in water irrigation, the cultivars Sahel1 and Misr2 showed medium tolerance to salinity and drought. Also, phenotypic parameters of the cultivars were measured. The tallest cultivar was Misr 1 99.67 cm, while Sids1 showed the highest thousand grain weight at 46.81 g. Based on these results, we selected five cultivars of wheat: Sahel1, Gemmeiza10, Misr1, Sakha93 and Giza168 to complete this study and were done carried out a complete diallele hybridization and reverse hybridization between group one: Sahel1 and Gemmeiza10, group two: Misr1 and Sakha93 and group three: Giza168 and Gemmeiza10. The transcripts levels of salinity (Na+/H+ antiporter) and drought (DREB2) genes in the three groups of wheat hybrid were determined using qRT-PCR; DREB2 gene transcripts significantly in group one by 0.79-fold were compared to group three mRNA levels of DREB2 gene that was 0.24-fold. The change in mRNA levels of DREB2 gene of group two was by 0.2-fold, while the mRNA transcript levels of salinity (Na+/H+ antiporter) gene raised no significantly in the groups. In group one in this group the fold change was 0.0037-fold, while mRNA levels of Na+/H+ antiporter gene was 0.0041-fold, in group two. In group three was found the fold change 0.0024-fold. The (DREB2 & Na+/H+ antiporter) genes in hybrid wheat were identified by amplification followed by sequencing. Our results suggest that the best Egyptian wheat cultivars can be grown under difficult environmental conditions in Egypt.

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This work was accomplishment by self-funding from the authors.

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Authors and Affiliations

  1. Department of Genetics, Faculty of Agriculture, Benha University, Qalubia, Egypt

    Islam Mahmoud Ahmed & Mohamed AbdelHamid Gomaa

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  1. Islam Mahmoud Ahmed
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  2. Mohamed AbdelHamid Gomaa
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The authors cooperated in all steps of this study. All of the authors read and approved the final manuscript.

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Correspondence to Islam Mahmoud Ahmed or Mohamed AbdelHamid Gomaa.

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Communicated by A. M. Alqudah and M. Taylor.

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Ahmed, I.M., Gomaa, M.A. Combining high tolerance to drought with high tolerance to salinity in Egyptian wheat (Triticum aestivum L.) cultivars. CEREAL RESEARCH COMMUNICATIONS 50, 717–732 (2022). https://doi.org/10.1007/s42976-022-00264-3

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