Abstract
The paper describes the synthesis and characterization of pure-silica BEA zeolite and silicalite-1 (Si–MFI) samples that were used for the adsorption immobilization of Aspergillus oryzae β-galactosidase. The highest adsorption capacity (17 mg/g) and the highest catalytic activity (32% of the enzyme activity in solution) were achieved on the BEA sample. Despite the equality in the enzyme catalytic activity on both samples of silicalite-1 (specifically, 27% of the enzyme activity in solution), the enzyme adsorption capacity on the fine-crystalline sample (14 mg/g) proved to be several-fold higher than that on the coarse-crystalline sample (3.6 mg/g).
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Funding
The synthesis and physicochemical examination of the samples were performed with financial support from the Russian Science Foundation (RSF Grant no. 20-13-00203). The adsorption and catalytic properties of the enzyme were investigated within the State Program “Physical chemistry of surface, adsorption and catalysis.”
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Atyaksheva, L.F., Dobryakova, I.V., Kostyukov, I.A. et al. Adsorption of Lactase on Pure-Silica BEA Zeolite and Silicalite-1. Pet. Chem. 62, 316–321 (2022). https://doi.org/10.1134/S0965544122030021
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DOI: https://doi.org/10.1134/S0965544122030021