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Calcium, Ca2+-ATPase, Calmodulin, and Calbindin D-28KD Localization in Testis of Leptodactylus chaquensis (Anura: Leptodactylidae)

Part of: Micrographia Collection

Published online by Cambridge University Press:  17 March 2022

Lucrecia Iruzubieta-Villagra Open the ORCID record for Lucrecia Iruzubieta-Villagra [Opens in a new window] ,
Inés Ramos ,
Susana Cisint ,
Claudia A. Crespo  and
Silvia Fernández
Lucrecia Iruzubieta-Villagra*
Affiliation:
Facultad de Bioquímica, Química y Farmacia, Instituto de Biología, Universidad Nacional de Tucumán, Chacabuco 461, 4000 S.M. de Tucumán, Argentina
Inés Ramos
Affiliation:
Facultad de Bioquímica, Química y Farmacia, Instituto de Biología, Universidad Nacional de Tucumán, Chacabuco 461, 4000 S.M. de Tucumán, Argentina Instituto Superior de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas — Universidad Nacional de Tucumán, Chacabuco 461, 4000 S.M. de Tucumán, Argentina
Susana Cisint
Affiliation:
Facultad de Bioquímica, Química y Farmacia, Instituto de Biología, Universidad Nacional de Tucumán, Chacabuco 461, 4000 S.M. de Tucumán, Argentina
Claudia A. Crespo
Affiliation:
Facultad de Bioquímica, Química y Farmacia, Instituto de Biología, Universidad Nacional de Tucumán, Chacabuco 461, 4000 S.M. de Tucumán, Argentina Instituto Superior de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas — Universidad Nacional de Tucumán, Chacabuco 461, 4000 S.M. de Tucumán, Argentina
Silvia Fernández
Affiliation:
Facultad de Bioquímica, Química y Farmacia, Instituto de Biología, Universidad Nacional de Tucumán, Chacabuco 461, 4000 S.M. de Tucumán, Argentina Instituto Superior de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas — Universidad Nacional de Tucumán, Chacabuco 461, 4000 S.M. de Tucumán, Argentina
*
*Corresponding author: Lucrecia Iruzubieta-Villagra, E-mail: ana.iruzubietavillagra@fbqf.unt.edu.ar
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Abstract

The intracellular localization of Ca2+, Ca2+-ATPase, Calmodulin, and Calbindin D-28KD have been studied in testes of the toad Leptodactylus chaquensis, using ultracytochemical and immunohistochemical techniques. The Ca2+ presences in the nucleus and into the mitochondria of the germ cells, together with the activity of Ca2+-ATPase detected in the nuclear envelope and mitochondrial crests, suggest the participation of this transporter in the storage of Ca2+. In Sertoli cells, Ca2+ deposits were also found in vesicles and lamellar bodies. Calmodulin and Calbindin D-28KD were revealed in the cytoplasm of both cell types. At the spermatozoon level, the cation deposits were located in the subacrosomal space and in the acrosomal vesicle. Ca2+-ATPase activity was observed in the acrosomal and plasma membranes of the gamete that suggests the existence of a transport system responsible for maintaining low cytoplasmic Ca2+ levels. The activity of Ca2+-ATPase and the location of Ca2+ deposits in gamete tail would be related to flagellar movement. The colocalization of Ca2+ deposits and their binding proteins in efferent duct cells would probably be associated with secretory activity. Considering that intracellular Ca2+ is present in different gonadal cells, this work would provide a better understanding of the cation importance in the testicular functions of this species.

Keywords

amphibianscalciumcalcium-binding proteinspyroantimonateTEM
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 28 , Issue 3 , June 2022 , pp. 869 - 879
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the Microscopy Society of America

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