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Ursane-type triterpene oligoglycosides with anti-hepatosteatosis and anti-hyperlipidemic activity from the leaves of Ilex paraguariensis A. St.-Hil.

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Abstract

The methanol extract from the leaves of Ilex paraguariensis A. St.-Hil. (Aquifoliaceae), popularly known as mate, maté, or yerba maté, inhibits the intracellular triglyceride accumulation in HepG2 cells and suppresses the plasma triglyceride elevation in olive oil-treated mice. Three new triterpene saponins, termed mateosides I (1), II (2), and III (3), were isolated from the extract along with 29 known compounds. The structures of 1–3 were elucidated based on chemical and spectroscopic evidence. Among the isolates, principal saponin constituents, 2 and matesaponins 1 (7) and 2 (9), potently inhibited the triglyceride accumulation in HepG2 cells simultaneously treated with oleic acid and high glucose. In vivo assay of the methanol extract of I. paraguariensis revealed that 7 and 9 showed anti-hyperlipidemic activities in olive oil-treated mice. These results suggested that the saponin constituents of I. paraguariensis could be valuable bioactive marker for the anti-obesogenic activity.

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Acknowledgements

The authors gratefully thank the Division of Joint Research Center, Kindai University, for the NMR and MS measurements. We would like to thank Editage (www.editage.com) for English language editing.

Funding

This work was supported by the JSPS KAKENHI, Japan [Grant Numbers 18K06726 (T.M.) and 18K06739 (K.N.)].

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  1. Kiyofumi Ninomiya

    Present address: School of Pharmacy, Shujitsu University, 1-6-1 Nishigawara, Naka-ku, Okayama, Okayama, 703-8516, Japan

Authors and Affiliations

  1. Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan

    Akifumi Nagatomo, Naoki Inoue, Takuya Konno, Yin Xu, Chinatsu Sakamoto, Mayuko Sone, Aya Shibasaka, Osamu Muraoka, Kiyofumi Ninomiya, Masayuki Yoshikawa, Yoshiaki Manse & Toshio Morikawa

  2. Antiaging Center, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan

    Osamu Muraoka, Kiyofumi Ninomiya & Toshio Morikawa

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  1. Akifumi Nagatomo
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Nagatomo, A., Inoue, N., Konno, T. et al. Ursane-type triterpene oligoglycosides with anti-hepatosteatosis and anti-hyperlipidemic activity from the leaves of Ilex paraguariensis A. St.-Hil.. J Nat Med 76, 654–669 (2022). https://doi.org/10.1007/s11418-022-01614-5

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