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The Cellular Behavior, Intracellular Signaling Profile and Nuclear-Targeted Potential Functions of Porcine Growth Hormone (pGH) in Swine Testicular Cells

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Abstract

Porcine growth hormone (pGH) has many important biological functions and roles, and the biological activity of pGH is closely related with its cell behavior and characteristics. However, so far, the behavior of pGH in swine testicular cell remains unclear. For this, in the current work, the swine testicular cell line (ST) was used as an in vitro model, and CLSM (Confocal laser scanning microscope), IFA (Indirect immunofluorescence assay), FCM (Flow cytometry) and WB (Western-blotting) were used to explore the pGH’s cell behivior and function, and the results showed that pGH and GHR could internalize into ST cell and transported to the nucleus. Furthermore, we studied the internalization kinetics of pGH and GHR on ST cell, and found that pGH and GHR internalizes into ST cell in a time-dependent manner. More importantly, we also investigated the potential molecular functions of pGH-GHR after it entered into the cell nuclei. The results indicated that nuclear-localized GHR could participate in cell proliferation by regulating the signal intensity of STAT5. In summary, our current research shows that the nuclear-localized pGH-GHR participates in the cell proliferation of ST cell, which lays a solid foundation for further research on the regulatory effect of pGH on testicular tissue.

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Acknowledgements

Y.Z. thanks S.L. (The Third Operating Room, Jilin University First Hospital, Changchun, China, 130118) and all colleagues for assistance with this study.

Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 30162022).

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Authors and Affiliations

  1. College of Animal Science and Technology, Jilin Agricultural University, Changchun, China, 130118

    Yan Zhang, Qingrong Zhao, Deyi Wu, Min Wu, Suo Li, Xin Zheng & Hainan Lan

  2. The Third Operating Room, Jilin University First Hospital, Changchun, China, 130118

    Shichun Li

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  1. Yan Zhang
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  2. Qingrong Zhao
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  7. Xin Zheng
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  8. Hainan Lan
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Contributions

Y.Z. and H.L. conceived and designed the experiments. Y.Z., Q.Z., and D.W. performed the experiments. Y.Z., M.W., S.L., and X.Z. analyzed the data. H.L. and S.L. (The Third Operating Room, Jilin University First Hospital, Changchun, China, 130118) contributed reagents/materials/analysis tools. Y.Z. and H.L. contributed to the writing of the manuscript.

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Correspondence to Hainan Lan.

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Zhang, Y., Zhao, Q., Wu, D. et al. The Cellular Behavior, Intracellular Signaling Profile and Nuclear-Targeted Potential Functions of Porcine Growth Hormone (pGH) in Swine Testicular Cells. Cell Biochem Biophys 80, 403–414 (2022). https://doi.org/10.1007/s12013-022-01068-2

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