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Description of Longidorus armeniacae n. sp. (Nematoda: Longidoridae), associated with Prunus armeniaca L. in Semnan province, Iran

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Abstract

Longidorus armeniacae n. sp. is described and illustrated using morphological and molecular data. It was recovered from the rhizosphere of apricot trees in Semnan province, northeast Iran. The new species is characterized by having females with plump (body width at vulva 80–123 μm, a = 44.5–68.2), medium sized body 5.0–7.4 mm long, lip region 16–20 μm wide, continuous with body contour, amphidial fovea small, pouch-like, guiding ring at 29–35 μm distance from the anterior end, odontostyle and odontophore 101–121 and 68–89 μm long, respectively, pharyngeal bulb 125–154 μm long, vulva at 48.8–59.3%, tail short (c′ = 0.5–0.7), rounded, sometimes varying to short conical; males common with 80–107 μm long spicules, 8–13 ventromedian supplements; four juvenile developmental stages present and tail in J1 with a digitate mucro. The morphological differences of the new species with 15 species having comparable morphology and/or close phylogenetic affinities namely L. arthensis, L. athesinus, L. caespiticola, L. carniolensis, L. cholevae, L. helveticus, L. iuglandis, L. lignosus, L. magnus, L. picenus, L. pius, L. poessneckensis, L. profundorum, L. raskii and L. uroshis are discussed. The polytomous identification codes of the new species are as follows: A45-B34-C23-D3-E1-F234-G1-H1-I2-J1-K7. The phylogenetic relationships of the new species and other selected species of the genus were reconstructed using LSU D2-D3 and the ITS rDNA sequences.

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Notes

  1. The specific epithet refers to the associated apricot plant, Prunus armeniaca L.

References

  • Archidona-Yuste, A., Navas-Cortés, J. A., Cantalapiedra-Navarrete, C., Palomares-Rius, J. E., & Castillo, P. (2016). Unravelling the biodiversity and molecular phylogeny of needle nematodes of the genus Longidorus (Nematoda: Longidoridae) in olive and a description of six new species. PLoS One, 11(1), e0147689.

    Article  Google Scholar 

  • Archidona-Yuste, A., Cantalapiedra-Navarrete, C., Castillo, P., & Palomares-Rius, J. E. (2019). Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain. Contributions to Zoology, 88, 1–41.

    Article  Google Scholar 

  • Altherr, E. (1974). Nematodes de la nappe phreatique du réseau fluvial de la Saale (Thuringe). II. Limnologica, 9, 81–132.

    Google Scholar 

  • Bakhshi Amrei, S., Peneva, V., Rakhshandehroo, F., & Pedram, M. (2020). Occurrence of Longidorus behshahrensis sp. n. (Nematoda: Longidoridae) in natural forests of Abbas Abad, northern Iran. European Journal of Plant Pathology, 156, 387–398.

    Article  CAS  Google Scholar 

  • Barsi, L., & De Luca, F. (2005). Morphological and molecular characterization of Longidorus helveticus (Nematoda, Dorylaimida) from Serbia. Nematologia Mediterranea, 33, 41–49.

    Google Scholar 

  • Barsi, L., & De Luca, F. (2008). Morphological and molecular characterization of Longidorus pius (Nematoda: Longidoridae) from the republic of Macedonia. Nematology, 10, 63–68.

    Article  CAS  Google Scholar 

  • Barsi L., & Lamberti, F. (2001). Longidorus pius sp. n. (Nematoda: Longidoridae) from Macedonia. Nematologia Mediterranea, 29, 207–213.

  • Brown, D. J. F., Grunder, J., Hooper, D. J., Klingler, J., & Kunz, P. (1994). Longidorus arthensis sp. n. (Nematoda: Longidoridae), a vector of cherry rosette disease caused by a new nepovirus in cherry trees in Switzerland. Nematologica, 40, 133–149. https://doi.org/10.1163/003525994X00094.

  • Cai, R., Archidona-Yuste, A., Cantalapiedra-Navarrete, C., Palomares-Rius, J. E., & Castillo, P. (2020). New evidence of cryptic speciation in the family Longidoridae (Nematoda: Dorylaimida). Journal of Zoological Systematics and Evolutionary Research, 58, 1–31.

    Article  Google Scholar 

  • Chen, Q., Hooper, D. J., Loof, P. A. A., & Xu, J. (1997). A revised polytomous key for the identification of species of the genus Longidorus Micoletzky, 1922 (Nematoda: Dorylaimoidea). Fundamental and Applied Nematology, 20, 15–28.

    Google Scholar 

  • Chizhov, V. N., Subbotin, S. A., Romanenko, N. D., & Kruchina, S. N. (1991). New nematode species of the genera Xiphinema and Longidorus (Nematoda: Longidoridae) from West Caucasus. Zoologichesky Zhurnal, 70, 130–133.

    Google Scholar 

  • Cobb, N. A. (1913). New nematode genera found inhabiting fresh water and non-brackish soils. Journal of the Washington Academy of Sciences, 3, 432–444.

    Article  Google Scholar 

  • Cohn, E. (1970). Observations on the feeding and symptomatology of Xiphinema and Longidorus on selected host roots. Journal of Nematology, 2, 167–173.

    CAS  PubMed  PubMed Central  Google Scholar 

  • De Grisse, A. T. (1969). Redescription ou modifications de quelques techniques utilisées dans l’étude des nematodes phytoparasitaires. Mededelingen Rijksfakulteit Landbouwwetenschappen, Gent, 34, 351–69.

  • Decraemer, W., & Chaves, E. (2012). Longidoridae and Trichodoridae. In R. H. Manzanilla-Lopez & N. Marbán-Mendoza (Eds.), Practical plant nematology (pp. 579–617). Montecillo.

    Google Scholar 

  • Gharibzadeh, F., Pourjam, E., & Pedram, M. (2018). Description of Longidorus azarbaijanensis n. sp. (Dorylaimida: Longidoridae) from Iran. Journal of Nematology, 50, 207–218.

    Article  Google Scholar 

  • Handoo, Z. A., Kantor, M. R., & Khan, E. (2021). Description of ten new species including two new genera of nematodes (nematoda) associated with economically important crops of Kashmir Valley, Jammu and Kashmir. Pakistan Journal of Nematology, 39, 24–40.

    Google Scholar 

  • Huson, D. H., & Scornavacca, C. (2012). Dendroscope 3: An interactive tool for rooted phylogenetic trees and networks. Systematic Biology, 61, 1061–1067.

    Article  Google Scholar 

  • Hunt, D. J. (1993). Aphelenchida, Longidoridae and Trichodoridae: Their systematics and bionomics, CAB International, Wallingford: 352.

  • Hooper D. J. (1966). Longidorus profundorum n. sp. (Nematoda: Dorylaimidae). Nematologica, 11(1964), 489–495.

  • Jahanshahi Afshar, F., Pedram, M., & Mobasseri, M. (2021). Description of Xiphinema persicum n. sp. (Nematoda: Longidoridae), a X. americanum-group species from Iran. European Journal of Plant Pathology, 159, 783–797.

    Article  Google Scholar 

  • Katoh, K., & Standley, D. M. (2013). MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution, 30, 772–780.

    Article  CAS  Google Scholar 

  • Kiontke, K., Gavin, N. P., Raynes, Y., Roehrig, C., Piano, F., & Fitch, D. H. (2004). Caenorhabditis phylogeny predicts convergence of hermaphroditism and extensive intron loss. Proceedings of the National Academy of Sciences of the United States of America, 101, 9003–9008.

  • Krnjaić, D., Lamberti, F., Krnjaić, S., Agostinelli, A., & Radicci, V. (2000). Three new longidorids (Nematoda: Dorylaimida) from Montenegro. Nematologia Mediterranea, 28, 235–248.

    Google Scholar 

  • Lamberti, F., Lemos, R. M., Agostinelli, A., & d’Addabbo, T. (1993). The Xiphinema Americanum-group in the vineyards of the Dao and Douro regions (Portugal) with description of two new species (Nematoda, Dorylaimida). Nematologia Mediterranea, 21, 215–225.

    Google Scholar 

  • Larget, B., & Simon, D. L. (1999). Markov chain Monte Carlo algorithms for theBayesian analysis of phylogenetic trees. Molecular Biology and Evolution, 16, 750–759.

    Article  CAS  Google Scholar 

  • Lazarova, S. S., Elshishka, M., Radoslavov, G., Lozanova, L., Hristov, P., Ladenov, A., Zheng, J., Fanelli, E., De Luca, F., & Peneva, V. K. (2019). Molecular and morphological characterization of Longidorus polyae sp. n. and L. pisi Edward, Misra & Singh, 1964 (Dorylaimida, Longidoridae) from Bulgaria. ZooKeys, 830, 75–98.

    Article  Google Scholar 

  • Loof, P. A., & Chen, Q. W. (1999). A revised polytomous key for the identification of species of the genus Longidorus Micoletzky, 1922 (Nematoda: Dorylaimoidea). Supplement 1. Nematology, 1, 55–59.

    Article  Google Scholar 

  • Loof, P. A. A., & Coomans, A. (1972). The oesophageal gland nuclei of Longidoridae (Dorylaimida). Nematologica, 18, 213–233.

    Article  Google Scholar 

  • Lamberti, F., & Agostinelli, A. (1993). Longidorus raskii sp.n. (Nematoda: Dorylaimida) from Switzerland. Nematologia Mediterranea, 21, 243–246.

  • Lamberti, F., Coiro, M. I., & Agostinelli A. (1991). Longidorus athesinus sp.n. (Nematoda: Dorylairnida) froin northern Italy. Nematologia Mediterranea, 19, 163–1 67.

  • Lamberti, F., Kunz, P., Grunder, J., Molinari, S., De Luca, F., Agostinelli, A., & Radicci, V. (2001). Molecular characterisation of six Longidorus species with the description of Longidorus helveticus sp. n. (Nematoda,Dorylaimida). Nematologia Mediterranea, 29, 181–205.

    Google Scholar 

  • Lamberti, F., Bleve-Zacheo, T., & Arias, M. (1982). The Longidoridae of the Maltese islands with the description of Longidorus magnus sp. n. and Xiphinema melitense sp. n. Nematologia Mediterranea, 10, 183–200.

    Google Scholar 

  • Luc, M., Lima, M. B., Weischer, B., & Flegg, J. J. M. (1964). Xiphinema vuittenezi n. sp. (Nematoda: Dorylaimidae). Nematologica, 10, 151–63.

  • Micoletzky, H. (1922). Die freilebenden Erd-Nematoden. Arch. Naturgesch. Berlin (1921) 87, Abt. A (8/9): 1–650.

  • Nunn, G. B. (1992). Nematode molecular evolution and investigation of evolutionary patterns among nematodes based on DNA sequences. University of Nottingham.

    Google Scholar 

  • Nylander, J. A. A. (2004). MrModeltest v2. Program distributed by the author, Evolutionary Biology Centre, Uppsala University.

  • Pedram, M. (2018). Nematodes of the family Longidoridae. In: Ghaderi, R., Kashi, L. & Karegar, A. (Eds). Plant-parasitic nematodes in Iran. Science Reference in collaboration with the Iranian Society of Nematology, 627–667. (In Persian.)

  • Peneva, V., Lazarova, S. S., De Luca, F., & Brown, D. J. F. (2013). Description of Longidorus cholevae sp. n. (Nematoda: Dorylaimid) from riparian habitat in Rila Mountains, Bulgaria. ZooKeys, 330, 1–26.

    Article  Google Scholar 

  • Roca, F., Lamberti, F., & Agostinelli, A. (1984). Three new species of Longidorus (Nematoda, Dorylaimida) from Italy. Nematologia Mediterranea, 12, 187–200.

    Google Scholar 

  • Robbins, R. T., Brown, D. J. F., Halbrendt, J. M., & Vrain, T. C. (1995). Compendium of Longidorus juvenile stages with observations on L. pisi, L. taniwha, and L. diadecturus (Nematoda: Longidoridae). Systematic Parasitology, 32, 33–52.

    Article  Google Scholar 

  • Ronquist, F., & Huelsenbeck, J. P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.

    Article  CAS  Google Scholar 

  • Rubtsova, T. V., Subbotin, S. A., Brown, D. J. F., & Moens, M. (2001). Description of Longidorus sturhani sp. n. (Nematoda: Longidoridae) and molecular characterisation of several longidorid species from western Europe. Russian Journal of Nematology, 9, 127–136.

    Google Scholar 

  • Siddiqi, M. R., Hooper, D. J., & Khan, E. (1963). A new nematode genus Paralongidorus (Nematoda: Dorylaimoidea) with descriptions of two new species and observations on Paralongidorus citri (Siddiqi 1959) n. comb. Nematologica, 9, 7–14.

    Article  Google Scholar 

  • Širca, S., Urek, G., Lazarova, S., Elshishka, M., & Peneva, V. (2011). Longidorus carniolensis sp. n. (Nematoda,Longidoridae) from vineyard soil in Slovenia. ZooKeys, 141, 1–27.

  • Sturhan, D., & Lišková, M. (2002). Longidorus uroshis Krnjaić et al., 2000 (Nematoda, Dorylaimida) in the Slovak Republic. Helminthologia, 39, 233–236.

  • Subbotin, S., Halford, P. D., Warry, A., & Perry, R. N. (2000). Variations in ribosomal DNA sequences and phylogeny of Globodera parasitising solanaceous plants. Nematology, 2, 591–604.

    Article  CAS  Google Scholar 

  • Subbotin, S., Rogozhin, E., & Chizhov, V. (2013). Molecular characterisation and diagnostics of some Longidorus species (Nematoda: Longidoridae) from Russia and other countries using rRNA genes. European Journal of Plant Pathology, 138, 377–390.

    Article  Google Scholar 

  • Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, 1 and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739.

    Article  CAS  Google Scholar 

  • Wyss, U. (2010). Feeding behavior of plant-parasitic nematodes. In D. L. Lee (Ed.), The biology of nematodes (pp. 462–513). Taylor & Francis Group.

    Google Scholar 

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Acknowledgements

We acknowledge Tarbiat Modares University (Iran) and Science Research Branch Islamic Azad University (Iran) and for their financial support. The study was partly supported by the project ANIDIV4, funded by the Bulgarian Academy of Sciences.

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Authors and Affiliations

  1. Department of Plant Protection, College of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Shahrbanou Bakhshi Amrei & Farshad Rakhshandehroo

  2. Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2, Gagarin Street, 1113, Sofia, Bulgaria

    Vlada Peneva

  3. Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

    Majid Pedram

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  1. Shahrbanou Bakhshi Amrei
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Correspondence to Majid Pedram.

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Bakhshi Amrei, S., Peneva, V., Rakhshandehroo, F. et al. Description of Longidorus armeniacae n. sp. (Nematoda: Longidoridae), associated with Prunus armeniaca L. in Semnan province, Iran. Eur J Plant Pathol 162, 739–750 (2022). https://doi.org/10.1007/s10658-021-02435-0

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