Abstract
Sanqi ginseng Fusarium root rot caused by Fusarium oxysporum, is a serious soil-borne disease that threatens the sustainable development of the sanqi ginseng industry. This study determined the distinct soil bacterial communities from healthy and diseased plant rhizospheres. The ability of beneficial bacteria isolated from the diseased plant rhizosphere with different inoculation concentrations to control Fusarium root rot was investigated. The evenness and Shannon diversity indexes of bacteria in the diseased plant rhizosphere soil were significantly lower than those in the healthy plant rhizosphere soil because the bacterial communities were enriched in the Bacteroidetes and Proteobacteria phyla. The relative abundances of some genera such as Chryseobacterium, Flavobacterium, Stenotrophomonas, and Lysobacter were significantly larger in the diseased plant rhizosphere than in the healthy plant rhizosphere. Two strains, Bacillus sp. KW6 and Lysobacter sp. KW8, isolated from the diseased plant rhizosphere soil exhibited strong antagonistic activity against F. oxysporum, producing chitinase, cellulase, and β-1,3-glucanase. Bacillus sp. KW6 with an inoculation concentration of 108 colony-forming units/g of soil exhibited the largest suppression in the control of sanqi ginseng root rot. The disease incidence of this treatment was only 38.89%, which was 59% lower than that of the control. Sanqi ginseng fresh weight increased with increasing inoculation concentration in treatments applied with antagonistic strains. The amounts of pathogen in stems and roots from treatments inoculated with antagonistic strains were significantly lower than the control.
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Acknowledgements
We really thank the associate professor Jun Zhao and PhD Baoying Wang from Nanjing Normal University for helping us to analyze the sequencing data. This research was supported by Program of the Provincial Education Department of Yunnan Province (2015Y107), the 58th China Postdoctoral Science Foundation (2015M582765XB), Applied Basic Research Foundation of Yunnan Province (2016FB076 and 2016FD019), and Collaborative Innovation Center for Renewable Energy R&D in Southwestern China (05300205020516009). Founders were involved no role in the experimental design, data collection, and the decision to prepare a manuscript.
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Dong, Y., Tang, B., He, M. et al. High concentrations of antagonistic bacterial strains from diseased sanqi ginseng rhizosphere suppressed Fusarium root rot. Eur J Plant Pathol 163, 143–153 (2022). https://doi.org/10.1007/s10658-022-02463-4
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DOI: https://doi.org/10.1007/s10658-022-02463-4