The expression of VISTA on CD4+ T cells associate with poor prognosis and immune status in non-small cell lung cancer patients

Shengyao Ma; Liya Qin; Xinling Wang; Weiyu Wang; Jinfeng Li; Huaijie Wang; Hanyue Li; Xiaoshan Cai; Yang Yang; Meihua Qu

doi:10.17305/bjbms.2021.6531

Authors

Shengyao Ma School of Pharmacy, School of Life Science and Technology, Weifang Medical University, Weifang, China; Translational Medical Center, Weifang Second People’s Hospital, The Second Affiliated Hospital of Weifang Medical University, Weifang, China https://orcid.org/0000-0002-9084-2126
Liya Qin School of Pharmacy, School of Life Science and Technology, Weifang Medical University, Weifang, China; Translational Medical Center, Weifang Second People’s Hospital, The Second Affiliated Hospital of Weifang Medical University, Weifang, China https://orcid.org/0000-0003-0526-1843
Xinling Wang Translational Medical Center, Weifang Second People’s Hospital, The Second Affiliated Hospital of Weifang Medical University, Weifang, China https://orcid.org/0000-0002-9916-5686
Weiyu Wang Translational Medical Center, Weifang Second People’s Hospital, The Second Affiliated Hospital of Weifang Medical University, Weifang, China https://orcid.org/0000-0002-8367-5892
Jinfeng Li Cancer Research Institute of The Fifth Medical Center, The General Hospital of the PLA, Beijing, China https://orcid.org/0000-0002-7430-5574
Huaijie Wang Translational Medical Center, Weifang Second People’s Hospital, The Second Affiliated Hospital of Weifang Medical University, Weifang, China https://orcid.org/0000-0001-8459-0439
Hanyue Li School of Pharmacy, School of Life Science and Technology, Weifang Medical University, Weifang, China; Translational Medical Center, Weifang Second People’s Hospital, The Second Affiliated Hospital of Weifang Medical University, Weifang, China https://orcid.org/0000-0003-0780-7598
Xiaoshan Cai Translational Medical Center, Weifang Second People’s Hospital, The Second Affiliated Hospital of Weifang Medical University, Weifang, China https://orcid.org/0000-0001-5142-8611
Yang Yang School of Public Health, Qingdao University, Qingdao, China https://orcid.org/0000-0003-4751-7253
Meihua Qu Translational Medical Center, Weifang Second People’s Hospital, The Second Affiliated Hospital of Weifang Medical University, Weifang, China https://orcid.org/0000-0003-2464-6773

DOI:

https://doi.org/10.17305/bjbms.2021.6531

Keywords:

VISTA, CD4 T cells, Non-small cell lung cancer, Prognosis, Immune status

Abstract

Besides the two main histologic types of papillary thyroid carcinoma (PTC), the classical PTC (CL-PTC) and the follicular variant PTC (FV-PTC), several other variants are described. The encapsulated FV-PTC variant was recently reclassified as noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) due to its similarities to benign lesions. Specific molecular signatures, however, are still unavailable. It is well known that improper DNA repair of dysfunctional telomeres may cause telomere-related genome instability. The mechanisms involved in the damaged telomere repair processing may lead to detrimental outcomes, altering the three-dimensional (3D) nuclear telomere and genome organization in cancer cells. This pilot study aimed to evaluate whether a specific 3D nuclear telomere architecture might characterize NIFTP, potentially distinguishing it from other PTC histologic variants. Our findings demonstrate that 3D telomere profiles of CL-PTC and FV-PTC were different from NIFTP and that NIFTP more closely resembles follicular thyroid adenoma (FTA). NIFTP has longer telomeres than CL-PTC and FV-PTC samples, and the telomere length of NIFTP overlaps with that of the FTA histotype. In contrast, there was no association between BRAF expression and telomere length in all tested samples. These preliminary findings reinforce the view that NIFTP is closer to non-malignant thyroid nodules and confirm that PTC features short telomeres.