Abstract
Campomanesia phaea (O. Berg) Landrum (Myrtaceae), known as cambuci, is a native species from the Brazilian Atlantic Forest with great potential to be developed as a new fruit crop. Microsatellite markers were developed for cambuci to characterize the genetic diversity and to investigate the genetic structure of a group of accessions originally collected at the presumed center of the species diversity. This work involved the collection of 145 accessions from five regional groups (Juquitiba, Paraibuna, Mogi das Cruzes, Ribeirão Pires, and Salesópolis) in São Paulo state, Brazil. Fourteen loci were identified in an enriched genomic library developed from one of these accessions. Six of the 14 loci were polymorphic, disclosing 26 alleles. Based on the allele frequencies, the calculated genetic parameters of the five groups indicated an average allele number per locus (A) of 3.83, with an expected heterozygosity (He) of 0.57 and observed heterozygosity (Ho) of 0.54. Analysis of the genetic structure indicated that most of the genetic diversity was found within population (HS = 0.57), whereas the genetic diversity among populations was low (GST = 0.19). The genetic diversity parameter of Nei was considered low for the cambuci analyzed populations, with no evidence of inbreeding. Based on Darwin analysis, 18 accessions were chosen from the five regional populations to compose a core collection that included most of the genetic diversity found in this study. Our findings may contribute to defining better conservation strategies and genetic breeding approaches for this native species in Brazil.
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The datasets of the current study are presented in this article or are available upon a reasonable request.
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Acknowledgements
The authors thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the MSc scholarship for the first author and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Grant No. 2014/126063, for financial support for this research. The authors also thank Instituto H & H Fauser, Instituto Auá de Empreendedorismo Socioambiental, Instituto Ecofuturo/Parque das Neblinas, Parque Andreense, Parque Nascentes de Paranapiacaba, Instituto Florestal/ Secretaria de Infraestrutura, and Meio Ambiente do Estado de São Paulo for their technical support during this research.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq): MSc scholarship to the first author; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Grant No. 2014/126063: financial support to this research.
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Rafael Oliveira Moreira: Investigation, Data curation, Writing—Original Draft. Eduardo de Andrade Bressan: Investigation, Writing—Review & Editing. Horst Bremer Neto: Investigation, Data curation. Angelo Pedro Jacomino: Project administration, Funding acquisition. Antonio Figueira: Conceptualization, Writing—Review & Editing. Francisco de Assis Alves Mourão Filho: Conceptualization, Project administration, Writing—Review & Editing.
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Moreira, R.O., de Andrade Bressan, E., Bremer Neto, H. et al. Genetic diversity of cambuci [Campomanesia phaea (O. Berg) Landrum] revealed by microsatellite markers. Genet Resour Crop Evol 69, 1557–1570 (2022). https://doi.org/10.1007/s10722-021-01318-x
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DOI: https://doi.org/10.1007/s10722-021-01318-x